Kif3a (NM_001290806) Mouse Untagged Clone

CAT#: MC228698

Kif3a (untagged) - Mouse kinesin family member 3A (Kif3a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif3a
• Synonyms: Kif3; Kifl; Kns3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228698 representing NM_001290806
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGATCAATAAGTCGGAGAAGCCGGAAAGCTGCGATAATGTGAAGGTGGTGGTTAGGTGCCGGCCTC
  TCAATGAGAGAGAGAAGTCAATGTGCTATAGACAGGCCGTCAGCGTGGATGAAATGAGGGGAACTATCAC
  CGTCCATAAGACCGATTCCTCCAACGAACCTCCAAAGACATTTACTTTCGATACTGTTTTTGGACCAGAA
  AGTAAACAACTTGATGTGTATAACTTAACTGCAAGACCAATTATTGATTCTGTTCTGGAAGGCTACAATG
  GGACCATCTTTGCATATGGACAGACTGGGACAGGCAAGACTTTCACCATGGAAGGCGTTCGAGCAGTACC
  GGGGCTCAGAGGGGTCATCCCCAACTCATTTGCTCACATATTTGGTCACATCGCAAAAGCAGAGGGCGAC
  ACAAGGTTTTTGGTTCGAGTGTCATACTTGGAGATATATAATGAAGAAGTTCGAGACCTTTTGGGTAAAG
  ATCAGACCCAGAGGTTAGAGGTTAAAGAAAGGCCTGATGTGGGAGTATATATCAAAGACTTATCAGCATA
  TGTAGTCAACAACGCTGACGACATGGATAGAATCATGACTCTAGGCCACAAAAACCGTTCTGTCGGTGCG
  ACTAATATGAACGAGCACAGCTCCCGATCCCACGCCATCTTTACAATTACTATAGAGTGCAGTGAGAAAG
  GCGTGGACGGGAACATGCACGTCAGGATGGGCAAGCTCCACCTTGTGGATCTGGCTGGGTCAGAAAGACA
  AGCAAAAACTGGAGCCACAGGACAGCGCCTCAAGGAAGCTACCAAAATCAACCTTTCATTGTCTACCCTG
  GGCAACGTTATTTCTGCCTTGGTGGATGGAAAAAGCACTCACGTGCCGTATCGGAACTCCAAACTGACCC
  GTCTCCTGCAGGACTCCTTAGGAGGGAACTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGATTA
  TAATTATGATGAAACTATCAGCACCTTGCGGTATGCCAACCGCGCCAAGAACATTAAAAACAAAGCGAGA
  ATCAATGAGGACCCAAAGGATGCTCTGCTCCGCCAGTTTCAGAAAGAAATCGAAGAGCTGAAGAAAAAGC
  TTGAGGAAGGGGAAGAAGTGTCAGGCTCTGACATCAGTGGGTCAGAGGAGGATGACGAGGAGGGTGAACT
  TGGAGAAGATGGAGAAAAAAAGAAAAAGAGAAGGGACCAAGCAGATAAGATGGTGGAAATGCAAGCGAAA
  ATTGACGAGGAGAGAAAAGCACTGGAAACCAAACTTGACATGGAAGAAGAAGAAAGAAACAAGGCTAGAG
  CTGAACTAGAGAGACGGGAGAAGGACCTTCTGAAAGCCCAACAAGAGCATCAGTCTTTGCTGGAAAAGCT
  GTCTGCTTTGGAAAAGAAGGTCATTGTCGGCGGCGTGGACCTGCTGGCCAAGGCTGAGGAGCAAGAGAAG
  CTTCTGGAGGAGTCCAACATGGAGCTGGAGGAGAGGAGGAGACGAGCTGAGCAGCTTCGGAAAGAGCTGG
  AGGAAAAGGAGCAAGAACGCTTGGACATCGAGGAAAAATATACCAGTCTGCAAGAGGAGGCACAAGGAAA
  GACCAAGAAATTAAAGAAAGTCTGGACCATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTCCAGCAG
  GAGCATCAGAGGGAGATCGAAGGCCTCCTGGAGAACATCCGGCAGCTCAGCCGGGAGCTCCGTCTTCAGA
  TGCTCATCATTGATAACTTCATACCTCAGGATTACCAGGAAATGATTGAAAACTACGTCCACTGGAATGA
  AGACATAGGAGAATGGCAGCTAAAATGTGTGGCCTACACAGGAAATAACATGAGGAAACAAACTCCAGTC
  CCTGACAAGAAGGAGAGAGACCCATTTGAAGTCGACCTTTCCCACGTGTACCTCGCCTATACAGAGGAGA
  GTCTGCGTCAGTCTCTGATGAAGTTAGAAAGGCCACGGACCTCCAAGGGGAAAGCAAGGCCAAAGATGGG
  GCGAAGAAAGCGTTCTGCAAAGCCTGAGACCGTAATTGATTCTTTACTTCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290806
• Insert Size: 2085 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290806.1, NP_001277735.1
• RefSeq Size: 5429 bp
• RefSeq ORF: 2085 bp
• Locus ID: 16568
• UniProt ID: P28741
• Cytogenetics: 11 31.97 cM
• Gene Summary: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (PubMed:21670265). Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole (PubMed:23386061).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.