Amph (NM_001289546) Mouse Untagged Clone

CAT#: MC228684

Amph (untagged) - Mouse amphiphysin (Amph), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amph
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228684 representing NM_001289546
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACATCAAGACGGGCATCTTCGCCAAAAACGTGCAAAAGCGCCTCAACCGCGCGCAGGAAAAGG
  TCCTCCAGAAGCTGGGGAAGGCTGACGAGACAAAAGATGAACAGTTTGAAGAATATGTCCAGAACTTCAA
  ACGGCAGGAGGCAGAGGGTACCAGACTTCAGCGAGAACTCCGGGGATATTTAGCAGCAATCAAAGGTATG
  CAGGAGGCCTCAATGAAGCTCACTGAATCTCTACATGAAGTCTACGAGCCAGACTGGTATGGACGGGAAG
  ATGTAAAGATGGTTGGTGAGAAGTGTGATGTGCTGTGGGAAGACTTCCATCAAAAGCTAGTGGATGGCTC
  CTTGCTGACACTGGACACCTACCTGGGCCAGTTTCCTGACATAAAGAATCGCATCGCTAAGCGCAGCAGG
  AAGCTTGTGGATTATGATAGTGCTCGCCATCACCTGGAGGCTCTGCAGAGTTCCAAACGGAAAGATGAGA
  GTCGGATCTCTAAGGCAGAAGAGGAGTTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTCAATGTTGACTT
  ACAAGAAGAGTTGCCGTCATTGTGGTCAAGTCGAGTTGGCTTTTACGTCAATACTTTCAAAAATGTCTCC
  AGCCTTGAGGCCAAGTTTCATAAGGAAATCGCAGTGCTTTGCCACAAGCTCTATGAAGTGATGACGAAAC
  TCGGTGATCAGCATGCTGACAAGGCCTTCAGCATTCAAGGAGCTCCCAGTGACTCGGGTCCTCTTCGCAT
  TGCAAAGACACCATCACCTCCAGAGGAGCCTTCTCCCCTGCCCAGCCCCACGGCTAGTCCCAACCACACA
  TTAGCACCTGCATCTCCTGCCCCAGTGCGACCCAGATCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC
  CACCTCTGCCTAAAGTCACCCCAACAAAGGAACTGAAACAGGAGAACATCATCAATTTCTTTGAGGACAA
  CTTTGTACCAGAAATCAATGTGACAACACCTTCCCAGAACGAAGTCTTGGAGGTGAAGAAGGAAGAGACT
  TTGCTGGATTTGGACTTTGACCCTTTCAAGCCCGATGTGGCTCCGGCAGGTTCTGCTGCAGCGACCCACT
  CTCCCATGTCTCAGACATTGCCCTGGGACTTATGGACGACAAGCACTGATTTGGTACAACCAGCTTCTGG
  GGGTTCATTTAATGATTTCACCCAGGCCCAGGACACTTCATTATTCACCATGCAGACAGATCAGAATATG
  GTAGGTGGCTTGGCTGAAACTGAGCAGGCTCTACCTACAGAGCCCCAAGCCGAAGAGCCTCCTGCCACTG
  CTGCTGCACCTACTGCTGGGCTGGACCTTGGGCTAGAGATGGAGGAGCCAAAGGAGGAGGCTGTGATCCC
  TCCCGCAACTGATACTGGCGAGACTGTGGAAACTGCAGTGCCAACTGAGGGTGCCCCGGTGGAGGAAGCA
  GAGGCAGAGAAGGCAGCCCTCCCTGCTGGAGAAGGAGGAAGTCCAGAGGGAGCCAAAATTGACGGGGAAA
  GCACGGAGCTTGCAATCAGTGAGAGCCCTCAACCAGTGGAGCCCGAAGCGGGTGCTCCTCAGGTCATCCC
  TTCTGTTGTCATTGAGCCAGCCTCCAACCATGAAGGAGAAGGAGAACACCAAGAAACTGCTACGGGTACT
  GAACCGAGGGAGGCCGCTGAGGATGTGGCTGCCCAGGGCTCAGCGGGTGAGAAGCAGGAAGTGGCTACAG
  AGCCCACGCCTCTGGATTCTCAGGCAACTCTGCCTGCATCTGCAGGTGCTGTGGATGCCTCGCTGTCTGC
  AGGGGATGCCACTCAGGAACTGCCTCCTGGCTTTCTCTACAAGGTGGAAACCCTGCATGATTTCGAGGCA
  GCGAACTCTGATGAACTTAACCTACAAAGGGGTGACGTGGTACTCGTGGTGCCTTCAGACTCAGAAGCCG
  ACCAGGATGCAGGCTGGTTGGTGGGGGTGAAGGAGTCAGACTGGCTTCAGTACAGAGACCTGGCCACGTA
  CAAAGGCCTCTTTCCAGAGAACTTCACGCGGCGCCTGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289546
• Insert Size: 2073 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289546.1, NP_001276475.1
• RefSeq Size: 3247 bp
• RefSeq ORF: 2073 bp
• Locus ID: 218038
• UniProt ID: Q7TQF7
• Cytogenetics: 13 6.89 cM
• Gene Summary: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, which results in a longer protein (isoform 2), compared to isoform 1.