Racgap1 (NM_001253809) Mouse Untagged Clone

CAT#: MC228520

Racgap1 (untagged) - Mouse Rac GTPase-activating protein 1 (Racgap1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Racgap1
• Synonyms: AI227039; AI327394; Band25; gtl11; GTPase; MgcRacGAP; mKIAA1478
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228520 representing NM_001253809
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACTACAATGGTGAATTTGTGGACTCTGTTTGAGCAGCTTGTGCGCCGGATGGAGATTATCAATG
  AAGGAAATGAAAGCATTGAATTCATCCAGGTTGTGAAGGACTTCGAGGACTTCCGAAAGAAGTATCAAAG
  AACCAACCAGGAGCTGGAGAAATTCAAAGACCTATTGTTGAAAGCAGAGACTGGGCGGAGCGCCCTGGAC
  GTGAAGCTGAAGCATGCCCGTAATCAAGTGGACGTGGAGATCAAGCGGAGGCAGCGCGCTGAGGCAGAGT
  GTGCAAAGCTGGAACAACAGATTCAGCTGATTCGAGACATACTCATGTGTGACACATCTGGCAGTATTCA
  GCTGAGCGAGGAACAAAAATCAGCTCTCGCTTTCCTCAACCGAGGCCAAGCATCCAGTGGCCACGCCGGC
  AACAATAGACTGTCAACGATTGATGAATCTGGTTCCATTTTATCAGATATCAGCTTTGACAAGACTGATG
  AATCACTGGACTGGGATTCTTCTTTGGTGAAGAATTTCAAAATGAAGAAACGAGAGAAGAGGCGCTCCAA
  CAGTAGACAGTTCATCGACGGCCCTCCTGGGCCTGTGAAGAAAACTTGTTCCATTGGCTCTACAGTAGAC
  CAGGCAAATGAATCAATAGTTGCAAAAACTACAGTGACTGTTCCCAGTGATGGGGGACCCATTGAAGCTG
  TGTCTACTATTGAGACATTGCCGTCCTGGACCAGGAGTCGCGGGAAGTCAGGACCTTTACAACCTGTGAA
  CAGTGACTCCGCTCTGAACAGCAGGCCACTGGAGCCAAGAACTGACACAGACAATTTGGGCACACCTCAG
  AATACTGGAGGCATGCGCTTGCACGACTTCGTCTCAAAGACGGTTATTAAGCCTGAATCTTGTGTTCCGT
  GTGGAAAGCGGATCAAGTTTGGCAAGCTGTCTCTGAAGTGTCGAGACTGTCGTTTGGTCTCCCATCCAGA
  ATGTCGGGACCGATGTCCCCTTCCCTGCATCCCCCCCCTGGTGGGGACACCGGTTAAGATTGGAGAGGGC
  ATGCTGGCCGACTTCGTGTCGCAGGCTTCTCCCATGATCCCTGCCATTGTCGTCAGCTGTGTCAATGAGA
  TCGAGCAGCGAGGCCTGACTGAGGCAGGCTTGTACAGGATCTCAGGCTGTGACCGCACAGTGAAAGAACT
  GAAAGAAAAATTCCTTAAGGTGAAAACTGTGCCCCTCCTCAGCAAAGTGGACGATATCCACGTCATCTGC
  AGCCTCCTGAAGGACTTCCTGCGCAACCTCAAAGAGCCCCTCCTGACCTTCTGGCTGAGCAAAGCCTTCA
  TGGAGGCAGCAGAGATAACAGATGAAGACAACAGCACAGCCGCCATGTACCAGGCTGTCAGTGAGCTGCC
  CCAGGCCAACAGGGACACGCTAGCCTTCCTTATGATCCACCTACAGAGAGTGTCTCAGAGTCCAGACACT
  AAGATGGATATTGCCAATCTAGCTAAAGTCTTTGGCCCTACAATAGTTGCCCATACTGTGCCCAATCCAG
  ATCCAGTGACAATGTTCCAGGACATCAAACGTCAGCTCAAGGTGGTGGAGCGCCTACTCTCTCTCCCCTT
  GGAGTACTGGAATCAGTTCATGATGGTGGACCAAGAGAACATAGACAGCCAGCGAGGCAATGGAAACTCA
  ACACCACGCACACCAGACGTTAAAGTGAGCTTACTGGGGCCTGTGACCACTCCTGAATTCCAGCTTGTCA
  AGACTCCTTTATCAAGTTCCCTGTCACAGAGGTTGTACAACCTCTCCAAGAGCACACCCAGATTTGGGAA
  CAAGAGCAAGTCTGCCACCAACTTAGGTCAACAGGGCAAATTTTTCCCTGCTCCGTACCTCAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253809
• Insert Size: 1887 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253809.1, NP_001240738.1
• RefSeq Size: 2930 bp
• RefSeq ORF: 1887 bp
• Locus ID: 26934
• UniProt ID: Q9WVM1
• Cytogenetics: 15 F1
• Gene Summary: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks a segment in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein.