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Mid1 (NM_010797) Mouse Untagged Clone

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SKU
MC228655
Mid1 (untagged) - Mouse midline 1 (Mid1), transcript variant 1
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $686.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mid1
Synonyms 61B3-R; DXHXS1141; Fxy; Trim18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC228655 representing NM_010797
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC
CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC
TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA
GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATCATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC
CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCAACACCATGTCCTCTGCCGAGAAGGT
TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC
TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGGTGAATATGTACTG
TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA
GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTGGAGAGTAATCTCACCAATCTTATTAAGAGAA
ACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAAGTCAATGCATCCCG
TCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAATCATTCAGCAACGAAGACAAATTATT
GGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTTAGCTCAGCAGATTGCAAACTGTAAAC
AGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCACTCGCTGAAGGAAAATGACCACGCCCG
TTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTCCTAATT
CCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGATTTTTCCCGGGAGAAGAAACTGCTAG
AATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGAGAAGAGCTCTGCACCGCTTCCTACGA
CACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA
TTCACCGGACAAGCCAATGTTGTCAACGTGGCCTGTGACGGCACCTGTCTCCTCGGCTCCGCAGGTCTGT
GTAACTCGGCGGACAGCTGGATGATCGTGCCCAACATCAAGCAGAACCACTACACCGTGCACGGCCTGCA
AAGTGGCACCAAGTATATCTTCACGGTGAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGA
AAGCTGAAGACCAACAGTCAGCCGTTTAGACTGGATCCCAAATCGGCTCATCGCAAGCTGAAGGTGTCCC
ACGACAACCTGACTGTCGAGCGCGACGAGTCGTCCTCTAAGAAGAGTCACGCGCCGGAGCGCTTCGCTGG
TCAGGGGAGCTACGGAGTGGCTGGCAACGTGTTCATCGACAGCGGCCGTCACTACTGGGAAGTGGTCACC
AGCGGAAGCACATGGTACGCCATCGGCCTGGCGTACAGATCGGCGCCGAAACACGAGTGGATCGGGAAGA
ACGCGGCGTCCTGGGCCCTCTGCCGCTGCCACAACCACTGGGCGGTGCGACACGACGGCAAGGAGACCCC
CATCGCGCCGGCCCCTCACCTCAGGCGCGTCGGCGTCCTGCTGGACTACGACAACGGCTCCATCGCCTTC
TACGACGCTCTGAGCTCCGTCCACCTCCACACCTTCCACGCGGCGCTCGCGCAGCCCGTGTGCCCCACCT
TCACCGTGTGGAACAAGTGTCTGACCATCGTCACGGGTCTGCCCATCCCGGACCATCTGGACTGTACGGA
GCAGCGACCTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010797
Insert Size 2043 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010797.3, NP_034927.2
RefSeq Size 3860 bp
RefSeq ORF 2043 bp
Locus ID 17318
UniProt ID O70583
Cytogenetics X 79.19 cM
Summary Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_010797" in other vectors (1)
SKU Description Size Price
MR230866 Mid1 (myc-DDK-tagged) - Mouse midline 1 (Mid1), transcript variant 1 10 ug
$589.00 $623.00

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