Inpp4a (NM_001290798) Mouse Untagged Clone

CAT#: MC228650

Inpp4a (untagged) - Mouse inositol polyphosphate-4-phosphatase, type I (Inpp4a), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Inpp4a
• Synonyms: 107kDa; 9630012D15; D130048C09Rik; R74740
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228650 representing NM_001290798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTGTGTTTTGCTTGCAGGGTATGTGAGTTGGAAGAGTTGGGGGAGCTGTCCCCTTGCTGGGAGA
  GCCTCCGGCGCCAGATTGTCACCCAGTATCAGACTATCATCCTCACCTACCAGGAGAATCTGACTGACCT
  CCATCAGTACAAAGGTCCTTCCTTTAAAGCAAGCAGTTTGAAAGCAGATAAAAAGTTAGAATTTGTTCCC
  ACAAACCTGCACATACAACGGATGCGAGTTCAAGATGATGGAGGCTCAGATCAGAACTACGATGTCGTCA
  CTATTGGAGCCCCAGCAGCACACTGCCAAGGTTTTAAGTCAGGAGGTCTTCGAAAAAAGCTGCACAAGTT
  TGAAGAGGCCAAGAAACACAGTTTTGAGGAGTGTTGTACATCTTCTAGTGGCCAGTCCATCATCTACATA
  CCACAGGACATCACCCGAGCCAAGGAGATCATTGCCCAGATCAACACCCTAAAAACCCAGGTCAGCTACT
  ACGCGGAACGGCTCTCAAGGGCTGCGAAGGACAGGTCAGCTACTGGCCTTGAGAGGACTCTCGCCATCTT
  GGCAGACAAGACTCGGCAGTTGGTGACTGTCTGTGACTGTAAGCTGCTGGCCAACTCCATCCATGGGCTG
  AATGCAGCTCGGCCCGACTACATCGCTTCCAAGGCCTCCCCTACCTCGACTGAGGAGGAGCAGGTGATGC
  TTCGGAATGACCAGGACACCCTGATGGCCAGGTGGGCGGGGAGAAGCAGCCGGTCTTCCCTGCAGGTGGA
  CTGGCATGAGGAGGAGTGGGAGAAAGTGTGGCTGAATGTGGACAAGAGCCTGGAGTGCATCATTCAGCGG
  GTGGACAAGCTGCTGCAGAAGGAGCGTCTGCATGGGGAGGGCTGCGAGGATGCTTTCCCCTGTTCGAGCA
  CCTGCTCCAGCAAGAAAGGTGAGTGGAGCGAGGCCCTTTACCCTCTGCTGACCACCCTCACAGACTGTGT
  GGCCATGATGAGCGACAAGGCCAAGGCAGCCATGGTCTTCCTGCTCATGCAGGACAGTGCCCCCACCATT
  GCCTCGTACCTCAGCCTGCAGTATCGCCGTGACGTCGTCTTCTGCCAAACCCTGACAGCCCTCATCTGTG
  GCTTTATCATCAAGCTGAGGAACTGCCTGCACGACGGTGGCTTCCTGCGGCAGCTCTACACCATCGGCCT
  TCTGGCCCAGTTTGAGAGCCTGCTGAGCACCTACGGAGAGGAGTTGGCCATGTTGGAGGACATGAGCCTT
  GGAATCATGGACCTGAGGAATGTGACCTTTAAAGTCACTCAGGCCACTTCGAATGCATCAAATGACATGC
  TGCCGGTCATCACAGGAAACCGGGATGGCTTTAACGTGCGGATCCCTCTGCCAGGCCCACTGTTTGACTC
  CCTCCCCAGAGAGATCCAGAGTGGCATGCTGCTGCGGGTGCAGCCTGTCCTCTTCAACGTGGGCATCAAT
  GAGCAACAGACACTGGCTGAGAGGTTTGGAGATACGTCCTTACAAGAAGTTATCAATGTGGAGAGCTTGG
  TGCGGTTAAATTCCTACTTTGAGCAGTTTAAGGAGGTTTTGCCGGAGGACTGTCTACCTCGATCTCGGAG
  TCAGACCTGCCTTCCAGAGCTGCTGCGGTTCCTGGGACAGAATGTCCATGCACGCAAGAATAAGAATGTG
  GACATCCTCTGGCAAGCTGCTGAGGTCTGTCGCCGCCTTAACGGGGTCCGATTCACCAGCTGCAAGAGTG
  CCAAGGACCGCACAGCCATGTCGGTGACCCTGGAGCAGTGTCTGATCCTGCAGCACGAGCACGGCATGGC
  CCCGCAGGTCTTCACACAAGCCCTGGAGTGCATGCGCAGCATTGGAACACGGGAGGTAGTCACCCAGAAG
  AACTTGAGCGGCCTGGTGCCCATCCGAGACTTAAGGCTAGACCCCAGCCTTCTCTGTTCCATTCCTTTAT
  TAGCTCTAAGCCCCAATCTACTGATTGTGTGGCTCTTTCTGAGCATAGCGTACCTGGTGACCAAATTGCG
  TTGCAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290798
• Insert Size: 2040 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290798.1, NP_001277727.1
• RefSeq Size: 2971 bp
• RefSeq ORF: 2040 bp
• Locus ID: 269180
• UniProt ID: Q9EPW0
• Cytogenetics: 1 15.46 cM
• Gene Summary: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate. Involved in the regulation of megakaryocyte and fibroblast proliferation. Regulates cell growth downstream of transcription factor GATA-1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame, compared to variant 3. These differences include an alternate translation initiation start codon and an alternate 3' terminal exon. The encoded protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 3.