Mid1 (NM_001290505) Mouse Untagged Clone

CAT#: MC228618

Mid1 (untagged) - Mouse midline 1 (Mid1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mid1
• Synonyms: 61B3-R; DXHXS1141; Fxy; Trim18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228618 representing NM_001290505
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC
  CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC
  TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA
  GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATCATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC
  CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCAACACCATGTCCTCTGCCGAGAAGGT
  TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC
  TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
  CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGGTGAATATGTACTG
  TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA
  GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTGGAGAGTAATCTCACCAATCTTATTAAGAGAA
  ACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAAGTCAATGCATCCCG
  TCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAATCATTCAGCAACGAAGACAAATTATT
  GGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTTAGCTCAGCAGATTGCAAACTGTAAAC
  AGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCACTCGCTGAAGGAAAATGACCACGCCCG
  TTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTCCTAATT
  CCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGATTTTTCCCGGGAGAAGAAACTGCTAG
  AATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGAGAAGAGCTCTGCACCGCTTCCTACGA
  CACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA
  TTCACCGGACAAGCCAATGTTGTCAGTCTGTGTAACTCGGCGGACAGCTGGATGATCGTGCCCAACATCA
  AGCAGAACCACTACACCGTGCACGGCCTGCAAAGTGGCACCAAGTATATCTTCACGGTGAAGGCCATCAA
  CCAGGCGGGCAGCCGTAGCAGCGAGCCCGGAAAGCTGAAGACCAACAGTCAGCCGTTTAGACTGGATCCC
  AAATCGGCTCATCGCAAGCTGAAGGTGTCCCACGACAACCTGACTGTCGAGCGCGACGAGTCGTCCTCTA
  AGAAGAGTCACGCGCCGGAGCGCTTCGCTGGTCAGGGGAGCTACGGAGTGGCTGGCAACGTGTTCATCGA
  CAGCGGCCGTCACTACTGGGAAGTGGTCACCAGCGGAAGCACATGGTACGCCATCGGCCTGGCGTACAGA
  TCGGCGCCGAAACACGAGTGGATCGGGAAGAACGCGGCGTCCTGGGCCCTCTGCCGCTGCCACAACCACT
  GGGCGGTGCGACACGACGGCAAGGAGACCCCCATCGCGCCGGCCCCTCACCTCAGGCGCGTCGGCGTCCT
  GCTGGACTACGACAACGGCTCCATCGCCTTCTACGACGCTCTGAGCTCCGTCCACCTCCACACCTTCCAC
  GCGGCGCTCGCGCAGCCCGTGTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACCATCGTCACGGGTC
  TGCCCATCCCGGACCATCTGGACTGTACGGAGCAGCGACCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290505
• Insert Size: 2004 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290505.1, NP_001277434.1
• RefSeq Size: 3938 bp
• RefSeq ORF: 2004 bp
• Locus ID: 17318
• UniProt ID: O70583
• Cytogenetics: X 79.19 cM
• Gene Summary: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 3 and 4 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.