Zscan10 (NM_001289482) Mouse Untagged Clone

CAT#: MC228635

Zscan10 (untagged) - Mouse zinc finger and SCAN domain containing 10 (Zscan10), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zscan10
• Synonyms: BC068284; Zfp206; Zkscan10; Znf206
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228635 representing NM_001289482
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCGGAACCAGTCCCTGATGCCCTGGAACAAGAGCATCCCGGAGCAGTGAAGTTGGAGGAGGATG
  AAGTTGGCGAGGAGGATCCCAGGCTCGCAGAGTCCAGGCCTAGGCCTGAGGTGGCCCACCAGCTTTTCAG
  ATGCTTCCAGTATCAGGAAGATATGGGGCCACGGGCATCCCTGGGCCGGCTCCGGGAACTCTGCAACCAC
  TGGCTGCGACCGGCTCTGCACACCAAGAAGCAGATCCTGGAGCTGCTGGTACTGGAGCAGTTCCTGAGTG
  TCCTGCCCCCGCATGTGCTGAGCCGGCTGCACGGCCAACCGCTCCGGGACGGAGAGGAGGTGGTACAGCT
  ATTGGAGGGCGTGCCCAGAGACATCAGCCACATGGGGCCACTGGAGTCCCTCACCTTTGAGGATACCTCA
  GAGAAGAGAGCTTGGCCTGCAAACCCTCTTGGATTTGGAAGCAGAATGCCTGACAATGAGGAACTTAAAG
  TTGAAGAGCCTAAAGTGACTACTTGGCCTGTCGTCATTGGAGCAGAGTCCCAGACAGAGAAACCTGAAGT
  TGCAGGAGAGCCTCTTACGCAAACTGTAGGGCAGGAGACCAGCAGCACTGGTTGGGGAGGTACTCCTGCT
  GACGGCAGTGAAGTTGTGAAGGTTAGAGGAGCTTCCGATGCCCCAGAGCCCCAGGGGGAGATGCAGTTCA
  TATGTACATATTGTGGGGTAAACTTCCCAGAGATGTCTCATCTACAGGCCCACCAGTTACAATCTCACCC
  CAACTTGCAACCTCACCCAAGCTCTCGATCCTTCCGATGTCTGTGGTGTGGGAAGACTTTTGGACGCAGC
  TCGATCCTCAAGCTGCACATGCGCACTCACACAGACGAGCGGCCGCACGCCTGTCATCTCTGCAACCGCC
  GCTTCCGCCAGAGCTCACACCTGACGAAGCACTTGCTAACGCATTCCTCTGAGCCTGCCTTCCGATGCGC
  CGAGTGTAACCAGGGTTTTCAGCGTCGCTCCAGCCTCATGCAGCACCTGCTGGCACATGCCCAGGGAAAG
  AATCTCACGCCAAATCCAGAAGGCAAGACAAAAGTGCCAGAGATGGCAGCTGTCCTCTGTTCCCACTGCG
  GGCAGACCTTCAAGCGGCGCTCTAGCTTAAAGCGTCACCTGCGTAACCATGCCAAGGACAAGGACCATCT
  GTCCTCTGAAGACCCTGGCAGCCTTAGCTCTAGCCAGGAGAGTAACCCCTATGTGTGTAGTGACTGTGGC
  AAGGCCTTCCGACAAAGCGAGCAACTAATGATCCACACTAGGCGAGTCCATACCCGTGAACGACCCTTCT
  CCTGCCAGGTCTGTGGCCGCTGCTTTACCCAAAATTCCCAGCTGATCAGCCACCAGCAGATTCATACGGG
  TGAGAAGCCTCACGCCTGTCCTCAGTGCAGCAAACGCTTTGTGAGACGAGCTGGCCTTGCTCGGCATCTG
  TTGACCCACGGTAGCCTCCGGCCTTACCACTGTGCCCAATGTGGCAAAAGCTTTCGCCAAATGCGAGACC
  TAACCCGCCACGTACGCTGCCACACGGGGGAGAAGCCCTGCCGATGCAACGAATGTGGAGAGGGGTTCAC
  CCAGAATGCCCACCTGGCACGCCACCAACGCATCCACACGGGGGAGAAGCCCCACGCCTGTGACATCTGT
  GGTCACCGCTTTCGTAACAGCTCCAACTTGGCCCGCCACCGCCGCAGCCACACTGGCGAACGGCCCTATA
  GCTGTCCAACCTGTGGCCGCAGTTTCCGGCGCAATGCGCACCTGCAGCGCCACCTGATCACACACACAGG
  GTCAAAGCAAGAAAAGGAAGTTCCTCAGGAGTGCCCTGAGTGTGGCAAGAGCTTCAATCGCAGCTGCAAC
  TTGCTGCGCCACCTGCTGGTTCACACCGGTGCAAGGCCTTACTCCTGTGCACTGTGTGGCCGCAGCTTCA
  GCCGTAATTCACACCTGCTGCGCCACCTGCGAACCCATGCCCGGGAATCGCTGTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289482
• Insert Size: 2019 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289482.1, NP_001276411.1
• RefSeq Size: 2405 bp
• RefSeq ORF: 2019 bp
• Locus ID: 332221
• UniProt ID: Q3URR7
• Cytogenetics: 17 A3.3
• Gene Summary: Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency. Can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons in the coding region, compared to variant 1. This results in an isoform (3) that is shorter, compared to isoform 1.