Ppp2r1b (NM_001286553) Mouse Untagged Clone

CAT#: MC228697

Ppp2r1b (untagged) - Mouse protein phosphatase 2, regulatory subunit A, beta (Ppp2r1b), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp2r1b
• Synonyms: 2410091N08Rik; AI790395
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228697 representing NM_001286553
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCAGCAGGGCCAGGGTCCGGTCCGGGGGCAGCGGGTGGAGATGGAGACGATTCGCTCTACC
  CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAGGACGTGCAGCTCCGTCTCAACAGTATTAAGAAGTT
  ATCAACTATAGCTCTAGCACTCGGGGTAGAAAGGACTCGAACGGAACTGCTGCCTTTCCTTACAGATACT
  ATTTATGATGAAGATGAGGTGCTGTTAGCTCTCGCCGAGCAGCTGGGAAATTTCACTGGTTTGGTGGGCG
  GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAGTTTGGCTACGGTGGAGGAGACTGTCGTTCG
  AGACAAGGCTGTGGAGTCGCTGAGGCAGATCTCGCAGGAGCACACTCCTGTTGCCCTTGAAGCTCATTTT
  GTGCCTCTGGTGAAGCGCCTGGCAAGCGGGGACTGGTTCACGTCGCGCACGTCTGCATGTGGCTTGTTCA
  GCGTTTGCTATCCCAGAGCTTCAAATGCTGTCAAGGCAGAGATTCGACAGCACTTCCGTTCTCTCTGCTC
  GGATGATACACCAATGGTGCGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA
  GACAGTGTGAAAACTGAAATTGTCCCATTGTTCACGAATTTGGCTTCAGATGAACAGGACTCTGTGCGGC
  TGCTAGCTGTGGAAGCCTGCGTCAGCATTGCCCAGTTACTGTCTCAGGAGGACCTGGAAGCCTTGGTAAT
  GCCTACACTTCGACAAGCAGCAGAAGATAAGTCCTGGCGGGTTCGATATATGGTGGCTGACAAGTTTTCA
  GAGCTCCAGAAAGCGGTGGGCCCTAAGATTGCACTCAGCGACCTCATCCCCGCCTTCCAGAGCCTCCTCC
  GAGACTGCGAAGCTGAAGTCCGAGCAGCCGCTGCCCACAAAGTAAGAGAGCTTTGTGAGAACCTGCCCGC
  TGAAGGTAGAGAGACTGTAATTATGAATCAAATCCTGCCCTATATAAAGGAATTAGTGTCTGATACCAAC
  CAACATGTCAAGTCAGCGCTAGCTTCTGTAATTATGGGATTATCTACAGTTTTGGGCAAAGAGAATACCA
  TTGAACATCTTCTGCCTCTATTTTTAGCTCAGTTAAAGGACGAGTGTCCAGAAGTTCGTTTAAATATCAT
  CTCCAATTTGGATTGTGTGAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCGGCCATA
  GTGGAACTGGCTGAAGATGCCAAGTGGAGGGTCCGCCTGGCTATCATTGAGTACATGCCGCTGCTGGCTG
  GCCAGCTGGGTGTGGAGTTCTTTGATGAAAAACTCAATTCTTTGTGTATGGCCTGGCTTGTGGATCATGT
  CTATGCCATTCGGGAGGCAGCCACCAACAACCTCATGAAGTTAGTCCAGAAGTTCGGTACAGAGTGGGCC
  CAGAATACCATTGTCCCTAAAGTGTTGGTAATGGCAAATGACCCTAACTACTTACACAGAATGACCACGC
  TCTTCTGCATTAATGCACTGTCTGAAGCCTGTGGTAAGGAAATAACCACTAAGCAAATGTTGCCCATCGT
  GTTGAAAATGGCAGGAGACCAGGTAGCAAATGTCCGCTTCAATGTGGCCAAATCACTGCAGAAAATTGGA
  CCGATTCTAGACACCAACGCCTTGCAGGGGGAAGTGAAGCCGGTGCTGCAGAAATTGGGTCAGGACGAAG
  ACATGGATGTCAAATATTTTGCACAGGAAGCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGATTT
  TCCTGTGAAGGACAGTGAAGAGCCCAGCATCCCTGGGGTTGACAAGAATCTCTTCCTGAGACCCCGAGGG
  CCTGGAGAGGACACTGGGAAGGCACAGAGAGACGGAGCGGCTCCAGTGGCTTCCGAGGAGGCAGTAGAGG
  CAGCCCAGCTTAGGGGCACAAACACAGCAAAGGGACCAGCATGTCAGTTGCATATAAATACTAGAGACAC
  ACTTGCCCAACTGGAAATTGCAGAAGCAATGCATTTCCCCCAAAGCAGAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286553
• Insert Size: 2085 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286553.1, NP_001273482.1
• RefSeq Size: 3627 bp
• RefSeq ORF: 2085 bp
• Locus ID: 73699
• Cytogenetics: 9 A5.3
• Gene Summary: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) encodes the longest isoform (c).