Abr (NM_001291186) Mouse Untagged Clone

CAT#: MC228555

Abr (untagged) - Mouse active BCR-related gene (Abr), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228555 representing NM_001291186
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCCTGCTCATCATTCGCTTCTGCTGTAACTGCACCTATGCTCTGCTGTACAAGCCAATTGACC
  GAGTCACCCGGAGCACCCTGGTCCTTCACGATCTGTTGAAGCACACACCCGTGGACCACCCGGACTACCC
  GCTGCTTCAGGATGCTCTCCGCATCTCCCAGAATTTCCTGTCTAGCATTAACGAGGACATTGATCCTCGC
  CGGACTGCAGTCACAACCCCCAAGGGAGAGACTCGGCAGCTGGTGAAGGACGGCTTCCTGGTGGAAATGT
  CTGAGAGCTCCCGGAAGCTGCGGCATGTCTTTCTCTTCACAGATGTTCTGCTGTGTGCCAAGCTAAAGAA
  GACCTCAGCAGGAAAGCATCAGCAGTATGACTGTAAGTGGTACATCCCTCTGGCTGACTTAGTGTTCCCA
  TCCCCTGAGGAGTCAGAGGCCAGTCCCCAGGTGCACCCCTTCCCAGACCACGAGCTCGAGGACATGAAAA
  CGAAGATCTCTGCCCTCAAGAGTGAGATCCAGAAGGAGAAAGCCAACAAAGGACAGAGCCGAGCCATTGA
  GCGCCTCAAAAAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATCCCTTTCCGG
  ATACACAATCGGAATGGAAAGAGTTACCTGTTTCTTCTGTCTTCTGACTATGAGAGGTCGGAGTGGAGAG
  AAGCGATTCAGAAGCTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCTGTGGAGCTCCAGGTGCT
  CACGGGATCCTGTTTCAAGCTTAGGACTGTGCACAACATTCCTGTCACCAGCAATAAAGATGATGATGAG
  TCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCCAATC
  TTTACTGTACTCTGGAGGTGGATTCCTTTGGCTATTTTGTCAGCAAAGCCAAGACCAGGGTGTTTCGGGA
  CACGACAGAGCCCAAGTGGGATGAGGAGTTTGAGATTGAGCTGGAGGGTTCACAGTCCCTGAGGATCCTG
  TGTTATGAGAAGTGCTACGACAAGACCAAGGTCAACAAAGACAACAATGAGATTGTGGACAAGATCATGG
  GCAAGGGGCAGATCCAGCTGGATCCACAGACAGTAGAAAGCAAGAACTGGCATACGGACGTGATTGAAAT
  GAATGGGATCAAAGTGGAATTCTCCATGAAATTTACCAGCCGTGACATGAGCCTGAAGAGGACCCCATCC
  AAAAAGCAGACAGGCGTCTTTGGAGTGAAGATCAGCGTGGTGACCAAGCGGGAGCGCTCCAAGGTGCCCT
  ACATCGTCCGGCAATGCATAGAAGAGGTGGAGAAGAGGGGCATTGAGGAGGTTGGCATCTACAGGATATC
  AGGGGTGGCCACGGACATCCAGGCCCTGAAGGCTGTCTTTGATGCCAATAACAAGGACATCCTACTGATG
  CTGAGCGACATGGACATCAACGCCATCGCTGGGACCCTCAAGCTATACTTTCGGGAGCTGCCTGAGCCCC
  TCCTTACAGATAGACTTTATCCAGCCTTCATGGAGGGCATTGCCCTGTCAGACCCTGCTGCCAAGGAGAA
  CTGCATGATGCACCTACTCCGCTCCCTGCCTGACCCCAACCTCATCACCTTCCTTTTCCTGCTGGAACAC
  TTGAAAAGGGTTGCTGAGAAGGAGCCCATCAACAAGATGTCACTTCACAACCTTGCCACGGTGTTTGGCC
  CCACGTTACTGAGACCATCAGAAGTGGAGAGCAAAGCACATCTCACATCAGCTGCAGACATCTGGTCCCA
  TGATGTCATGGCCCAGGTCCAGGTCCTCCTCTACTACCTGCAGCATCCCCCCATTTCCTTCGCAGAACTG
  AAGCGGAACACACTGTACTTCTCCACAGACGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291186
• Insert Size: 1926 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291186.1, NP_001278115.1
• RefSeq Size: 4463 bp
• RefSeq ORF: 1926 bp
• Locus ID: 109934
• UniProt ID: Q5SSL4
• Cytogenetics: 11 45.92 cM
• Gene Summary: Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (By similarity). Functions as an important negative regulator of neuronal RAC1 activity (PubMed:20962234). Regulates macrophage functions such as CSF-1 directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks part of the 5' coding region, contains an alternate 5'-terminal exon, and uses an alternate start codon, compared to variant 3. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 3.