Ablim1 (NM_001290815) Mouse Untagged Clone

CAT#: MC228587

Ablim1 (untagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ablim1
• Synonyms: 2210411C18Rik; 2610209L21Rik; 4833406P10Rik; 9330196J19Rik; AV079770; AW060987
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228587 representing NM_001290815
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAAGAAAAAGTGGCCCATCCTCAGGACCCTCACCACTCGTCGGAGAAACCTGTCATTCACTGCC
  ATAAATGCGGGGAGCCATGCAAGGGCGAAGTGCTACGGGTGCAGACCAAGCATTTCCACATCAAATGCTT
  CACCTGCAAAGTGTGCGGCTGCGACCTGGCCCAGGGGGGCTTCTTCATAAAGAACGGGGACTATCTGTGT
  ACCCTGGACTACCAGAGGATGTATGGGACACGGTGCCACGGCTGTGGGGAGTTCGTGGAGGGCGAGGTGG
  TAACTGCCCTGGGCAAGACCTACCACCCCAACTGCTTCGCCTGTACAATCTGCAAGCGCCCATTTCCACC
  CGGAGACCGAGTCACATTCAACGGGAGAGACTGTCTTTGTCAGCTCTGTGCACAGCCGATGTCTTCCAGC
  CCTAAAGAAGCCTCCTGCTCCAGCAACTGCGCCGGCTGTGGAAGGGACATTAAGAACGGACAGGCGCTGC
  TAGCTCTGGACAAGCAGTGGCATTTGGGCTGCTTCAAGTGCAAGTCCTGTGGGAAGGTCCTCACCGGCGA
  GTACATCAGCAAGGATGGCTCCCCATACTGTGAGAAAGACTACCAGGGGCTCTTTGGGGTGAAGTGTGAA
  GCCTGTCACCAGTTTATCACAGGAAAAGTCCTGGAGGCAGGCGACAAGCATTATCATCCCAGCTGCGCAC
  GATGCAGCAGATGCAATCAGATGTTCACAGAAGGGGAAGAAATGTATCTGCAAGGTTCTACCGTGTGGCA
  TCCCGACTGTAAGCAGTCCACTAAGACAGAGGAGAAGCTGCGGCCCACCAGGACCTCCTCTGAAAGTATC
  TATTCTAGACCAGGCTCCAGCATCCCTGGTTCACCAGGCCATACTATCTATGCAAAAGTAGACAATGAAA
  TCCTGGATTACAAGGATTTAGCAGCCATCCCCAAGGTCAAGGCGATCTATGACATCGAGCGTCCAGATCT
  CATTACCTATGAGCCTTTCTACACATCAGGCTATGAGGACAAGCAGGAGAGACAGAGCCTTGGAGAGTCT
  CCAAGGACTTTGTCTCCAACTCCATCTGCAGAAGGTTATCAAGATGTCCGGGATCGGATGATCCACAGGT
  CCACCAGCCAGGGCTCCATCAACTCCCCTGTGTATAGCCGGCACAGTTACACTCCAACTACGTCTCGCTC
  GCCCCAGCACTTCCACCGACCTGGCAATGAGCCGTCCAGCGGCCGGAACTCCCCTCTCCCCTACCGGCCC
  GACAGCCGCCCTCTAACTCCAACTTACGCTCAGGCCCCTAAACATTTCCATGTTCCAGATCAAGGGATCA
  ACATTTACCGAAAACCACCCATCTACAAACAGCATGGAACTGACCCGAGGCGCAGATCAAGTGGCAGAGA
  GGAAGATGAAGAGGAGCTTTTGAGACGCCGGCAGCTTCAAGAAGAACAGTTGATGAAGCTCAACTCAGGC
  CTGGGGCAGCTGATCCTGAAGGAAGAGATGGAGAAGGAGAGCAGAGAAAGAGCATCGCTGGCCAGTCGCT
  ATGACTCTCCCCTCCACTCAGCTTCCCATGCTCCATCATCTAAAACTTCATCTCTCCCTGGTTATGGAAA
  GAATGGCCTTCACCGGCCAGTTTCCACAGACTTCGCTCAGTACAACAGCTACGGAGACATCAGTGGCGGA
  GTTCGAGACTACCAGACCCTCCCAGATGGCCACATGCCTGCAGTGAGAATGGACAGAGGAGTATCCATGC
  CCAACATGTTGGAACCAAAGATATTTCCATATGAGATGCTCATGGTGACCAACAGAGGGCGCAACAAAAT
  CCTGAGAGATGTGGATAGAACCCGGCTAGAGCGCCACTTAGCCCCAGAAGTATTTTGGGAGATCTTTGGG
  ATGTCCATACAGGAATTTGACAAGTTACCTCTTTGGAGACGCAACGACATGAAGAAAAAAGCTAAACTCT
  TCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290815
• Insert Size: 1965 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290815.1, NP_001277744.1
• RefSeq Size: 6259 bp
• RefSeq ORF: 1965 bp
• Locus ID: 226251
• UniProt ID: Q8K4G5
• Cytogenetics: 19 52.09 cM
• Gene Summary: May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and lacks four alternate in-frame exons in the 3' coding region, compared to variant 1. It initiates translation at an alternate start codon. The encoded isoform (5) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.