Esrp1 (NM_001290383) Mouse Untagged Clone

CAT#: MC228654

Esrp1 (untagged) - Mouse epithelial splicing regulatory protein 1 (Esrp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Esrp1
• Synonyms: 2210008M09Rik; A630065D16; BC031468; Rbm35a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228654 representing NM_001290383
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGTCTCCGGATTACTTGGTGGTGCTTTTTGGGATCACTGCTGGGGCCACCGGAGCCAAGCTGG
  GTTCGGATGAGAAGGAGCTGATCCTGCTGTTATGGAAAGTCGTGGATTTGGCCAACAAGAAGGTGGGACA
  GCTGCACGAAGTACTAGTTAGACCGGATCAGTTGGAACTGACAGAGGATTGTAAAGAAGAAACTAAAATA
  GACGCCGAAAACCTGTCTTCCGCGCCGCAGCTGGACCAAGCCCTCCGACAGTTTAACCAGTCAGTGAGCA
  ATGAACTGAACATCGGGGTAGGGACTTCCTTCTGTCTCTGTACTGACGGGCAGCTTCACATTAGGCAGAT
  CTTGCATCCCGAGGCTTCCAAGAAGAATGTGTTACTGCCTGAGTGCTTCTATTCCTTTTTCGATCTTCGG
  AAAGAGTTCAAGAAGTGCTGCCCGGGCTCTCCCGATATCGACAAGCTGGATGTCGCAGCGATGACAGAAA
  GCCTGAACTTTGAGAAGAGTGACTCTGTGTCCCGATACGGAGCCTCCCAAGTTGAAGACATGGGGAACAT
  AATTTTAGCAATGATCTCCGAGCCCTACAATCACAGATTTTCAGATCCAGAGAGAGTAAACTACAAGTTT
  GAAAGTGGCACTTGCAAGATGGAACTTATTGATGACAGCACAGTGGTGAGAGCTCGAGGTTTGCCCTGGC
  AATCTTCAGATCAAGATATTGCAAGGTTCTTCAAAGGACTCAATATTGCCAAGGGTGGCGCAGCACTTTG
  CCTGAATGCTCAGGGCCGGAGGAACGGAGAAGCTCTGGTTAGATTTGTAAGTGAGGAGCACAGAGATCTA
  GCCCTGCAGAGGCACAAACATCACATGGGGACCCGGTACATTGAGGTTTACAAAGCAACAGGCGAAGATT
  TCCTTAAAATTGCCGGAGGGACATCCAATGAGGTAGCCCAGTTTCTCTCCAAGGAAAACCAAGTCATCGT
  GCGCATGAGGGGGCTCCCTTTCACGGCCACGGCTGAAGAAGTGGTGGCCTTCTTTGGACAGCACTGCCCC
  ATCACTGGAGGGAAGGAAGGCATCCTCTTCGTTACCTACCCAGATGGTAGGCCAACCGGAGATGCTTTTG
  TGCTCTTTGCTTGTGAGGAGTATGCTCAGAACGCCCTGAGGAAGCACAAGGAGTTGCTGGGTAAAAGATA
  CATCGAGCTCTTCAGGAGCACAGCTGCCGAAGTTCAGCAGGTGCTGAACCGATTCTCCTCAGCCCCTCTG
  ATTCCACTTCCAACCCCTCCCATTATCCCTGTACTACCTCAGCAGTTTGTGCCTCCCACGAATGTCAGAG
  ACTGCATACGTCTGCGAGGTCTTCCCTATGCTGCCACAATAGAAGACATTCTGGACTTCCTGGGGGAGTT
  TTCCACAGATATTCGTACACATGGAGTGCACATGGTATTGAATCACCAGGGACGCCCGTCAGGAGATGCC
  TTTATCCAGATGAAGTCAACAGACAGAGCATTCATGGCTGCGCAGAAGTACCATAAAAAAACCATGAAGG
  ACAGATATGTTGAAGTCTTTCAGTGTTCAGCTGAGGAGATGAACTTTGTGTTAATGGGGGGCACTTTAAA
  TCGAAATGGCTTGTCCCCACCGCCATGTAAGTTACCATGCCTGTCTCCTCCCTCCTACACATTCCCAGCT
  CCCACGGCTGTGATTCCTACCGAAGCTGCCATTTATCAACCTTCTCTGCTCTTGAACCCACGGGCACTGC
  AGCCCTCCACTGCATACTACCCGGCAGGCACTCAGCTCTTCATGAACTACACAGCCTACTATCCCAGCCC
  CCCAGGTTCGCCTAATAGTCTTGGCTACTTCCCTACAGCTGCTAATCTTAGCAGTGTCCCGCCCCAGCCT
  GGCACCGTGGTCAGAATGCAGGGCCTGGCCTACAATACTGGAGTTAAGGAAATTCTTAACTTCTTCCAAG
  GTTACCAGTATGCAACCGAGGATGGACTTGTACACACAAATGACCAGGCCAGGACTCTACCCAAAGAATG
  GGTTTGTATTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290383
• Insert Size: 2043 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290383.1, NP_001277312.1
• RefSeq Size: 3966 bp
• RefSeq ORF: 2043 bp
• Locus ID: 207920
• UniProt ID: Q3US41
• Cytogenetics: 4 A1
• Gene Summary: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (By similarity). Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (PubMed:29107558).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1.