Adam32 (NM_001293693) Mouse Untagged Clone

CAT#: MC228643

Adam32 (untagged) - Mouse a disintegrin and metallopeptidase domain 32 (Adam32), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228643 representing NM_001293693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCGCCATGCTGCACACACTGCTGCTGCTGCTGCTGGCCGAGCTGGGGGCCTTGCTGGCATCAG
  GACCCGAGTCTCAAAGTTCATTTCTAGAAATCATATTTCCAGAGAAAATTGAAGATAAAACACACTCAGA
  AGAACAGATATCCTATATTATTCCAATAAACAAGAAGCAGTACACTGTGCACCTCCAGAAAAGATATTTT
  TTAACGAATCGTTTTATGGTTTATATGTATAATCAAGGATCTACAAGTTTTCATTCTCCAAACATTCCGG
  CTCAGTGCTATTATCAAGGACACATCAAAGGCTACCCGAACTCAGTCGCCACCCTCAGCACATGCTCTGG
  ACTGAGAGGGTTCCTGCAGTTTGAGAATGTGTCCTACGGAATTGAGCCTCTGCAATCTGCATTTACATCT
  CAACACATTGTTTATAAGCTAGGGAACAAAGAGAAGGAGTTGATATTTAACAAAAACAGCAGAAACATAG
  AGATGCCTACAAACTATGGCATTTTAATCAACAAAAAGCCAAAATCACCTTTTAAAAACTTGTTTCCCCT
  CTATCTAGAGATGAGTATTGTGGTGGACAAAGCGCTGTATGATTACTTGGGCTCTGACAGCAATATTGTA
  ACAAATAAAATCATTGAAATTATCAGTCTTATAAATTCAGTGTTTGCCCAACTCAAGGTTACTATCGTGT
  TGTCCTCATTGGAGTTGTGGTCAGATAAGAATAAGATTCCTACAGTTGGTGAAGCAGATGAATTATTGCA
  TAAATTTCTAGAATGGAAGCAGGCCTACCTTACCCTAAGACCTCATGATGTTGCATATCTATTCATTTAT
  AACGAGTACCCAAATTACATGGGAGCAACGTATCCTGGGAAGATGTGTACTGCCCACTACTCTGCAGGAA
  TCACAATGTACCCCAAGGATATGACCTTGGAGGCCTTTTCGGTTATTCTCACCCAAATGCTGGGGCTCAG
  TCTGGGGATATCGTATGATGAGCCTGAGAAATGCTACTGCTCGGAATCCATCTGCATCATGAATCCCAGG
  GCGATGCAATATGGAGGTGTGAAGTCATTTAGCAATTGCAGTTTGAATGACTTTGAACATTTTAAATCGA
  ACGAGGGTGCCAAATGTCTGCAGAATAAGCCACAGATGCAAAGGACTGCAGCAGCGGTCTGCGGAAATGG
  CAAGGTGGAGGGTGATGAAATTTGTGACTGTGGCTCTGAAGCAGAATGTGGGCCTGATAGCTGTTGTGAA
  CCTAACAGATGTGTTCTGAAAGCAGGAAGAGCTTGTGACAGTAAGTCTCCCTCATCAACATGCTGCAAAA
  ATTGTCAATTTTTACCAGAAAAACATCAATGTAGGCCGGAAAAGCATCTATACTGTGACATTCCTGAAGT
  CTGCAATGGGAGCTCAGGAAATTGTCCACCTGATGTAACTATAAACAATGGGCATGTTTGCAAAGAAAGC
  GGGACTATCTGTTATAATGGAGACTGTCCGGACCTGGACAGGGTGTGCGAGTCAATATATGGAGCAGGTT
  CAGTGAATGCTCCATTTGCCTGCTATGAAGAAATCCAGGGCCAAAATGATAGGTTTGGCAACTGTGGCAA
  AGACAACCGAAACAGATATGTGTTCTGTGGATGGAGGAATCTCATATGTGGGAGATTGATTTGTACGTAC
  CCTACCCGAATGCCTTACAACCCACCCAATAACAGTACAGCTTCTGTGATCTATGCTTTTGTGCGAGACA
  AAGTGTGTATAACTGTGGACTTTGGATCGAGTGTCAAAGAAGATCCACTCAGGGTTGCTAATGGTGCTAC
  ATGTGATCTTGATAGGATCTGCTTAAATGGCGTGTGCGTAGAATCGAGATTTCTTAGGGATCAATCAAAA
  ACGTGTTCATCCAAGTGCCACGGGAACGGAAAGCAAATCAGAAGACAGCACTTACACGTATGTCAGCAGG
  TGAGTTCATTAAAAGACGGTGTGAAGAGACATGTTGAAAATGAACATCATAACTTTCAGCCGATCAACTT
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293693
• Insert Size: 2034 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293693.1, NP_001280622.1
• RefSeq Size: 2247 bp
• RefSeq ORF: 2034 bp
• Locus ID: 353188
• UniProt ID: Q8K410
• Cytogenetics: 8 A2
• Gene Summary: This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene was found to be expressed predominantly in the pachytene spermatocytes, where the processed protein is localized to the sperm surface. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) lacks two coding exons and uses an alternate splice junction at the 3' end of a coding exon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1.