Gga3 (NM_001252067) Mouse Untagged Clone

CAT#: MC228553

Gga3 (untagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gga3
• Synonyms: C230037M19Rik; mKIAA0154
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228553 representing NM_001252067
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGGAAGGGGAGAGCCTGGAGTCCTGGCTGAATAAAGCTACCAATCCTTCCAACCGCCAGG
  AAGACTGGGAATATATCATTGGTTTCTGTGATCAGATCAACAAAGAGCTTGAAGGGCCCCAGATTGCTGT
  GCGACTCCTGGCCCACAAGATCCAGTCTCCACAGGAATGGGAGGCAGTGCAGGCCCTCACGGTGCTGGAG
  GCGTGTATGAAGAACTGTGGCAGGAGACTTCATAATGAAGTGGGGAAGTTCCGGTTTTTGAATGAATTAA
  TCAAAGTCGTCTCTCCAAAGTACTTGGGAGACAGGGTGTCTGAAAAGGTGAAGACTAAGGTCATCGAGTT
  GCTTTTTAGCTGGACACTGGCCTTGCCGGAAGAAGCAAAGATCAAAGATGCCTACCATATGTTGAAGAGA
  CAAGGCATAGTGCAGTCGGACCCCCCCATCCCCATGGATAGAACGCTGATCCCCTCTCCCCCACCTCGGC
  CCAAAAACCCCGTTTTTGATGATGAGGAGAAATCCAAGCTTTTAGCCAGGCTGCTGAAGAGCAAGAACCC
  AGATGACCTGCAAGAGGCCAACAGACTCATCAAGTCCATGGTGAAGGAAGATGAGGCTCGCATCCAGAAA
  GTGACCAAGCGTCTACACACACTGGAGGAGGTTAACAACAATGTCAAGCTGCTCCACGAGATGCTGCTTC
  ACTACAGCCAAGAGTACTCGTCAGACGCCGACAAGGAGCTCATGAAGGAGCTCTTTGATCGTTGTGAGAA
  TAAGAGGCGGACGCTGTTTAAACTAGCCAGCGAGACAGAGGACAATGACAACAGTTTGGGGGACATCCTG
  CAGGCCAGTGACAACCTGTCCAGGGTCATCAACTCTTACAAAACTATTATTGAAGGGCAGATCGTCAATG
  GTGAGGTGACCACCTCAACCATGCCTGACTCTGAAGGCCTTACTGACCCCGCCCCCACTGCTCCCAAAGA
  GTCACCTGGAAGTAGTCAGTGGCACCTGTTCCAGAATGAACCACCATCAGATCTGGACTTCTTCAGTCCC
  AGGCCAGTGCCTGCGGCCTCCTGCCCCTCAGATGGACCCCAGCTCCCTCCCCCAGTTTCTACCTCAAGTA
  TGTCCCAAGCTCCACTGCCTGCTGCCTTCCCAGCTCCTGTGGTCCCAGCCAGTGCACCAACCCACAGCAC
  TGGCTCCTTCATGTTCTCTTCTGGACCTGCCCCAGCCTTGGCTCCAAAGGCTGAGCCCAAAGGTCCAGAA
  TACCCCAGCTCCAGCACCTCGCACCGCCTAGATGCCCTTGATCAGCTTCTGGAAGAGGCCAAAGTAACCT
  CAGGCTTGGTGAAACCTGTTTCTTGCTTCTCTCCTGGGCCCACTGCCTCCCCGCTGCTGCCTGCCTCTGC
  CCCAGCCAGACCTCTCCTGCCCTTCTCCACTGGGCCCGGAAGCCCTCTCTTCCAGTCCCAAGGCAGCCCT
  CAGAAAGGCCCAGAGCTCTCCTTGGCCAGTGTCCATGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCC
  TTCCCGTGACAGCCTATGATAAAAATGGCTTCCGCATCCTTTTCCACTTTGCTAAGGAGTGTCCGCCAGG
  ACGGCCTGATGTGCTGGTGGTGGTGGTTTCCATGCTGAATACGGCTCCGTTGCCAGTCAAGAGCATAGTG
  CTGCAGGCTGCAGTGCCCAAGTCAATGAAAGTGAAGTTGCAGCCACCCTCTGGGACAGAACTTTCCCCAT
  TTAGTCCTATCCAGCCACCTGCAGCCATCACCCAGGTCATGCTGCTGGCCAATCCAATGAAGGAAAAGGT
  GCGGCTTCGATATAAACTGACCTTTGCTTTGGGGGAGCAGCTGAGCACAGAACTGGGTGAAGTGGACCAG
  TTTCCTCCTGTGGAGCAGTGGGGGAACCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252067
• Insert Size: 1923 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252067.1, NP_001238996.1
• RefSeq Size: 3469 bp
• RefSeq ORF: 1923 bp
• Locus ID: 260302
• Cytogenetics: 11 E2
• Gene Summary: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.