Tcf25 (NM_001286363) Mouse Untagged Clone

CAT#: MC228580

Tcf25 (untagged) - Mouse transcription factor 25 (basic helix-loop-helix) (Tcf25), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcf25
• Synonyms: 1100001J13Rik; 1810041K11Rik; D8Ertd325e; mKIAA1049; Nulp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228580 representing NM_001286363
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGCCGGGCCCTCCGGAGACTGAGGGGGGAACAGCGCGGCCAGGAACCCCTAGGGCCCGACGCCT
  TGAAGTTTGTCCTCCTTGATGACGATGACGCAGAGGAAGAGGGACCAAAACCGGGGCTGGGTGGCCGGCG
  CCCCGGGGGTGCAGGGAAGGAAGGAGTCCGTGTCAACAACCGGTTTGAGCTGATAAACACGGAGGATCTT
  GAGGATGACCTTGTGGTAAATGGGGAGAGATCTGACTGTACCCTGCCGGACTCTGTGTCATCAGGGAACA
  AAGGAAGGGCTAAACATGGAAACGCAGAGACCAAGCAAGATGGAGGGGCCACCAAAGCAGGGTCCTCGGA
  ACAGGAGAATGGACTAGAAGACATCGATCGCATCTTAGAGCGGATTGAGGACAGCAGTGGGTTCAGCCAC
  CCCGGCCCCCCTCCACTGAGCTCCAGGAAGCACGTCCTATATGTGGAGCACAGACACCTGAATCCAGACA
  CGGAACTGAAGAGGTATTTTGGTGCTCGAGCGGTCCTGGGCGAGCAAAGGCCTCGACAGAGGCAGCGTGT
  GTATCCCAAGTGTACATGGCTGACGACCCCTAAGAGCACCTGGCCCCGGTACAGCAAACCAGGTCTATCA
  ATGCGGCTGCTGGAGTCCAAGAAAGGCCTTTCTTTCTTTGCATTTGACCATAATGAAGAATACCAACAAG
  CACAGCACAAGTTCCTGGTGGCTGTGGAGTCCATGGAGCCAAATAACATTGTGGTTCTGCTCCAGACAAG
  CCCCTATCATGTGGACTCCCTGCTGCAGCTCAGTGATGCCTGCCGCTTTCAAGAGGATCAAGAGATGGCT
  CGAGACCTTATAGAGAGAGCCCTGTACAGCATGGAGTGTGCATTCCACCCCTTGTTCAGTCTCACCAGTG
  GAACTTGCAGGCTGGATTATCGCAGACCTGAGAATAGGAGCTTCTACCTGACCCTCTACAAGCAGATGAG
  TTTCTTGGAGAAACGTGGATGCCCACGTACTGCGCTGGAGTACTGCAAGCTCATCCTGAGCCTTGAGCCA
  GATGAGGACCCCCTGTGCATGTTGCTGCTTATTGACCACCTGGCCTTACGGGCCCGGAACTATGAATACC
  TGATTCGCCTCTTCCAGGAATGGGAGGCTCATCGGAACCTGTCCCAGCTCCCAAATTTTGCCTTCTCAGT
  TCCACTGGCCTATTTCCTTCTGAGTCAGCAAACAGACTTACCTGAGCATGAGCTCAGCTCTGCCAGGCAA
  CAGGCTTCCCTCCTGATACAGCAGGCACTCACCATGTTCCCTGGAGTTCTTATGCCACTGCTGGAGTATT
  GCAGTGTGCGACCTGATGCCACGGTCTCAAACCACCGCTTCTTTGGGCCGGATGCTGAGATCAGCCAGCC
  TCCCGCCTTGGGCCAGCTGGTGAGTCTGTACCTGGGGAGGTCACACTTTCTTTGGAAAGAACCCGCCATT
  ATGAGCTGGCTAGAGGAAAATGTCCATGAGGTTCTGCAAGCGGTGGATGCTGGAGACCCTGCTGTGGAGG
  CCTGTGAGAACAGGCGGAAAGTGTTGTACCAGCGTGCGCCCAGAAACATCCACCGCCACGTGATACTGTC
  TGAGATCAAGGAGGCTGTTGCTGCCCTACCCTCGGATGTGACCACACAGTCTGTGATGGGGTTCGACCCT
  CTGCCTCCCCTGGACACCATCTACTCTTACGTCAGACCAGAGAGGCTGAGTCCAGTCAGCCACGGAAACA
  CAATCGCCCTCTTCTTCCGGTCCTTGTTGCCAAATTACACCACAGAGGGGGAGAGGCTGGAGGAAGGAGT
  GGCCGGGGGTCCGAACCGCAATCAAGGCTTGAACAGGCTGATGTTGGCCGTACGTGACATGATGGCCAAC
  TTCCACTTCAACGACCTGGAGGTGCCTCGGGAGGACAATCCTGAGGGCGAGGGGGACTGGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286363
• Insert Size: 1956 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286363.1, NP_001273292.1
• RefSeq Size: 2776 bp
• RefSeq ORF: 1956 bp
• Locus ID: 66855
• Cytogenetics: 8 72.1 cM
• Gene Summary: May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development (By similarity).[UniProtKB/Swiss-Prot Function]