Zfp219 (NM_001253696) Mouse Untagged Clone

CAT#: MC228660

Zfp219 (untagged) - Mouse zinc finger protein 219 (Zfp219), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp219
• Synonyms: 2010302A17Rik; Znf219
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228660 representing NM_001253696
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCAGTGGGTTGGTCTGAGACTCGTGCAGGCGAACGGCGCTTCCCTTGTCCTGTGTGCGGAAAGC
  GCTTCCGATTCAATTCCATCTTGGCTTTGCACCTGAGAGCCCACCCGGGCGCCCAAGCCTTCCAGTGCCC
  ACATTGCGGCCACCGCGCAGCGCAGCGGGCTCTGCTGCGCTCACACCTCCGAACGCACCAGCCGGAGCGT
  CCACGCAGCCCTGCTGCACGGCTGTTGCTGGAGTTGGAGGAACGCGCCCTGCTTCGCGAAGCCCGACTCG
  GGAGAGCCAGAAGCTCAGGCGGCATGCAGTCCAGCCCTGCCGCAGAGGGCCTGGCTCGCCCTCAGGTCCC
  TTCCTCATCTGCCTTCCGTTGCCCTTTCTGCAAAGGCAAGTTTCGCACCTCAGCGGAGCGGGAACGCCAC
  CTGCATATCCTGCACAGGCCCTGGAAGTGCAGCTTGTGCAGTTTTGGCTCCAGCCAGGAGGAGGAGTTGC
  TGCACCACAGTCTGACGGCCCACGGGGCTTCCGAGCGCCCCCTGGCGGCTACATCTACGCCTGAACCCCC
  GCCTCCACCCCAGCAAGAACCCAGATCTGCCCTTGAGCCTGAGCCTGAGCCTGAACCTAGGCCAGAGCCT
  GACCGGGAGGCAAACCCTGCCCCAACTCCTGCACCTCCGGAGGAGCCCCCTGCGCCACCTGAATTCCGTT
  GCCAGGTGTGCGGCCAGAGCTTTACACAGTCCTGGTTTCTCAAGGGTCACATGCGCAAGCACAAGGCCTC
  CTTTGATCATGCGTGCCCCGTATGTGGTCGCTGCTTCAAGGAGCCCTGGTTCCTTAAGAACCACATGAAG
  GTGCACACCAGCAAGCTGGGTCCTTTGCGTGCTCCAGGCCCTGGCTCTGCACCTGCCAGGGCCCCTCAGC
  CTCCCGACCTGAGCCTACTGGCATATGAGCCACTGGGCCCTGCACTCCTCCTGGCCCCAGCACCCGCTCC
  GGCTGAGCGCCGAGAGCCCCCAAGCCTCTTAGGCTACCTGAGTGTACGAGCTGGGGAAGTACGACCCAAT
  GGTGAGGGTGCTGACCCTGGAGGTGGCCGAAGCTATGGAGGGTTCCGCCCACTGCCTTCAGCTCTTCCCA
  ACCGGGCTCGGCGACACCGTACAGAGGAACCAGAAGAGGAAGAAGAAGTGGTGGAGGCTGAAGAGGAGAG
  CTGGGCCCGAGGCAGGTCGCTGGGCTCTCTGACTTCCCTGCACCCCAACCCAGGTGAGGGGTCAGGACAG
  CCTGCACCTGCCGCGGGGACCCAGGCGAGATCCACGGCCACCCAAGAAGAAAACGGGCTGCTGGTTGGAG
  GGACACGATCTGAAGCGGGCCGTGGGGCCACTGGCAAGGACTGCCCCTTCTGTGGAAAATCTTTCCGCTC
  GGCGCATCACTTAAAAGTGCATCTCCGTGTGCACACAGGTGAGCGTCCCTACAAGTGTCCACACTGCGAC
  TATGCAGGTACCCAGTCGGGCTCGCTCAAGTATCACCTTCAGCGTCACCACCGAGAGCAGAGGAGCAGTG
  CGGGTCCTGGGCCTCCCCCAGAACCCCCGCCACCTTCCCAGCGGGGCTCACTGCAGCCGCAGTCAGGAGC
  CAAGCCAACTCAGGCCTCAGCCACCTGGGTAGAGGGCACTGCAAGTACCCGGCCTCCTTCGAGCAGCACC
  GGACCAGGGTCCCGTAGGAAGCCTGCTAGCCCTGGGAGGACCCTGCGAAACGGGCGAGGTGGTGAAGCCG
  AACCCCTGGACCTGTCCCTACGGGCGGGCCCGGGAGGTGAGGCCGGGGCAGGGGGTGCCCTTCACCGCTG
  CCTCTTCTGCCCCTTTGCCACTGGAGCTCCTGAGCTCATGGCCTTGCATCTGCAAGTACACCATAGCCGT
  CGTGCTCGGGGCCGCCGGCAGCCCCGAGCCGACACGTCTCCAACCTATGTCCGGGCACCATCAGGAGAGA
  CCCCTCCCAGTCCTCCACTAGAAGAGGAGGGCAGCCCAGGGCTGTCTAGATCCGGAGAGGCAGGTCTTGG
  GGGGCAAGAACGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253696
• Insert Size: 2046 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253696.1, NP_001240625.1
• RefSeq Size: 2844 bp
• RefSeq ORF: 2046 bp
• Locus ID: 69890
• Cytogenetics: 14 C2
• Gene Summary: Transcriptional regulator (PubMed:20940257). Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3' (PubMed:20940257). Binds to the HMGN1 promoter and may repress HMGN1 expression (By similarity). Regulates SNCA expression in primary cortical neurons (By similarity). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9 (PubMed:20940257). Plays a role in chondrocyte differentiation (PubMed:20940257).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an exon, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.