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Rnf6 (NM_001256087) Mouse Untagged Clone

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SKU
MC228616
Rnf6 (untagged) - Mouse ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 3
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $673.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Rnf6
Synonyms 1200013I08Rik; 5730419H05Rik; AA537053
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC228616 representing NM_001256087
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCGTCTAGATCTAGATCAGGTGGGAGTGGAGAAGAGAGCTCATTTCAAGAAAATGAGAGAAGAT
GGCAGCAAGAACGTCTCCACAGAGAAGAAGCCTATTATCAGTTTATTAATGAGCTCAGTGATGAAGATTA
TCGGCTAATGAGAGACCATAATCTTTTAGGCACCCCTGGAGAAATAACATCAGAGGAGCTGCAGCAGCGC
TTAGAGAGAGCAAAGGAACAGCTGGCATCTCAGCCTGGCTCTGACAGCGCGGCAAGTGATGGAGACTCAG
AAAGCCTTAGAGCACATTCAGATGAGGACTCTCTGCTGCGATGGCTGAATACCTTTCGGCGCACTGGGAA
TGTGACTCGAAGCGGACAGAATGGGAACCAAAGCTGGAGGGCCGTGAGTCGAACAAACCCGAACAGTGGC
GAGTTCGGGTTTAGCCTGGAAATCCACATAAACCCTGACAACAGAGGTTCTGAAATGCATGGAGAGGACT
CGACGGACATCCCTCTGTCAGGTGTTAACAGAGAGCATAGGCAGCAAAGACCATCGAGTCCTGTGGCTAG
GCGAACCAGAAGCCAGACCTCCATGAGCTCCAGTGGTCCTAGGGGCAGGCGTGGTGCGAGGAGGCAGGGT
TCTGTGCAAGGGTCATTTGCAACTCTGGGAAGGTTAAGGAATGGAATTGGAGTAGCCCTTGGTGTTCCCA
GAGTTAGTGCCCCTCGCACTAATGTTATTAATTCCCACACCAACCAGTCAGATGGTAGTACTCTCAGGCA
AGGTGGACGGCAGCGGTTTGGAGCAGCACATATTTGGGAAAACGGGGCTAGAAGTAATGTTACAGTGAGG
AACACAAACCAAAGATTAGAGCCAATAAGATTACGTCCTGCTTTCAGTAGTCGAAGCCGGTCCCCCATTC
AGAGACAGAATGGTACTGTTCATCATAATTCCCAAAGGCAGGGTAGACCAGTACAGCAAACGGGTAGAAA
CAGGTCTGTTAGGCACAGAGGTGTAACGCGGGTCTTTTTAGAACAAGGTAGAGAACACAGAGGCACTGAT
TACACCCCGCTGTCCAACTCAAGACTTGTGTCAAGAATAACAGTAGAAGAGGGAGAGTCTAGCAGGTCCT
CAGCTGCCACCCAGCGTCATCCAGCAATCACACTGGACCTGCAAGTCAGGAGGATTCGGCCTGGAGAAAC
TAGAGATAGGGATAGCATTGCAAATAGAACCCGATCTAGGGCGGGGCTGGCAGAAAGTACGGTTGAGAGC
ACCAGTGGGGGCTTCCACAGAACTATCTCTCACTTAGAGCGCTCAGGTGTCCGAACCTACGTGAGCACCA
TCACAGTTCCTCTTCGCAGGATTTCTGAGAATGACGTGGTCGAGCCATCCTCCGTGGCTCTTCGGTCAAT
TTTAAGGCAGATCATGACTGGGTTTGGAGAGCTGAGTTCTCTAATGGAGGTGGAGCCTGCGTCAGAGAAT
CAAAGCAATGGCCAACGTTTACCAGAGGTGTACCTAGAACTGAGTAATGGAGATGCAGCCGATGACAGTG
GTCAGCATGGAAGAGCGTCCTCACAGGCCAGTCAGGCCCAGGACGGTGCTGAAATGCTAGCTGTGAGGGA
GCCCGCCCCGCCCCAGGCTCGCCCCAGTGGCAGTAGAAGTAGAAGGCAGTTAGGAAGGGCGGACAGCGTA
GTTGAGGCTGGAACATTACCCATTCTGCGCCTTGCTCACTTCTTCTTACTGAATGAAGGCGATGATGATC
CAATTCGTGGTTTAACCAAAGAGCAGATTGACAATCTTTCTACCAGGAGTTATGAACAAGACGGGGTTGA
CAGTGAGTTAGGTAAGGTGTGTAGCGTGTGCATCAGTGACTACGTGGCTGGGAACAAGCTCCGGCAGCTG
CCTTGTCTGCACGAGTTTCACATTCACTGTATTGATCGCTGGCTGTCGGAGAACTGCACCTGCCCCGTCT
GTCGGCGGCCTGTCTTAGAATTCGGAGCCACAAGCAGTGGGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001256087
Insert Size 2004 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001256087.1, NP_001243016.1
RefSeq Size 3286 bp
RefSeq ORF 2004 bp
Locus ID 74132
UniProt ID Q9DBU5
Cytogenetics 5 G3
Summary E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same isoform (1).
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Documents
Resources
"NM_001256087" in other vectors (1)
SKU Description Size Price
MR230827 Rnf6 (myc-DDK-tagged) - Mouse ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 3 10 ug
$589.00 $611.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.