Adgrg1 (NM_001198894) Mouse Untagged Clone

CAT#: MC228676

Gpr56 (untagged) - Mouse G protein-coupled receptor 56 (Gpr56), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrg1
• Synonyms: Cyt28; Gpr56; TM7LN4; TM7XN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228676 representing NM_001198894
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCCAGGTGCTGCGGCAGATGGTCTACTTCCTACTGAGTCTGTTTTCTCTGGTGCAAGGTGCAC
  ACAGTGGCAGCCCCCGAGAAGACTTCCGCTTCTGTGGCCAGCGGAACCAGACCCAACAGAGCACCCTCCA
  CTATGATCAATCTTCAGAGCCTCACATCTTTGTGTGGAACACAGAGGAGACCCTCACAATTCGTGCCCCC
  TTCCTGGCAGCCCCAGATATTCCCCGCTTCTTCCCAGAGCCTAGAGGGCTCTATCACTTCTGCCTCTACT
  GGAGTCGCCACACTGGGAGACTCCACTTGCGCTATGGCAAGCATGACTACCTGCTTAGTAGCCAAGCCTC
  CAGACTCCTCTGCTTCCAGAAACAGGAGCAGAGCCTGAAGCAGGGAGCCCCGCTGATCGCCACCTCTGTC
  AGCTCCTGGCAGATTCCCCAGAACACCAGCCTGCCTGGGGCTCCGAGCTTCATCTTCTCCTTCCACAATG
  CCCCACACAAGGTCTCCCACAATGCATCTGTGGACATGTGTGATCTCAAGAAGGAATTGCAGCAGCTTAG
  CAGGTACCTGCAGCACCCTCAAAAGGCTGCCAAGCGGCCCACCGCAGCGTTCATCAGCCAGCAGTTACAG
  AGCCTGGAGTCAAAGCTGACCTCTGTGAGCTTCCTGGGAGACACATTATCCTTTGAGGAGGACCGGGTCA
  ATGCTACAGTGTGGAAGCTGCCACCCACAGCCGGTCTAGAGGATCTGCATATCCACTCCCAGAAGGAGGA
  GGAGCAGAGTGAGGTCCAGGCATACTCGCTGTTGCTTCCCCGGGCCGTATTCCAGCAGACCAGAGGCCGT
  CGCCGGGATGACGCCAAGAGGCTCCTGGTAGTAGACTTCAGCAGCCAAGCTTTGTTCCAGGACAAGAATT
  CTAGCCAAGTCCTGGGTGAGAAGGTCTTGGGTATTGTCGTGCAGAACACCAAAGTCACCAACCTCTCAGA
  TCCGGTGGTACTCACCTTCCAGCACCAGCCTCAGCCAAAAAATGTGACTCTGCAGTGCGTGTTCTGGGTT
  GAAGACCCGGCATCAAGCAGCACAGGGAGCTGGAGCAGTGCAGGCTGCGAGACAGTGAGCAGAGACACAC
  AGACATCCTGCCTGTGCAACCACCTGACCTACTTTGCAGTGCTGATGGTGTCATCCACAGAGGTAGAAGC
  CACTCACAAACACTACCTCACGCTCCTGTCCTACGTGGGCTGTGTCATCTCTGCTCTGGCTTGTGTCTTC
  ACTATTGCTGCCTACCTCTGCTCCAGGAGGAAGTCACGTGACTACACCATCAAAGTCCACATGAACCTGC
  TGTCCGCTGTCTTCCTGCTGGACGTGAGCTTCCTGCTCAGCGAGCCTGTGGCACTGACGGGCTCCGAAGC
  AGCCTGTCGCACCAGTGCCATGTTCCTGCACTTCTCCCTGCTTGCCTGCCTCTCCTGGATGGGCCTCGAG
  GGCTACAATCTCTACCGACTGGTGGTGGAGGTCTTCGGTACCTATGTGCCCGGCTATCTGCTCAAGCTGA
  GCATCGTGGGCTGGGGTTTTCCTGTCTTCCTGGTCACTCTGGTGGCGTTGGTGGATGTGAATAACTACGG
  CCCCATTATCCTAGCTGTGCGCCGGACTCCGGAACGTGTCACCTACCCCTCTATGTGCTGGATCCGGGAC
  TCCCTGGTGAGCTATGTCACCAACCTGGGCCTCTTCAGTCTGGTGTTCCTGTTCAACCTGGCTATGCTGG
  CCACCATGGTGGTGCAGATCCTGCGGCTTCGCCCGCACAGCCAGAACTGGCCCCACGTGCTGACCCTGCT
  GGGCCTCAGCCTGGTCCTTGGCCTCCCCTGGGCCTTGGTCTTCTTTTCCTTTGCTTCCGGCACCTTCCAG
  CTTGTCATCCTCTACCTCTTCAGCATCATAACTTCCTTCCAAGGCTTCCTCATCTTCCTGTGGTACTGGT
  CCATGCGGTTCCAGGCCCAAGGCGGCCCCTCCCCTCTGAAGAACAACTCAGACAGCGCCAAACTCCCCAT
  CAGCTCCGGCAGCACCTCCTCCAGCCGCATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198894
• Insert Size: 2064 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198894.1, NP_001185823.1
• RefSeq Size: 3605 bp
• RefSeq ORF: 2064 bp
• Locus ID: 14766
• UniProt ID: Q8K209
• Cytogenetics: 8 47.12 cM
• Gene Summary: Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination (PubMed:21768377). Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12 (By similarity). Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (PubMed:23478665). Plays a critical role in tumourigenesis (By similarity). Plays essential role in testis development (PubMed:20981830).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.