Kif2c (NM_001290662) Mouse Untagged Clone

CAT#: MC228631

Kif2c (untagged) - Mouse kinesin family member 2C (Kif2c), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif2c
• Synonyms: 4930402F02Rik; ESTM5; Knsl6; MCAK; X83316
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228631 representing NM_001290662
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGACATTGACGATGTGGCTGCAATAAACCCAGAGCTCCTACAGCTTCTTCCCTTACGACCGAAGG
  ACAGTCTGCCACTGCAGGAAAACGTAACGGTCCCGAAGCAAAAACGCAAATCAGTCAACTCCAAAATTCC
  AGCTCTAAAAGAAGGCCTTCGAAGCCGCTCCACTCGCATGTCCACTGTCTCAGAAGTTCGAATCCCTGCT
  CAGGAGAATGAAATGGAGGTGGAGCTGCCTGTGCCTACAAACTCTCGCAAGCAGTTTGCCATTCCCAGCC
  ACCCTCGGGCCTCCTGCTCCACAGTGACAGAATTGCCGTTGTTGATGGTCAGTGAGGAGGCAGAAGAGCA
  AGCCCACTCCACCCGCAGTACCTCATCTGCAAACCCTGGGAACTCAGTTCGGAGGAAATCGTGTATCGTG
  AAGGAGATGGAGAAAATGAAGAACAAGCGTGAAGAGAAGAGGGCCCAGAACTCGGAGCTAAGAATAAAGC
  GAGCACAGGAATATGACAGCAGCTTTCCAAACTGGGAATTTGCCCGGATGATCAAAGAATTTCGGGTTAC
  TATGGAATGTAGCCCGCTTACTGTGACAGACCCTATCGAAGAGCACAGGATCTGTGTCTGTGTCAGGAAA
  CGCCCACTGAATAAACAAGAACTGGCCAAGAAAGAAATCGATGTGATTTCTGTTCCCAGCAAGTGTCTCC
  TCCTGGTGCATGAACCCAAGTTAAAAGTGGACTTAACGAAGTACCTGGAGAACCAAGCTTTCTGCTTTGA
  CTTTGCATTTGATGAGACAGCTTCCAATGAAGTTGTCTACAGGTTCACAGCAAGGCCACTGGTACAGACG
  ATTTTTGAAGGGGGAAAAGCAACATGCTTTGCCTATGGACAGACAGGCAGTGGGAAGACACATACAATGG
  GTGGAGATCTGTCTGGTAAAAGTCAGAACGCATCTAAAGGGATCTACGCCATGGCTTCCCGGGATGTCTT
  CCTTCTGAAGAACCAGCCTCGGTACCGGAACTTAAACCTGGAAGTTTATGTGACATTCTTCGAGATCTAC
  AATGGGAAGGTGTTTGATCTGCTCAACAAAAAGGCCAAGTTACGTGTGCTGGAGGACAGCAGGCAGCAGG
  TGCAGGTTGTGGGACTGCAGGAGTACCTGGTCACCTGTGCTGATGATGTCATCAAGATGATCAACATGGG
  CAGTGCCTGCAGGACTTCTGGACAAACATTTGCCAACTCCAATTCCTCCCGCTCCCACGCCTGCTTCCAG
  ATTCTTCTTCGAACTAAAGGGAGATTACATGGCAAATTCTCTTTGGTGGATCTGGCAGGGAATGAACGAG
  GGGCCGACACATCTAGTGCTGACCGGCAGACTCGCATGGAGGGAGCAGAAATTAACAAGTCTCTCCTGGC
  TCTGAAGGAGTGCATCCGGGCGCTGGGACAGAACAAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACC
  CAGGTGCTGAGGGACTCGTTCATTGGGGAGAACTCGAGGACCTGCATGATCGCCATGATCTCACCAGGCA
  TAAGCTCCTGTGAATACACCTTAAACACGCTGAGATACGCAGACAGAGTCAAGGAGCTAAGCCCCCACAG
  CGGACCCAGTGGAGAGCAGCCGGTTCAGATGGAAACAGAAGTGATGGAAGCCAGCTCTAATGGGACGTCT
  CTGACTGGCAATGAAGAGGAGGAGCTGTCTTCCCAGATGTCCAGCTTTAATGAAGCCATGACTCAGATCA
  GGGAGCTGGAAGAGAGGGCCCTGGAAGAGCTCAGAGAGATCATACAGCAAGGACCAAACTGGCTTGAGCT
  CTCCGAGATGACAGATCAGCCAGACTATGACTTGGAGACCTTTGTGAACAAAGCAGAATCTGCCCTGACA
  CAGCAGGCTAAGCAGGCGAAGCACTTCTCAGCCCTTCGAGAAGTCATCAAGGCCCTGCGCCTAGCCATGC
  AACTGGAAGAACAGGCTAGCAAACAAATAAACAGCAAGAAACGGCACCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290662
• Insert Size: 2013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290662.1, NP_001277591.1
• RefSeq Size: 2792 bp
• RefSeq ORF: 2013 bp
• Locus ID: 73804
• Cytogenetics: 4 53.42 cM
• Gene Summary: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:14980225). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.