Gpcpd1 (NM_028802) Mouse Untagged Clone

CAT#: MC228641

Gpcpd1 (untagged) - Mouse glycerophosphocholine phosphodiesterase GDE1 homolog (S. cerevisiae) (Gpcpd1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpcpd1
• Synonyms: 2310004G06Rik; 2310032D16Rik; AU015213; Gde5; mKIAA1434; Prei4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228641 representing NM_028802
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACCTTCTCAGGTCACTTTTGAAATAAGAGGAACTCTTTTACCAGGAGAGGTCTTTGCAATATGTG
  GAAGCTGTGATGCCCTGGGAAACTGGAATCCTCAAAATGCTGTGGCTCTTATTAATGAAAACGAGACAGG
  AGACAGTGTGTTGTGGAAAGCAGTGATTGCTCTCAATAGAGGAGTGTCAGTGAAGTACCGCTACTTCAGA
  GGCTGCTTTTTAGAACCAAAGACTATCGGTGGTCCATGTCAAGTCATAGTTCACAAGTGGGAGACTCATC
  TACAACCACGATCAATAACCCCTTTAGAAAGTGAAATCATTATTGACGATGGACAGTTTGGCATCCACAA
  TGGTGTTGAAACACTGGATTCTGGATGGCTTACATGTCAGACTGAAATAAGATTGCGTCTGCATTTTTCT
  GAGAAACCTCCTGTTTCAATTAGCAAGAAAAAGTTCAAAAAATCGAGATTTAGGGTAAAGCTCACACTCG
  AGGGTCTGGAGGAAGATGAAGATGATGATGACGATAAGGTCTCTCCCACTGTTCTTCACAAAATGTCCAA
  CAGCCTGGAGATATCCTTAATAAGTGACAATGAGTTCAAGTGCAGGCACTCACAGCCAGAATGTGGGTAT
  GGCTTACAGCCCGATCGTTGGACAGAGTACAGCATACAGACAATGGAACCAGATAATCTGGAGCTCATCT
  TTGACTTTTTTGAGGAAGATCTCAGTGAGCATGTAGTTCAGGGTGATGTTCTTCCTGGACACGTGGGCAC
  AGCATGCCTCCTGTCTTCTACCATTGCTGAGAGTGGAAGAAGCGCTGGAATCCTTACTCTTCCCATCATG
  AGCAGAAATTCCAGAAAAACTATAGGCAAAGTCAGAGTTGATTTTATCATCATCAAGCCATTACCTGGAT
  ATAGTTGTTCTATGCAGTCTTCATTTTCCAAGTATTGGAAACCAAGAATACCATTGGACGTTGGACATCG
  TGGTGCAGGGAACTCAACAACGACTGCCAAGCTAGCTAAAGTACAGGAAAATACTATCGCTTCTTTAAGA
  AATGCTGCCAGTCATGGCGCAGCATTTGTAGAATTTGATGTCCACCTTTCAAAGGACTTTGTGCCCGTGG
  TGTATCATGACCTCACCTGCTGTCTGACCATGAAGAGGAAATATGAAGCTGATCCAGTTGAATTGTTTGA
  AATCCCAGTAAAAGAATTAACATTTGACCAACTCCAGTTATTGAAGCTTTCTCATGTGACTGCATTAAAA
  ACCAAAGACCGGAAACAATCTTTGTATGAGGAGGAAAATTTCTTTTCTGAAAATCAGCCATTTCCTTCTC
  TTAAGATGGTTTTAGAATCATTGCCAGAAAATGTAGGATTTAATATAGAAATAAAATGGATTTGCCAACA
  CAGGGATGGAGTATGGGATGGCAACTTATCAACATATTTTGATATGAATGTGTTTTTGGATATAATTTTA
  AAAACTGTTTTAGAAAATTCTGGGAAGAGGAGAATAGTGTTTTCTTCTTTTGATGCAGATATTTGTACAA
  TGGTTCGGCAGAAGCAGAACAAATATCCCATATTATTTTTGACCCAAGGAAAGTCTGATATTTACCCCGA
  ACTCATGGACCTCAGATCTCGGACAACACCCATTGCAATGAGTTTTGCACAGTTTGAAAATATTTTGGGG
  ATAAATGCCCATACTGAAGACCTCCTTAGAAACCCATCCTATGTCCAAGAGGCAAAAGCTAAGGGATTGG
  TCATATTCTGCTGGGGTGATGATACCAACGATCCTGAAAACAGAAGGAAACTGAAGGAATTTGGAGTAAA
  TGGTCTAATATATGATAGGATATATGATTGGATGCCTGAACAGCCAAATATATTCCAAGTGGAGCAGTTG
  GAGCGCCTGAAGCAAGAATTGCCAGAGCTTAAGAACTGTTTGTGTCCCACTGTTAGCCACTTCATCCCTT
  CTTCTTTCTGTGTGGAGCCTGATATCCACGTGGATGCCAACGGCATTGATAGTGTGGAGAACGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028802
• Insert Size: 2028 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028802.3, NP_083078.3
• RefSeq Size: 3716 bp
• RefSeq ORF: 2028 bp
• Locus ID: 74182
• UniProt ID: Q8C0L9
• Cytogenetics: 2
• Gene Summary: May be involved in the negative regulation of skeletal muscle differentiation, independently of its glycerophosphocholine phosphodiesterase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 8. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 4. Variants 2 and 10 both encode the same isoform (2).