Dyrk1b (NM_001271370) Mouse Untagged Clone

CAT#: MC228680

Dyrk1b (untagged) - Mouse dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1b (Dyrk1b), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dyrk1b
• Synonyms: Mirk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228680 representing NM_001271370
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCCGCTCGCCCACCGCACTGGGGTCCCCATCGCGCTCCAGCCCCCCGTGGGCCCAGCGCCATCC
  CTGACCCGGGTCTCAGCGGCGGTGGCAGCCGAGGTGCAGGATGCGAGAAGGCGCCCCCCGGCCGGGCTCC
  CGCTCCAGGCCTCACGCCCCTGCGGCCCTCTGAGCCCACCATGGCCGTCCCACCAGGCCATGGTCCTTTC
  TCTGGCTTTCCGGGGCCCCAGGAACACACACAGGTACTACCTGATGTGCGGCTCCTGCCCCGGAGACTGC
  CCCTGGCCTTCCGGGATGCGGCCTCAGCCCCGCTGCGCAAGCTCTCGGTGGACCTCATCAAGACCTACAA
  GCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGGGCCCAACAGGCGCCACCCCAGGACTCGAGC
  ACCAAAAAGGAGAAGAAGGTCCTGAACCACGGTTATGATGACGACAACCACGACTACATTGTGCGCAGTG
  GCGAGCGCTGGCTAGAGCGCTATGAGATTGACTCTCTTATTGGCAAAGGCTCCTTTGGCCAGGTGGTGAA
  AGCCTATGATCACCAGACTCAGGAGCTGGTGGCCATCAAGATCATCAAGAACAAAAAGGCCTTCCTGAAC
  CAGGCACAGATTGAGCTACGGCTGTTGGAGCTGATGAACCAGCATGATACAGAGATGAAGTACTACATAG
  TACACCTTAAGCGGCACTTCATGTTCCGGAATCACCTGTGCCTGGTGTTTGAGCTGCTGTCCTACAACCT
  GTACGACCTCCTCCGCAACACACACTTTCGGGGTGTCTCACTGAACCTGACGAGGAAGCTGGCACAGCAG
  CTCTGCACAGCTCTGCTCTTTCTGGCCACCCCCGAGCTCAGCATCATCCACTGCGACCTCAAGCCTGAGA
  ACATCCTGCTCTGCAACCCCAAGCGCAGTGCCATCAAGATCGTGGACTTCGGCAGTTCCTGCCAGCTTGG
  CCAGCGGATCTACCAGTATATCCAGAGCCGCTTCTACCGCTCACCCGAGGTGCTCCTGGGTACACCCTAT
  GACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTCGTGGAGATGCACACCGGAGAGCCCCTCTTCA
  GTGGCTCTAATGAGGTGGACCAGATGAGCCGTATTGTGGAGGTGTTGGGCATCCCTCCCGCACCCATGCT
  GGAACAGGCACCCAAGGCTCGAAAGTACTTTGAGCGGCTGCCTGGGGGTGGCTGGACCCTACGAAGGACA
  AAGGAACTCAGGAAGGATTACCAGGGCCCTGGGACACGGCGGCTGCAGGAGGTGCTGGGCGTGCAGACGG
  GCGGGCCCGGGGGCCGGCGGGCGGGGGAGCCCGGCCACAGCCCCGCCGACTACCTCCGCTTCCAGGACCT
  GGTGCTGCGCATGCTGGAATATGAGCCCGCCGCCCGCATCAGCCCTCTGGGCGCTCTGCAGCATGGCTTC
  TTCCGCCGCACGGCCGACGAGGCCACCAACACGGGCCCGGCAGGCAGCAGTGCCTCCACCTCGCCGGCGC
  CCCTTGACACCTGCCCCTCCTCTAGCACCGCCAGCTCCATCTCCAGCTCTGGAGGTTCCAGTGGCTCCTC
  CAACGACAACAGAGCCTACCGATACAGCAACCGATATTGTGGGGGCCCAGGGCCCCCCATCACTGACTGT
  GAGATGAACAGCCCCCAGGTCCTACCCTCCCAGCCTCTGCGCCCCTGGGCAGGGGGTGATGTGCCCCACA
  AGACACATCAAGCCCCTATCTCTGCCTCAACATTGCCGGGGACTGGGGCTCAGTTACCCCCATTGCCCCG
  TTGCCTTGGACGACCCCCATCACCAACATCACCACCACCCCCAGAGTTGATGGATGTGAGCCTGGTGGGC
  AGCCCTCCAGACTGCTCTCCACCTCCTCCAGCACCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGA
  CTCGGATGACAGGAGGTCGACCACCTCTCCCACCCCCTGATGACCCTGCCACTCTGGGGCCTCGCCTGGG
  TCTCCATGGTGTACCCCAGAGCACAGCAGCCAGCTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271370
• Insert Size: 2070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271370.1, NP_001258299.1
• RefSeq Size: 2477 bp
• RefSeq ORF: 2070 bp
• Locus ID: 13549
• UniProt ID: Q9Z188
• Cytogenetics: 7 A3
• Gene Summary: Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activity. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4), uses an alternate 5' structure, compared to variant 1. This difference causes translation initiation at an upstream AUG and results in an isoform (p75), also known as isoform c, that has a longer N-terminus, compared to isoform p69. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.