Cpeb3 (NM_001290829) Mouse Untagged Clone

CAT#: MC228671

Cpeb3 (untagged) - Mouse cytoplasmic polyadenylation element binding protein 3 (Cpeb3), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpeb3
• Synonyms: 4831444O18Rik; CPE-BP3; mKIAA0940
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228671 representing NM_001290829
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGATGATTTACTGATGGACAAAAGCAAAACCCAGCCCCAGTCTCAGCAGCAGCAGCGGCAGCAGC
  AGCAGCAGCAGCAACAGCTCCAGCCCGAGCCCGGCGCAGCTGAAGCCCCGTCCACGCCCCTCTCCTCAGA
  GATCCCCAAGCCCGAAGACAGTAGCGCAGTGCCGGCCCTCAGCCCCGCCTCGGCTCCGCCAGCCCCCAAC
  GGCCCCGACAAGATGCAGATGGAGTCGCCGCTCCTGCCGGGCTTGAGTTTCCATCAGCCCCCTCAGCAGC
  CGCCGCCGCCGCAGGAGCCCACGGCGCCCGGAGCGTCGCTGTCGCCGTCCTTCGGCAGCACCTGGTCCAC
  AGGCACTACAAACGCGGTGGAGGACAGCTTCTTCCAGGGGATCACCCCAGTCAACGGGACCATGCTCTTC
  CAAAACTTCCCGCACCACGTCAACCCGGTCTTCGGAGGCACCTTCTCGCCGCAGATCGGCCTGGCGCAGA
  CCCAGCACCATCAGCAGCCTCCACCGCCCGCGCCGCAGCCGCCGCAGCCCGCGCAGCCCCCGCAGGCGCA
  GCCCTCGCAGCAACGCCGCTCGCCCGCCAGCCCCAGCCAGGCGCCCTATGCGCAGAGGAGCGCCGCTGCC
  TACGGCCACCAGCCCATCATGACCAGCAAGCCGTCCTCATCCTCGGCGGTCGCGGCTGCTGCGGCCGCGG
  CCGCTGCCTCTTCGGCCTCGTCCAGCTGGAACACGCACCAGAGCGTGAACGCCGCCTGGAGTGCTCCGTC
  CAACCCGTGGGGTGGCCTGCAGGCGGGCCGAGACCCTCGCCGCGCGGTCGGCGTGGGCGTGGGTGTAGGT
  GTGGGGGTACCCTCCCCGCTTAACCCCATCTCGCCGCTCAAAAAGCCCTTCTCCAGCAATGTGATCGCTC
  CACCCAAGTTCCCTCGTGCAGCCCCGCTCACCTCCAAGTCCTGGATGGAGGATAACGCTTTCCGGACCGA
  TAATGGTAACAATCTGTTGCCTTTTCAGGACCGGAGTAGGCCCTATGACACTTTTAACTTGCACTCCTTG
  GAGAACTCCTTAATGGATATGATAAGGACTGACCATGAGCCTCTGAAAGGACGCATGGGAATAAATTTCC
  ACCATCCAGGAACAGATAACATTATGGCACTTAACAGTCGGTCTTCTCTCTTTCCCTTTGAAGACGCCTT
  CTTGGATGACAGCCATGGTGATCAGGCCTTATCGTCTGGCTTAAGTTCTCCTACTCGATGTCAAAATGGG
  GAACGAGTGGAACGCTACTCTAGAAAGGTGTTTGTTGGAGGCCTTCCTCCTGACATCGATGAAGATGAGA
  TCACTGCCAGCTTTCGCAGGTTTGGACCCCTTGTGGTGGACTGGCCTCATAAAGCCGAGAGCAAATCGTA
  CTTCCCTCCTAAAGGCTATGCTTTCCTGCTGTTCCAGGAGGAGAGCTCAGTGCAAGCTCTGATAGATGCC
  TGCCTGGAGGAGGATGGGAAACTGTACCTGTGTGTGTCCAGCCCAACCATCAAGGATAAACCGGTGCAAA
  TCCGACCGTGGAACCTAAGTGACAGTGACTTTGTAATGGATGGCTCTCAGCCTTTGGACCCCAGAAAAAC
  TATCTTTGTTGGGGGAGTTCCACGACCCCTTCGAGCTGTTGAACTGGCAATGATAATGGACCGTTTGTAC
  GGTGGTGTTTGCTATGCTGGCATTGACACAGACCCAGAGCTGAAGTACCCGAAAGGTGCTGGGCGTGTTG
  CGTTCTCCAATCAGCAGAGCTACATTGCAGCCATCAGTGCGCGCTTTGTGCAACTTCAACACAACGACAT
  TGACAAACGGGTGGAAGTGAAGCCGTACGTGCTGGATGATCAGATGTGTGACGAGTGTCAGGGCACACGC
  TGCGGGGGGAAGTTTGCCCCATTCTTCTGTGCCAACGTCACCTGTCTGCAGTACTACTGTGAATACTGCT
  GGGCCAGCATCCACTCCCGAGCCGGCCGTGAGTTCCACAAACCGCTGGTGAAGGAGGGAGGCGACCGCCC
  CCGGCACGTCCCGTTCCGCTGGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290829
• Insert Size: 2058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290829.1, NP_001277758.1
• RefSeq Size: 5758 bp
• RefSeq ORF: 2058 bp
• Locus ID: 208922
• UniProt ID: Q7TN99
• Cytogenetics: 19 C2
• Gene Summary: Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (PubMed:17024188, PubMed:26074072). In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3' UTR, but binds to a U-rich loop within a stem-loop structure (PubMed:17024188). Required for the consolidation and maintenance of hippocampal-based long term memory (PubMed:26074003). In the basal state, binds to the mRNA 3' UTR of the glutamate receptors GRIA1 and GRIA2 and negatively regulates their translation (PubMed:17024188, PubMed:22153079). Also represses the translation of DLG4, GRIN1 GRIN2A and GRIN2B (PubMed:24155305). When activated, acts as a translational activator of GRIA1 and GRIA2 (PubMed:22153079, PubMed:26074003). In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (PubMed:26074071). Binds to the 3' UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (PubMed:26915043). Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (PubMed:26074072). Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (By similarity). In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (By similarity). In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, uses an alternate in-frame splice site, and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.