Ralbp1 (NM_001198949) Mouse Untagged Clone

CAT#: MC228575

Ralbp1 (untagged) - Mouse ralA binding protein 1 (Ralbp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ralbp1
• Synonyms: Rip1; RLIP76
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228575 representing NM_001198949
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAGTGCTTCCTGCCCCCGAGCAGCAGCCCTAGTGAACACCGCAGGGCCGAGCATGGCAGCGGGC
  TGACCCGGACCCCCAGCTCTGAGGAGATCAGTCCTACAAAGTTCCCTGGATTGTACCGGACAGGCGAGCC
  CTCGCCTCCCCATGACGTCCTCCATGAGCCTCCTGATACAGTGTCAGATGACGACAAAGACCATGGAAAG
  AAAAAAGGGAAATTTAAGAAAAAGGAGAAAAGGACTGAAGGCTACGCTGCCTTCCAGGAAGACAGCTCTG
  GAGATGAAGCTGAAAGTCCTTCCAAGGTGAAGAGGTCCAAGGGAATCCATGTGTTCAAAAAGCCCAGTTT
  CTCTAAAAAGAAGGAAAAAGATTTTAAAATCAAAGAAAAACCCAAAGAAGAAAAGCATAAAGAAGAAAAG
  CACAAAGAGGAAAAGCATAAAGAAAAGAAATCGAAAGACTTGACAGCAGCTGATGTTGTTAAACAGTGGA
  AGGAAAAGAAGAAAAAGAAAAAGCCAATTCAGGAGCCAGAGGTGCCTCAGATGGACGCACCGAGTGTCAA
  GCCCATTTTTGGGGTTCCCTTGGTTGACGCAGTAGAGAGGACCATGATGTACGACGGCGTCCGGCTGCCA
  GCTGTCTTCCGGGAGTGTGTGGACTACATGGAGAAGCACGGCATGAAGTGTGAAGGCGTCTACCGGGTGT
  CAGGAATCAAATCAAAGGTAGATGAGCTAAAAGCAGCCTATGACCGAGAGGAGTCTCCAAACTTAGAAGA
  ATATGAGCCTAACACTGTAGCCAGCTTGCTGAAGCAGTATTTGCGAGACCTTCCAGAGAATTTGCTTACC
  AAAGAGCTTATGCCCCGGTTTGAAGAGGCTTGTGGGAAGACCACAGAGATGGAGAAAGTGCAGGAATTCC
  AGCGCTTGCTCCGGGAACTGCCGGAGTGCAATCATCTTCTGCTTTCCTGGCTCATTGTGCACCTGGACCA
  CGTCATTGCGAAGGAGCTGGAAACGAAGATGAACATCCAGAACATCTCTATAGTGCTGAGCCCCACCGTG
  CAGATCAGCAATCGGGTCCTGTACGTGCTTTTCACACATGTGCAAGAGCTCTTTGGCACCGTGGTCCTGA
  AGCAAGTAACAAGACCTCTGCGCTGGTCCAACATGGCCACGATGCCCACACTGCCAGAGACCCAAGCAGG
  CATCAAGGAGGAGATCAGGAGACAGGAGTTCCTTTTGAATTGTTTACATCGAGATCTGCAGGGCGGGATA
  AAGGACTTATCTAAAGAAGAAAGATTATGGGAAGTACAGAGGATTCTGACTGCCCTCAAGAGAAAGCTGA
  GGGAAGCTAAAAGACAAGAGTGTGAAACCAAGATTGCACAGGAGATAGCTAGTCTTTCAAAAGAGGATGT
  TTCCAAAGAAGAAATGAATGAAAACGAAGAAGTTATAAATATCCTTCTTGCCCAGGAGAATGAAATCCTG
  ACAGAACAGGAGGAGCTCCTGGCCATGGAGCAGTTCCTGCGTCGTCAGATCGCCTCAGAGAAAGAAGAGA
  TCGACCGCCTCCGAGCTGAAATTGCGGAGATCCAGAGCCGTCAGCAGCATGGCCGCAGTGAGACGGAGGA
  GTACTCGTCCGACAGCGAGAGTGAGAGCGAGGACGAGGAGGAGCTGCAGCTCATCCTGGAGGACCTGCAG
  CGGCAGAACGAGGAGTTAGAAATAAAGAACAATCACTTGAACCAAGCAGTCCATGAGGAGCGGGAGGCCA
  TCATCGAGCTCCGAGTGCAGCTCCGGCTGCTCCAGATGCAGCGAGCCAAGTCTGAGCAGCAGCCGCAGGA
  GGAGGAGGAGCCCGAGAGGCGAGGGGGCATAGGCCCACCGCCCTGTGACGGTGTCCTCGAGGTGCGGGTA
  GCCAAGGAGCAGGCGAAGGCCTCGCCCAGCAAAGACCGGAAGGAGACGCCCATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198949
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198949.1, NP_001185878.1
• RefSeq Size: 3788 bp
• RefSeq ORF: 1947 bp
• Locus ID: 19765
• UniProt ID: Q62172
• Cytogenetics: 17 35.26 cM
• Gene Summary: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.