Dpysl3 (NM_001291455) Mouse Untagged Clone

CAT#: MC228666

Dpysl3 (untagged) - Mouse dihydropyrimidinase-like 3 (Dpysl3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dpysl3
• Synonyms: CRMP; CRMP-4; DRP-3; TUC4; U; Ul; Ulip; ULIP-1; Ulip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228666 representing NM_001291455
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGGGCCGAAGGGGTTGGGACAGCTCCCACGAGGACGACCTGCCTGTGTACTTGGCCCGTCCGG
  GCACCACAGATCAGGTCCCACGGCAGAAGTACGGTGGCATGTTCTGCAACGTGGAGGGTGCCTTCGAGAG
  CAAGACGTTGGATTTTGATGCCCTGAGTGTGGGACAGCGCGGCGCCAAGACTCCCCGGAGCAGCCAGGGC
  AGCGGCCGTGGCGCAGGGAACCGGCCCGGGATGGAAGTGGACGCGCGCAGGGGCCCGGGCCGGGAGGAGA
  GCAGGGAACCCGCGCCGGAGTCCCCCAAGCCCGCTGGGGTGGAGATCCGGAGCGCCACCGGCAAGGAGGT
  CTTGCAGAACCTTGGTCCCAAGGACAAGAGTGACCGTCTTCTAATCAAGGGAGGGAGAATCGTCAATGAT
  GATCAGTCCTTTTATGCTGATATTTACATGGAGGATGGCTTAATAAAGCAAATTGGAGACAATCTGATTG
  TCCCCGGAGGTGTGAAGACCATCGAGGCCAACGGGAAGATGGTGATCCCTGGAGGCATTGACGTCCATAC
  CCACTTCCAGATGCCTTACAAGGGAATGACCACAGTGGACGATTTCTTCCAAGGGACAAAGGCTGCCTTA
  GCGGGAGGCACCACCATGATCATTGACCACGTGGTACCTGAACCTGAGTCTAGCCTGACAGAGGCCTATG
  AAAAGTGGCGTGAGTGGGCCGATGGGAAGAGCTGCTGTGACTATGCTTTGCATGTGGACATCACCCACTG
  GAATGACAGCGTCAAGCAGGAGGTACAGAGCCTCAGCAAGGAAAAAGGCGTTAACTCCTTCATGGTTTAC
  ATGGCCTACAAGGATTTATATCAAGTGTCCAACACAGAGCTCTATGAGATCTTCACCTGCCTGGGAGAGC
  TGGGGGCCATTGCTCAAGTTCATGCCGAGAATGGAGATATCATTGCCCAGGAGCAGGCACGGATGCTGGA
  AATGGGGATAACCGGCCCAGAAGGCCACGTTCTGAGCAGACCGGAAGAGCTGGAAGCTGAGGCTGTGTTC
  CGTGCCATCACCGTCGCCAGCCAGACCAACTGCCCCCTCTATGTCACCAAGGTCATGAGCAAGAGCGCGG
  CTGATCTCATCTCACAAGCCAGGAAGAAAGGAAATGTGGTCTTTGGCGAGCCCATCACTGCCAGCCTGGG
  AATAGATGGAACTCATTACTGGAGTAAGAACTGGGCCAAAGCAGCTGCGTTTGTGACATCCCCACCTCTG
  AGCCCTGACCCTACCACACCTGACTACATCAACTCCTTGCTGGCCAGCGGGGATCTGCAGCTCTCTGGAA
  GTGCCCACTGTACCTTTAGCACTGCCCAGAAAGCCATTGGGAAGGACAACTTCACAGCCATCCCTGAAGG
  CACCAATGGCGTGGAGGAGCGTATGTCTGTCATCTGGGACAAGGCTGTGGCCACGGGGAAGATGGATGAA
  AACCAGTTTGTGGCCGTGACAAGCACCAATGCTGCCAAGATATTCAACCTGTACCCTCGAAAGGGGAGAA
  TAGCTGTGGGCTCTGACAGCGACCTTGTCATCTGGGATCCAGATGCCTTGAAGATTGTCTCTGCCAAGAA
  CCACCAGTCGGTTGCCGAATACAACATCTTTGAAGGGATGGAGCTGCGTGGTGCACCTCTGGTGGTTATC
  TGCCAGGGCAAGATCATGCTGGAAGATGGCAACCTGCACGTGACCCAGGGGGCTGGCCGCTTCATTCCCT
  GCAGCCCATTCTCTGACTATGTCTATAAGCGCATTAAAGCAAGGAGGAAGATGGCAGACCTGCATGCAGT
  CCCAAGAGGCATGTATGATGGACCAGTGTTTGACTTGACCACCACCCCCAAGGGGGGCACCCCAGCTGGC
  TCTACTCGGGGCTCTCCCACTCGGCCAAACCCGCCAGTGAGGAACCTCCATCAGTCAGGATTTAGCCTGT
  CAGGCACCCAAGTGGATGAGGGTGTCCGCTCAGCTAGCAAACGCATTGTGGCACCCCCTGGAGGCCGTTC
  TAACATCACATCCCTGAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291455
• Insert Size: 2052 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291455.1, NP_001278384.1
• RefSeq Size: 5502 bp
• RefSeq ORF: 2052 bp
• Locus ID: 22240
• Cytogenetics: 18 B3
• Gene Summary: This gene encodes a protein that belongs to the TUC (TOAD-64/Ulip/CRMP) family of proteins. Members of this family are phosphoproteins that function in axonal guidance and neuronal differentiation during development and regeneration of the nervous system. A mutation in the human gene is associated with amyotrophic lateral sclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (CRMP4b, also known as isoform 3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.