Picalm (NM_001252520) Mouse Untagged Clone

CAT#: MC228589

Picalm (untagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Picalm
• Synonyms: CALM; CLTH; fit-1; fit1; mKIAA4114; PAP180
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228589 representing NM_001252520
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGCCAGAGCCTGACGGACCGAATCACCGCGGCCCAGCACAGTGTCACTGGCTCCGCGGTATCTA
  AGACAGTATGCAAGGCCACGACCCACGAGATCATGGGCCCCAAGAAAAAGCACCTGGACTACTTAATTCA
  GTGTACAAATGAGATGAATGTGAACATCCCACAGTTGGCGGACAGTTTGTTTGAAAGAACTACTAACAGT
  AGTTGGGTGGTGGTCTTCAAATCACTCATTACAACTCATCATTTGATGGTGTATGGAAATGAGCGTTTCA
  TTCAGTATTTGGCTTCAAGAAACACATTGTTTAACTTAAGCAATTTTTTGGATAAAAGTGGATTGCAAGG
  ATATGATATGTCTACATTTATTAGACGGTATAGTAGATACTTAAATGAAAAAGCAGTTTCATACAGACAA
  GTTGCATTCGATTTCACAAAAGTGAAGAGAGGAGCTGATGGAGTTATGAGAACAATGAACACAGAAAAAC
  TCTTAAAAACTGTACCAATTATCCAAAATCAAATGGATGCACTTCTTGATTTTAATGTTAATAGTAATGA
  ACTTACAAATGGGGTAATAAATGCTGCCTTCATGCTCCTCTTCAAAGATGCCATTAGACTATTTGCAGCA
  TACAATGAAGGAATTATTAATTTATTGGAAAAATATTTTGATATGAAAAAGAACCAGTGCAAAGAAGGTC
  TTGACATCTATAAGAAGTTTCTGACTAGGATGACAAGAATCTCAGAGTTTCTGAAAGTTGCAGAGCAAGT
  TGGAATTGACAGAGGAGATATTCCAGATCTTTCACAGGCCCCCAGCAGTCTTCTTGATGCTTTAGAACAA
  CATTTAGCTTCCTTGGAAGGAAAGAAAATCAAAGATTCCACAGCTGCAAGCAGGGCTACAACACTTTCCA
  ATGCAGTCTCTTCTTTGGCAAGCACTGGCCTATCTCTGACCAAAGTGGATGAAAGGGAAAAACAGGCAGC
  ATTAGAGGAAGAACAGGCTCGTTTAAAAGCATTAAAGGAACAGCGTCTAAAAGAACTTGCAAAGAAACCG
  CATACCTCTCTAACAACTGCAGCCTCTCCGGTGTCCACCTCAGCAGGGGGAATAATGACTGCACCAGCCA
  TTGACATATTTTCTACCCCTAGTTCTTCTAACAGCACATCTAAGCTGCCAAATGACCTGCTTGACTTGCA
  GCAGCCAACCTTTCACCCGTCTGTCCACGCCATGTCGGCTGCTCCTCAGGGAGCGAGTACATGGGGAGAT
  CCTTTCTCTGCTACTCTAGATGCTGTTGAGGATGCCATTCCAAGCTTAAACCCTTTCCTCACAAAAAGTA
  GTGGTGATGTTCACCTGCCCATTGCTTCAGATGTATCCACTTTTACTACTAGGACACCTACTCATGAGAT
  GTTTGTTGGATTCAGTCCTTCTCCAGTTGCACAGCCACATTCTTCAGCTGGCCTTAATGTTGACTTTGAA
  TCTGTGTTTGGAAATAAATCTACGAATGTTGCTGTAGATTCTGGTGGTGGACTTCTCAAACCAACAGTGG
  CCTCCCAGAACCAGAGTCTTCCTGTTGCCAAACTTCCGCCTAACAAATTAGTGTCTGACGACTTGGATTC
  ATCTTTAGCCAACCTTGTGGGCAATCTTGGCATTGGAAATGGAACCACTAAGAATGATGTAAGTTGGAGT
  CAACCAGGTGAAAAGAAGTTAACTGGAGGATCTAACTGGCAACCAAAGGTTGCACCAACAACTGCCTGGA
  GCGCTGCAACAATGAATGGCATGCATTTTCCACAATACGCGCCCCCTGTAATGGCCTATCCTGCTACTAC
  ACCAACGGGCATGATAGGATATGGAATTCCTCCTCAGATGGGAAGTGTACCTGTAATGACACAACCAACC
  TTAATATACAGCCAGCCTGTCATGAGACCACCAAACCCCTTTGGCCCTGTATCAGGAGCACAGATACAGT
  TTATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252520
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252520.1, NP_001239449.1
• RefSeq Size: 4159 bp
• RefSeq ORF: 1968 bp
• Locus ID: 233489
• UniProt ID: Q7M6Y3
• Cytogenetics: 7 50.47 cM
• Gene Summary: Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).[UniProtKB/Swiss-Prot Function]