Sgsm1 (NM_001254731) Mouse Untagged Clone

CAT#: MC228644

Sgsm1 (untagged) - Mouse small G protein signaling modulator 1 (Sgsm1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgsm1
• Synonyms: 2410098H20Rik; AA408781; D5Bwg1524e; E130107M15; NuIP; Rutbc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228644 representing NM_001254731
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGGTCCCTGCGGAGGCCGAGACCCGCCAGAGGCTGCTGCGCACTGTGAAGAAGGAGGTGAAGC
  AGATCATGGAAGAAGCCGTTACCAGAAAGTTCGTCCACGAGGACAGCAGCCACATCATCTCCTTCTGTGC
  GGCCGTGGAGGCCTGCGTGCTGCACGGGCTGCGGCGGAGGGCGGCCGGCTTCCTGCGAAGCAACAAGATC
  GCGGCGCTCTTCATGAAGGTGGGCAAAGGCTTCCCTCCGGCCGAGGAGCTGAGCCGCAAGGTCCAGGAAC
  TGGAGCAACTCATTGAGAGCGCGCGAAACCAGATCCAGGGCCTGCAAGAGAACGTGCGGAAGCTGCCGAA
  GCTGCCTAACCTGTCCCCGCTGGCCATCAAGCACCTGTGGATCCGCACCGCCCTGTTCGAGAGGGTCCTG
  GACAAAATCGTCCACTACCTGGTGGAAAACAGCAGTAAATACTACGAGAAGGAGGCACTCTTGATGGACC
  CGGTGGACGGACCCATCCTGGCCTCTTTGTTGGTGGGGCCATGTGCCCTGGAGTATACCAAGATGAAGAC
  TGCAGATCACTTCTGGACTGACCCCTCAGCCGACGAGCTGGTCCAGAGACACCGGATCCACAGCTCACAT
  CTGAGACAGGACTCGCCCACCAAGCGGCCAGCGCTCTGTATCCAGAAGAGGCATTCCAGTGGCAGCATGG
  ACGACCGGCCATCTATCTCTGCCCGGGACTATGTAGAATCTCTGCACCAGAACTCCAGGGCCACCCTGCT
  CTATGGAAAGAACAATGTTCTGGTTCAGCCAAGGGATGACATGGAGGCCGTGCCAGGGTACCTGTCTCTG
  CACCAGACAGCGGATGTCATGACCTTGAAGTGGACACCCAATCAGCTGATGAACGGGTCTGTGGGGGATC
  TGGACTACGAGAAGAGTGTCTACTGGGACTACGCTGTGACCATCCGCTTAGAGGAGATAGTTTACCTGCA
  CTGCCATCAACAAGTGGACAGCGGCGGGACTGTCGTGCTGGTGAGCCAGGATGGAATCCAGAGGCCACCC
  TTCCGCTTCCCCAAGGGCGGGCACCTGCTACAGTTCCTCTCCTGTCTGGAGAATGGGCTGCTTCCGCACG
  GGCAGCTGGACCCGCCGCTTTGGTCACAGCGAGGAAAGGGGAAGGTATTTCCTAAGTTGCGCAAGCGCAG
  CCCACAGGGGTCCTCCGAGTCCACGTCTTCAGACAAGGAGGACGACGAAGCCACGGATTACGTGTTCCGC
  ATCATCTACCCTGGCACGCAGTCTGAATTCGTGCCCCAGGACCTAATGGATGTCTCTATGAACAACCTTC
  CACCCCTATGGCAACCCAGTCCTCGGAAGTCCTCCTGCTCTTCCTGTTCACAAAGCGGCTCAGCTGACGG
  CGGCTCAACCAATGGCTGTAACCATGAGAGGGCCCCACTGAAACTGCTGTGTGATAACATGAAGTACCAG
  ATCCTCTCCAGAGCCTTCTATGGATGGCTCGCCTACTGCAGACACCTGTCCACCGTGAGGACCCACCTGT
  CAGCCCTGGTCAATCACATGATCGTGTCTCCAGACCTGCCCTGTGACGCTGGGCAAGGGTTGACAGCCAG
  CATCTGGGAAAAATACATCCAGGACAGCACGACCTACCCGGAGCAGGAGCTGCTGCGTCTCATCTACTAT
  GGAGGTGTCCAGCCTGAGATCCGCAGGGCAGTGTGGCCCTTCCTCCTCGGCCACTACCAGTTTGGGATGA
  CAGAGATGGAGAGGAAAGAGGTAGACGAACAGATCCATGCCTGCTACGCACAAACCATGTCGGAGTGGCT
  GGGCTGTGAAGCTATCGTGAGACAGAGGGAGCGGGAGTCCCACGCGGCTGCCCTGGCCAAGTGCTCTTCA
  GGAGCCAGCCTGGACAGCCACCTTCACCGGATGCTGCACCGGGACTCCACCATCAGCAATGAGTCATCCC
  AGAGCTGCAGCTCAGGCCGCCAGAACCTCCGACTGCAGAGCGACTCCAGCAGCAGCACACAGGTGACTTT
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001254731
• Insert Size: 2034 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001254731.1, NP_001241660.1
• RefSeq Size: 2335 bp
• RefSeq ORF: 2034 bp
• Locus ID: 52850
• Cytogenetics: 5 55.45 cM
• Gene Summary: Interacts with numerous Rab family members, functioning as Rab effector for some, and as GTPase activator for others. Promotes GTP hydrolysis by RAB34 and RAB36. Probably functions as GTPase effector with RAB9A and RAB9B; does not stimulate GTP hydrolysis with RAB9A and RAB9B.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 3' UTR and has multiple coding region differences, compared to variant 1, one of which results in a frameshift. The resulting protein (isoform c) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.