Dtna (NM_001285810) Mouse Untagged Clone

CAT#: MC228669

Dtna (untagged) - Mouse dystrobrevin alpha (Dtna), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dtna
• Synonyms: 2210407P21Rik; a-DB-1; adbn; Dtn; DTN-A; Gm19389
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228669 representing NM_001285810
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGAAGATAGTGGAAAAAGAGGAAACACCATGGCAGAAAGAAGACAGCTGTTTGCAGAGATGAGGG
  CTCAAGATCTGGATCGCATCCGACTCTCCACCTACAGAACAGCATGCAAGCTGAGATTTGTGCAGAAGAA
  ATGCAATTTGCACCTGGTGGACATTTGGAACGTCATTGAAGCATTGCGCGAAAACGCTTTGAATAACCTG
  GACCCAAACATAGAACTCAACGTGGCCCGCCTGGAGGCGGTGCTCTCCACTATTTTTTACCAGCTCAACA
  AGAGGATGCCAACCACTCACCAAATCCACGTGGAGCAGTCCATCAGTCTCCTGCTGAACTTCCTGCTCGC
  AGCCTTTGACCCGGAAGGCCATGGAAAAATCTCAGTATTTGCTGTCAAAATGGCTTTGGCTACATTGTGT
  GGAGGAAAGATCATGGACAAGTTAAGATATATTTTCTCAATGATCTCTGACTCCAGTGGAGTGATGGTAT
  ATGGAAGATATGACCAATTCCTTCGGGAAGTTCTCAAACTACCCACAGCAGTTTTTGAAGGTCCTTCATT
  TGGTTACACAGAACAGTCAGCCAGATCCTGTTTCTCCCAACAGAAAAAGGTCACCTTAAATGGCTTCTTG
  GACACGCTCATGTCAGACCCTCCTCCACAGTGCCTGGTATGGCTGCCTCTTCTGCACCGACTGGCAAATG
  TAGAAAATGTCTTCCATCCAGTTGAGTGTTCCTACTGTCACAGTGAGAGCATGATGGGATTTCGATACCG
  ATGTCAACAGTGTCACAACTACCAGCTTTGCCAGGACTGCTTCTGGAGGGGCCATGCAGGAGGTTCTCAT
  AGCAACCAGCACCAAATGAAGGAGTATACGTCGTGGAAATCACCTGCTAAGAAGCTAACGAATGCATTAA
  GCAAGTCCCTGAGCTGTGCTTCAAGCCGTGAACCTTTGCACCCCATGTTCCCAGACCAGCCTGAAAAGCC
  ACTCAACTTGGCTCACATCGTTGATACTTGGCCTCCGAGACCTGTAACCAGCATGAACGACACACTCTTT
  TCCCACTCTGTTCCCTCCTCAGGAAGTCCTTTCATCACCAGGAGTATGCTTGAGAGTTCAAACCGGCTTG
  ATGAAGAACACAGGCTGATCGCCAGGTACGCCGCACGACTGGCAGCAGAATCCTCCTCATCACAGCCCAC
  ACAGCAGAGGAGTGCCCCTGACATCTCCTTCACCATCGATGCAAATAAACAGCAAAGGCAGCTGATTGCA
  GAGCTAGAGAACAAGAACAGAGAAATCTTACAAGAGATTCAGAGACTTCGGGTAGAACATGAGCAAGCTT
  CCCAGCCCACGCCAGAGAAAGCTCAGCAGAACCCAACCTTGCTGGCAGAACTCCGGCTCCTCAGACAGCG
  AAAGGATGAGCTAGAACAGAGAATGTCTGCTCTCCAGGAGAGCCGGAGAGAGCTGATGGTCCAGTTGGAA
  GGTCTCATGAAGCTCCTGAAGACCCAGGGGGCAAGCTCCCCACGCTCTTCCCCCAGCCACACCATCAGCA
  GGCCGATCCCCATGCCCATCCGTTCAGCATCTGCCTGTCCCACACCAACGCACACCCCGCAGGACTCCCT
  CACTGGGGTAGGCGGAGATGTGCAAGAGGCGTTTGCACAAAGTTCGAGAAGGAATCTAAGGAGTGACCTG
  CTGGTGGCTGCAGACTCCATCACCAATACCATGTCCTCTCTGGTGAAGGAGCTGAATTCAGAGGTGGCGA
  GTGAAACAGAAAGTACTGTGGATTCCGAGTTCTCGAGACCTCAGTTTGAGGATCTGGCTCCATCACCGAC
  CTCTGAGAAGGCTTTTCTAGCCCAAATCCACTCCCGGAAACCTGGGTACATTCACGGTGGAGCTGCAAGT
  ACCACGCATGGTGACATGGTCCCGGAAGATGGGGATCCTTACACGCAACCCGAGGATGGCAATTATGAGA
  ACGAGTCTGTGCGGCAGCTGGAGAATGAGCTGCAGCTGGAGGAGTACCTGAAGCAGAAGCTGCAGGACGA
  GGCCTACCAGGTCAGCTTGCAAGGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285810
• Insert Size: 2058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285810.1, NP_001272739.1
• RefSeq Size: 6219 bp
• RefSeq ORF: 2058 bp
• Locus ID: 13527
• UniProt ID: Q9D2N4
• Cytogenetics: 18 12.08 cM
• Gene Summary: Involved in synapse maturation and required for normal muscle function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR and has multiple differences in the coding region compared to variant 1. The resulting protein (isoform c) maintains the reading frame, and is shorter compared to isoform a. Variants 3 and 4 encode the same protein.