Acsl4 (NM_019477) Mouse Untagged Clone

CAT#: MC228630

Acsl4 (untagged) - Mouse acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acsl4
• Synonyms: 9430020A05Rik; ACS4; AU018108; Facl4; Lacs4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228630 representing NM_019477
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACT
  TCGACTCACTAGCTGTCATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTAGC
  CAAATTTGGGAAGAAGGACAGCCTTGGAACCCGGGAGATCCTGAGTGAAGAAAATGAAATGCAGCCAAAT
  GGAAAGGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATAAACTATCTTGAAGTGAACTGCAGAG
  TGAATAACTTTGGAAGTGGCCTCACTGCATTGGGACTGAAACCAAAGAACACCATTGCCATTTTCTGTGA
  GACCAGGGCAGAGTGGATGATTGCAGCACAGACTTGCTTTAAGTACAACTTTCCACTTGTGACTTTATAT
  GCCACACTTGGCAGAGAAGCTGTAGTTCATGGATTAAATGAATCTGAGGCTTCCTATCTGATTACTAGTG
  TTGAGCTTCTGGAAAGCAAACTGAAGGCGGCCTTAGTAGATATCAATTGTGTTAAACATATCATTTATGT
  GGATAATAAGACTATCAATAGAGCAGAGTACCCTGAGGGGCTTGAAATTCACAGCATGCAATCAGTAGAG
  GAGCTGGGAGCCAAGCCAGAAAACTTGAGCGTTCCTCCAAGTAGACCAACCCCTTCAGACATGGCCATTG
  TCATGTACACCAGTGGTTCTACGGGCCGCCCCAAGGGAGTGATGATGCATCATAGCAATTTGATTGCTGG
  AATGACAGGCCAGTGTGAACGTATCCCTGGACTAGGACCGAAGGACACATATATTGGCTACTTACCTTTG
  GCTCATGTGCTGGAACTGACAGCAGAGATATCATGCTTTACCTATGGCTGTAGGATTGGATACTCTTCAC
  CCCTTACACTGTCTGACCAGTCCAGCAAAATCAAGAAGGGAAGCAAGGGTGATTGTACTGTACTGAAACC
  CACACTTATGGCCGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAGGTTCAAGAG
  ATGAATTATGTTCAGAAAACTCTATTTAAAATCGGGTATGATTACAAATTAGAGCAAATCAAGAAAGGCT
  ATGACGCCCCTCTTTGTAATCTGATACTGTTTAAAAAGGTGAAGGCTCTGCTGGGAGGGAATGTCCGCAT
  GATGCTGTCTGGCGGCGCGCCACTGTCCCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCC
  ATTGGTCAGGGATATGGGCTGACAGAATCATGTGGTGCTGGAACAGTTACTGAAGTTACTGACTACACTA
  CTGGAAGAGTTGGAGCTCCTCTTATTTGCTGTGAAATTAAACTGAAAGACTGGCAGGAAGGTGGTTATAC
  AGTTCATGATAAGCCGAACCCCAGAGGTGAGATTGTGATCGGTGGCCAGAATATCTCCATGGGATATTTT
  AAAAACGAAGAGAAAACAGCAGAAGATTATTGTGTTGATGAAAATGGACAAAGGTGGTTTTGCACTGGCG
  ATATTGGAGAATTCCATCCTGATGGATGCTTACAGATTATAGATCGTAAGAAAGATCTGGTAAAGTTACA
  AGCAGGAGAATATGTATCTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTGATCGACAACATC
  TGTGCTTTTGCCAAAAGTGACCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAAGTTGACTC
  TTTTGGCACAACAGAAGGGGGTAGAAGGATCTTGGGTTGATATTTGCAATAATCCCGCCATGGAAGCTGA
  AATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCGATCAAGGTT
  CGGTTAAGCCCAGAGCCATGGACCCCCGAGACTGGTTTGGTAACAGATGCCTTCAAGCTGAAAAGGAAGG
  AGTTGAAGAACCATTATCTCAAAGACATTGAGCGAATGTATGGGGGCAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019477
• Insert Size: 2013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019477.3, NP_062350.3
• RefSeq Size: 4990 bp
• RefSeq ORF: 2013 bp
• Locus ID: 50790
• UniProt ID: Q9QUJ7
• Cytogenetics: X F2
• Gene Summary: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.[UniProtKB/Swiss-Prot Function]