Brsk2 (NM_001276763) Mouse Untagged Clone

CAT#: MC228642

Brsk2 (untagged) - Mouse BR serine/threonine kinase 2 (Brsk2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brsk2
• Synonyms: 4833424K13Rik; SAD-A; SADA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228642 representing NM_001276763
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCGACGGGGAAGGACGGCGGCGGGGCGCAGCACGCGCAGTATGTGGGGCCCTACCGGCTGGAGA
  AGACGCTGGGCAAGGGGCAGACAGGCTTGGTGAAGCTGGGAATCCACTGTGTCACTTGCCAGAAGGTCGC
  CATCAAAATCGTGAACCGTGAGAAGCTCAGTGAGTCGGTGCTGATGAAGGTGGAGCGAGAGATTGCCATC
  TTGAAGCTCATCGAGCATCCACATGTACTGAAGCTGCATGACGTCTATGAAAACAAAAAATATTTATACC
  TGGTGCTAGAACATGTGTCTGGGGGAGAGCTGTTCGACTACCTGGTGAAGAAGGGCCGGCTGACCCCCAA
  GGAGGCCCGCAAGTTCTTCCGGCAGATCATCTCTGCACTGGACTTCTGTCACAGCCACTCCATATGCCAT
  AGAGACTTGAAGCCAGAGAACCTGCTGCTAGATGAGAGGAACAACATCCGTATTGCAGACTTTGGCATGG
  CATCCCTGCAGGTGGGAGACAGCCTGCTGGAGACCAGCTGCGGATCTCCACACTATGCCTGTCCGGAAGT
  GATTCGGGGCGAGAAGTATGATGGCCGCAAGGCAGATGTGTGGAGCTGTGGTGTGATCCTGTTCGCCTTG
  CTGGTGGGGGCTCTGCCTTTTGATGATGACAACCTGCGGCAGTTGCTGGAGAAGGTCAAGCGTGGTGTGT
  TCCACATGCCACACTTTATCCCACCAGACTGCCAGAGTCTCCTGCGTGGCATGATTGAGGTGGATGCAGC
  TCGGCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGTGGCAAGAATGAGCCAGAGCCC
  GAACAGCCCATCCCACGCAAGGTGCAGATCCGCTCACTACCCAGCTTGGAAGACATTGACCCTGATGTGT
  TGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGATCTGCTATCTGAGGA
  GGAGAATCAGGAAAAGATGATTTATTTCCTCCTCCTGGATCGGAAAGAACGGTATCCAAGCCATGAGGAT
  GAGGACCTGCCCCCCAGGAATGAGATAGACCCTCCCCGGAAGCGTGTGGATTCCCCGATGCTGAACCGGC
  ATGGCAAGCGGCGACCTGAGCGCAAGTCCATGGAAGTGCTCAGTGTGACAGATGGTGGCTCCCCAGTGCC
  TGCACGGAGAGCCATTGAGATGGCCCAGCATGGCCAGAGTAAAGCAGTGTTCAGTAAAAGCCTGGATATC
  GCTGAAGCCCATCCCCAATTCAGCAAAGAAGACAGATCTCGATCCATCAGTGGTGCGTCCTCAGGCCTTT
  CTACAAGTCCACTCAGCAGTCCTCGGGTGACCCCTCACCCCTCACCAAGGGGTAGTCCCCTTCCTACCCC
  CAAAGGGACGCCTGTCCACACGCCAAAGGAGAGCCCAGCTGGCACACCCAACCCCACACCACCATCCAGC
  CCTAGTGTTGGAGGAGTGCCCTGGCGGACACGACTGAACTCCATCAAGAACAGCTTCCTGGGCTCACCTC
  GATTCCACCGCCGGAAACTCCAAGTTCCCACGCCAGAGGAGATGTCCAACCTGACCCCAGAATCCTCTCC
  AGAGCTGGCCAAGAAATCGTGGTTCGGGAACTTCATCAACCTGGAGAAGGAGGAGCAGATCTTTGTGGTG
  ATCAAGGACAAGCCCCTGAGCTCCATCAAGGCTGACATCGTTCATGCCTTCCTGTCGATCCCCAGCCTCA
  GCCACAGCGTTATTTCCCAGACAAGCTTCAGGGCTGAATACAAGGCCACAGGGGGCCCAGCAGTGTTCCA
  GAAGCCGGTCAAGTTCCAGGTGGACATCACCTACACTGAGGGCGGAGAGGCCCAGAAGGAGAATGGCATC
  TACTCAGTCACATTCACTTTACTCTCAGGCCCCAGTCGCCGCTTCAAGAGGGTGGTGGAGACCATCCAGG
  CCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGGAATTATCCCGAAAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276763
• Insert Size: 2028 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276763.1, NP_001263692.1
• RefSeq Size: 4113 bp
• RefSeq ORF: 2028 bp
• Locus ID: 75770
• Cytogenetics: 7 F5
• Gene Summary: Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has multiple differences in the coding region and 3' UTR, compared to variant 3. The resulting protein (isoform delta) is shorter than isoform gamma. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.