Rhbdf1 (NM_001291818) Mouse Untagged Clone

CAT#: MC228562

Rhbdf1 (untagged) - Mouse rhomboid family 1 (Drosophila) (Rhbdf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rhbdf1
• Synonyms: C16ORF8; Dist; Dist1; Egfr-rs; mKIAA4242
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228562 representing NM_001291818
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCGGGCCGCTGCAGCATTGGTGAAGGGTCGGTCTATTAGGGATGGCACTTTACGAAGGGGAC
  AGCGCCGAAGCTTTACCCCTGCCAGCTTTCTTGAGGAAGACATGGTGGACTTCCCTGATGAGTTGGATAC
  ATCTTTCTTTGCTCGGGAAGGTGTCCTCCACGAGGAGATGTCCACGTACCCAGATGAGGTGTTTGAGTCC
  CCATCAGAGGCAGCGCTCAAGGACTGGGAGAAAGCCCCGGATCAGGCCGACCTCACGGGTGGGGCCCTGG
  ACCGCAGTGAGCTTGAGAGAAGCCACTTGATGTTGCCCCTGGAACGAGGCTGGAGAAAGCAGAAGGAAGG
  CGGCCCTCTAGCCCCGCAGCCCAAGGTGCGGCTTCGCCAGGAGGTGGTCAGTGCGGCGGGACCCAGGAGG
  GGTCAGCGTATCGCTGTGCCAGTGCGCAAACTCTTCGCTCGGGAAAAGCGGCCTTATGGTCTGGGGATGG
  TGGGACGGCTCACCAACCGCACGTACCGGAAACGCATCGACAGCTATGTCAAGCGGCAGATCGAGGACAT
  GGATGATCACAGGCCCTTCTTCACTTACTGGCTCACCTTTGTGCACTCACTGGTCACCATTCTGGCTGTG
  TGCATCTATGGCATAGCGCCCGTGGGCTTCTCGCAGCATGAGACGGTGGACTCGGTACTTCGGAAGCGGG
  GTGTCTATGAGAATGTCAAATACGTCCAGCAGGAGAACTTCTGGATTGGCCCCAGCTCGGAGGCCCTCAT
  TCACTTGGGTGCCAAGTTCTCACCCTGCATGCGTCAGGACCCACAGGTGCACAGCTTCATCCTGGCTGCC
  CGGGAGCGTGAAAAGCACTCGGCTTGCTGTGTACGCAATGATCGGTCTGGGTGTGTGCAGACTTCCAAGG
  AGGAGTGCTCGTCCACGTTGGCGGTGTGGGTGAAGTGGCCCGTTCATCCTAGTGCCCCAGACCTTGCAGG
  CAACAAGAGACAGTTTGGTTCTGTCTGCCACCAGGACCCCAGGGTATGTGACGAGCCTTCCTCTGAGGAT
  CCCCACGAGTGGCCAGAAGATATCACCAAGTGGCCGATCTGCACCAAAAGCAGCGCGGGGAACCACACTA
  ACCACCCTCACATGGATTGTGTCATAACAGGCCGGCCTTGCTGCATCGGCACCAAGGGCAGGTGTGAGAT
  CACCTCCCGGGAGTACTGTGACTTCATGAGGGGCTACTTCCATGAGGAGGCCACACTCTGCTCTCAGGTG
  CACTGCATGGACGATGTCTGTGGCCTCCTGCCTTTCCTCAATCCTGAGGTGCCTGACCAGTTCTACCGCC
  TGTGGCTGTCCCTCTTCCTGCACGCTGGGATCCTGCACTGCTTGGTGTCCGTCTGCTTCCAGATGACGGT
  CCTGCGGGACCTGGAGAAGCTGGCAGGCTGGCACCGCATAGCCATCATCTACCTGCTGAGTGGTATCACG
  GGCAATCTAGCCAGTGCCATCTTCCTGCCATACCGGGCAGAGGTAGGTCCGGCCGGCTCCCAGTTTGGCA
  TCCTGGCCTGCCTCTTTGTGGAGTTGTTCCAGAGCTGGCAGATCCTGGCCCGACCCTGGCGTGCCTTCTT
  CAAGCTCCTGGCCGTGGTGCTCTTCCTCTTTGCCTTTGGGCTGCTGCCCTGGATCGACAACTTCGCCCAC
  ATCTCGGGCTTTGTCAGCGGCCTCTTCCTTTCCTTTGCCTTCCTGCCTTACATCAGCTTTGGCAAGTTCG
  ACCTGTACCGCAAGCGCTGCCAGATCATCATCTTCCAGGTCGTCTTCCTGGGCCTGCTGGCCGGCCTGGT
  GGTCCTCTTCTACTTCTACCCGGTGCGCTGTGAATGGTGCGAGTTCCTCACCTGCATCCCTTTCACGGAC
  AAGTTCTGTGAAAAGTACGAGCTGGACGCTCAGCTCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291818
• Insert Size: 1932 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291818.1, NP_001278747.1
• RefSeq Size: 2836 bp
• RefSeq ORF: 1932 bp
• Locus ID: 13650
• Cytogenetics: 11 18.83 cM
• Gene Summary: Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) use an alternate splice junction at the 5' end of an exon and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.