Mapk7 (NM_001291035) Mouse Untagged Clone

CAT#: MC228619

Mapk7 (untagged) - Mouse mitogen-activated protein kinase 7 (Mapk7), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk7
• Synonyms: BMK-1; BMK1; ERK-5; ERK5; Erk5-T; PRKM7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228619 representing NM_001291035
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCC
  TGTACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTC
  TAACCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGACTTTGGAATGGCCCGTGGCCTCTGTACT
  TCCCCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCA
  TGCTTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCT
  GGCTCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGA
  ACTCCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCAC
  CAAGGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACG
  CATGTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAG
  TACCATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTA
  CCAGGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCG
  CCAACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATG
  CCCAGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTG
  ATCCTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAGTGAGCTTGCTCCACCAAA
  AAGAGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCCCTGCTCAAGTCCCTAAGGAGC
  AGGCTCAGAGATGGGCCCAGTGCACCCTTGGAGGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAAC
  GCCAGCGAGAACGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACA
  AGAGAGAGAACGCAAGGAGAGGGGGGCTGGTACCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTG
  GTGCTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCC
  CTGCCCCAGCGCCAGCACCAGCGCCAGCACCGTCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGT
  ATCTCAGTCTACTGGTCCTCTACAGCCTGCAGGCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCA
  CCCCCTGGCCCTGTTCCCCAGCCTGCTGGCCCTATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCC
  TTTTGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTA
  CTTCCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGAT
  GTCAACCTGGTGACTCAGCAGCTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCA
  CTCCAAAGGGCAGTGGGGCTGGCTACGGAGTTGGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGA
  TATGGGTGTGGCTGATGGGCCACAGGATGGCCAGGCAGACTCAGCCTCACTCTCAGCCTCTCTCCTTGCT
  GACTGGCTTGAGGGCCATGGCATGAACCCTGCTGACATTGAGTCTCTGCAGCGTGAGATCCAGATGGACT
  CCCCAATGCTGCTGTCTGACCTGCCTGACCTCCAAGAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291035
• Insert Size: 2004 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291035.1, NP_001277964.1
• RefSeq Size: 3265 bp
• RefSeq ORF: 2004 bp
• Locus ID: 23939
• UniProt ID: Q9WVS8
• Cytogenetics: 11 B2
• Gene Summary: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in its 5' UTR uses a downstream start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Both variants 4 and 5 encode the same isoform.