Capn5 (NM_001301250) Mouse Untagged Clone

CAT#: MC228548

Capn5 (untagged) - Mouse calpain 5 (Capn5), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Capn5
• Synonyms: nCL-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228548 representing NM_001301250
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCTGCGCGAAGGCCTATGAGGACCAGAACTACTCGGCGCTGAAGCGGGCCTGCCTGCGCAAGA
  AGGTGCTGTTCGAGGATCCCCTCTTCCCTGCCACCGACGACTCCCTTTACTATAAGGGCACCCCAGGGCC
  CACAGTCAGGTGGAAGCGGCCTAAGGATATCTGCGACGATCCCCGGCTCTTCGTAGATGGCATCAGCTCC
  CATGACCTGCACCAGGGCCAGGTGGGCAACTGCTGGTTTGTGGCTGCCTGCTCATCACTGGCCTCCCGAG
  AGTCACTCTGGCAGAAGGTCATCCCAGACTGGAAGGAGCAGGAATGGAACCCCGAGAAGCCTGACAGCTA
  TGCTGGCATCTTCCACTTCAACTTCTGGCGCTTTGGGGAGTGGGTGGACGTAATCGTCGATGACCGGCTG
  CCCACAGTCAACAACCAGCTCATTTACTGCCATTCCAACTCCAAAAATGAGTTCTGGTGTGCCCTGGTGG
  AGAAGGCCTATGCCAAGCTGGCCGGCTGTTACCAGGCCCTGGACGGAGGCAACACGGCCGATGCATTGGT
  GGATTTCACAGGTGGTGTTTCTGAACCCATTGACCTGACCGAGGGGGACTTGGCCACTGACGAGGCTAAG
  AGGAATCAGCTCTTTGAGCGAGTGCTGAAGGTGCACAGCAGAGGCGGGCTCATCAGTGCCTCCATCAAGG
  CTGTGACAGCAGCTGACATGGAGGCCCGCCTGGCATGTGGCCTGGTGAAGGGCCATGCATACGCTGTCAC
  CGATGTGCGCAAGGTGCGCCTGGGCCATGGCCTGCTGGCCTTCTTCAAGTCAGAGAAGCTTGATATGATC
  CGTCTGAGGAACCCCTGGGGCGAGCGGGAGTGGACGGGGCCCTGGAGTGACACGTCAGAGGAATGGCAGA
  AAGTGAGCAAGAGTGAGAGGGAGAAGATGGGCGTGACCGTGCAGGATGATGGGGAATTCTGGATGACCTT
  TGAGGACATGTGCCGGTACTTTACTGACATCATTAAATGCCGCCTGATTAACACGTCCTACCTGAGCATC
  CATAAGACATGGGAGGAGGCCCGGCTGCATGGTGCCTGGACGAGACATGAGGACCCACAGCAGAACCGCA
  GTGGAGGCTGCATCAACCACAAGGACACTTTCTTCCAGAACCCACAGTACGTATTTGAAGTCAAGAAGCC
  AGAAGATGAAGTGTTGATCAGTATCCAGCAGCGGCCGAAGCGCTCAACTCGCCGGGAGGGCAAAGGCGAG
  AATCTGGCCATTGGCTTCGACATCTATAAGGTGGAAGAGAACCGCCAATACCGTATGCACAGCCTACAGC
  ATAAGGCCGCCAGCTCCATCTACATCAATTCCCGCAGCGTTTTTTTGAGGACAGAGCTGCCCGAGGGCCG
  CTACGTTATCATCCCTACCACCTTTGAGCCAGGCCACACTGGCGAGTTCCTGCTCCGAGTCTTCACAGAT
  GTCCCCTCCAACTGCCGGGAACTACGCCTGGATGAGCCCCCTCGGACCTGTTGGAGTTCCCTCTGTGGCT
  ACCCTCAGCAGGTGGCCCAGGTACATGTCCTGGGGGCTGCTGGCCTCAAGGACTCCCCAACAGGAGCAAA
  CTCATATGTGATCATCAAGTGTGAGGGCGAAAAGGTTCGCTCAGCTGTGCAGAGAGGGACCTCGACACCA
  GAGTACAATGTAAAAGGCATCTTCTATCGCAAGAAGCTGGCTCAGCCTATCACCGTGCAGGTTTGGAATC
  ACCGAGTCCTGAAGGATGAATTCCTGGGCCAGGTGCACCTGAAGACTGCCCCGGATGACCTGCAGGACCT
  CCACACCCTCCATCTCCAGGACCGCAGTAGCCGGCAGCCCAGTGACCTGCCAGGCATTGTAGCTGTGCGA
  GTCCTCTGCAGTGCCTCTCTCACGGCTGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301250
• Insert Size: 1923 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301250.1, NP_001288179.1
• RefSeq Size: 4351 bp
• RefSeq ORF: 1923 bp
• Locus ID: 12337
• UniProt ID: O08688
• Cytogenetics: 7 E1
• Gene Summary: The protein encoded by this gene is a member of the calpain family of proteins. Unlike many members of the calpain gene family, this gene lacks a calmodulin-like domain, required for calcium binding. Mouse models for Huntington's disease displayed increased levels of the protein encoded by this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and represents use of an alternate promoter, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.