Bcl11b (NM_001286343) Mouse Untagged Clone

CAT#: MC228686

Bcl11b (untagged) - Mouse B cell leukemia/lymphoma 11B (Bcl11b), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bcl11b
• Synonyms: 9130430L19Rik; AI604821; B630002E05Rik; BCL-11B; Ctip2; Rit1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228686 representing NM_001286343
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGCCGCAAACAGGGCAACCCGCAGCACTTGTCCCAGAGGGAACTCATCACGCGTAAAGATGAGC
  CTTCCAGCTACATTTGCACAACATGCAAGCAGCCCTTCAACAGCGCCTGGTTCCTGCTGCAGCACGCACA
  GAACACACATGGCTTCCGAATCTACCTGGAGCCTGGGCCGGCCAGCACCTCGCTCACGCCCAGGCTCACC
  ATCCCGCCACCGCTCGGGCCGGAGACCGTGGCGCAGTCCCCACTCATGAATTTCCTGGGGGACAGCAATC
  CTTTCAACCTGCTGCGCATGACGGGCCCCATCCTGCGGGACCACCCTGGCTTCGGTGAGGGCCGCTTGCC
  AGGTACGCCACCGCTCTTCAGCCCACCGCCACGCCATCACTTGGACCCACACCGCCTCAGTGCAGAGGAG
  ATGGGGCTCGTGGCCCAGCACCCCAGTGCCTTCGACCGAGTCATGCGCCTGAACCCCATGGCCATAGACT
  CTCCTGCCATGGACTTCTCCCGGCGGCTGCGAGAACTGGCCGGCAACAGCTCCACGCCGCCGCCCGTGTC
  CCCAGGCCGTGGCAACCCTATGCACCGGCTGCTGAACCCTTTCCAGCCCAGTCCCAAGTCCCCGTTCCTC
  AGCACGCCACCGCTGCCACCCATGCCTGCGGGCACACCGCCACCGCAGCCGCCTGCCAAGAGCAAGTCCT
  GTGAGTTCTGCGGCAAGACCTTCAAGTTCCAGAGCAATCTCATCGTGCACCGGCGCAGCCACACGGGCGA
  GAAGCCCTACAAGTGCCAGCTGTGCGACCATGCGTGCTCGCAGGCGAGCAAGCTCAAGCGCCACATGAAG
  ACGCACATGCACAAGGCGGGCTCTCTGGCTGGCCGCTCAGACGACGGGCTCTCAGCTGCCAGCTCCCCTG
  AGCCGGGCACCAGCGAGCTGCCAGGTGACCTGAAAGCGGCCGATGGCGACTTCCGCCACCATGAGAGCGA
  CCCATCTCTGGGCCCCGAGCCTGAGGACGACGAGGACGAGGAGGAGGAAGAAGAGGAGCTGCTGCTGGAG
  AACGAGAGCCGGCCTGAGTCGAGCTTCAGCATGGACTCGGAGCTGGGCCGTGGCCGCGAGAACGGAGGTG
  GCGTGCCACCGGGGGTGGCGGGCGCAGGGGCTGCAGCTGCGGCTCTGGCGGATGAGAAGGCTCTGGCCCT
  GGGCAAGGTGATGGAGGACGCAGGGCTGGGCGCACTGCCGCAGTATGGGGAGAAGCGGGGCGCCTTCCTG
  AAGCGTGCAGGCGACACGGGTGATGCCGGAGCTGTTGGCTGTGGGGACGCGGGTGCACCGGGTGCAGTGA
  ACGGGCGCGGCGGGGCCTTCGCGCCAGGCGCAGAGCCCTTTCCAGCTCTCTTCCCACGCAAGCCAGCACC
  GCTGCCCAGCCCTGGGCTCGGTGGTCCCGCGCTGCACGCGGCCAAGCGCATCAAGGTGGAGAAAGACCTG
  GAGCTGCCACCTGCCGCCCTCATCCCATCTGAGAACGTGTACTCGCAGTGGCTCGTGGGCTACGCAGCAT
  CGCGCCACTTCATGAAGGACCCATTCCTGGGCTTCACGGATGCGCGCCAGTCGCCTTTCGCCACATCGTC
  GGAACATTCCTCTGAGAACGGCAGCCTGCGCTTCTCAACGCCACCCGGGGACCTGCTGGACGGCGGGCTG
  TCCGGGCGCAGTGGCACGGCGAGCGGGGGCAGCACACCTCACCTGGGTGGTCCGGGTCCTGGGAGGCCGA
  GCTCCAAGGAGGGCCGCCGCAGCGACACATGTGAGTACTGCGGCAAGGTCTTCAAGAACTGTAGCAACCT
  GACGGTGCACCGGAGGAGCCACACCGGCGAGCGGCCTTACAAGTGCGAGCTGTGCAACTACGCGTGCGCG
  CAGAGCAGCAAGCTCACGCGCCACATGAAGACGCACGGGCAGATCGGCAAGGAGGTGTACCGCTGCGACA
  TCTGCCAGATGCCCTTCAGCGTCTACAGCACCCTGGAGAAACACATGAAAAAGTGGCACGGTGAACACTT
  GCTGACTAATGATGTCAAAATCGAGCAGGCTGAGAGGAGCTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001286343
• Insert Size: 2073 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286343.1, NP_001273272.1
• RefSeq Size: 7341 bp
• RefSeq ORF: 2073 bp
• Locus ID: 58208
• UniProt ID: Q99PV8
• Cytogenetics: 12 59.1 cM
• Gene Summary: Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals (PubMed:12717433). Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (By similarity). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two internal coding exons but maintains the reading frame, compared to variant 1. The resulting isoform (c) lacks an internal segment, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.