Tktl2 (NM_001271574) Mouse Untagged Clone

CAT#: MC228513

Tktl2 (untagged) - Mouse transketolase-like 2 (Tktl2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tktl2
• Synonyms: 4933401I19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228513 representing NM_001271574
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGGCCAGAGATGCCAAGCTGGAGTCGGACACCTTTCAGGTGCTTCAGGACGTGGCCAATCGCC
  TGCGAATCCACTCAATCAGGGCCACGTGTGCCTGCAGCTCCGGACATCCCACGTCGTGCTGTAGTGTGGC
  AGAGATCATGGCTGTGCTCTTCTTCCATACCATGAGGTACAAACAGGCAGACCCAGAACACCCGGACAAT
  GACCGCTTTGTCCTCTCCAAGGGCCACGCCGCGCCCATCCTCTATGCGGTTTGGGTGGAGGTGGGCCGTA
  TCTGTGAATCGGACTTGCTGAACTTGCGAAAAATTCACTGCGACCTGGAGGGTCATCCCACCCCACGACT
  GTCGTTTGTTGATGTGGCAACAGGGTCCCTTGGGCAAGGACTGGGTGCTGCCTGTGGAATGGCTTACACC
  GGCAAATACTTTGACAAGGCCAGCTACCGCGTGTTCTGCCTCATGGGGGATGGTGAGTCCTCGGAAGGTT
  CTGTCTGGGAGGCCTTGGCCTTTGCTTCCCACTACAACTTGGACAATCTCGTGGCAATCTTTGACGTGAA
  CCGCTTGGGACAAAGCGGCACAGCACCCCTAGAGCACTGTACAGCCGTTTATGAGAAGCGCTGCCAAGCG
  TTTGGGTGGAATACTTACGTGGTGGATGGTCATGATGTTGAAGCTCTCTGCCAGGCCTTTTGGAAAGCAG
  CTCAAGTCAAGAATAAGCCTACTGCCCTGATTGCCAAGACCTTCAAGGGTAGAGGTATTCCAAATGTCGA
  GGATGCGGAAAATTGGCATGGAAAGCCTATGCCAAAAGACAGAGCTGATGGGATTGTCAAATTAATTGAG
  AATCGGATACAGACAAACCGAAATCTCACACCGAAACCCCCCATTGAAGACTCGCCCAGGATCAGCATGT
  CCAATACAAAAATGACCTCTCTGCCTGTCTACAAACTGGGAGACATGATAGCTACTCGTGAAGCATACGG
  CTTGGCTCTGGCTAAATTGGGCCAATCTAACCAAAGAGTTATCGTTCTAGATGGCGACACAAAAAACTCT
  ACCTTTTCAGAAGTATTCAAGAAAGAGCACCCTGAGCGTTTCATAGAGTGCTTTATCGCTGAGCAAAACA
  TGGTAAGTGTGGCCCTGGGGTGTGCCACACGAGGACGAACTATTGCTTTCGTTAGTACCTTTGCTGCCTT
  CCTGACCCGAGCCTTTGATCAGATCCGAATGGGAGCCATCTCTCAAACCAACATCAACTTCGTTGGTTCC
  CACTGCGGGGTGTCTGTTGGAGAAGACGGACCTTCCCAGATGGCCCTGGAGGACCTAGCCATGTTCAGAA
  GCATCCCCAACTGCACTGTTTTCTATCCAAGTGATGCTGTCTCGACAGAACACGCTGTTTATCTGGCAGC
  CAACACGAAGGGAATGTGCTTCATTCGTACCACTCGGCCAAAAACAGCAGTTATTTATACAGCTGAAGAA
  AACTTTGTGATTGGACAGGCCAAGGTGATCCGCCAGAGTGCAGTCGACAAGGTGACAGTTATTGGAGCAG
  GGGTTACTCTGCATGAAGCCTTAGTAGCTGCTGAGGAGCTTTCTCAACAAGGGATTTTTATTCGTGTCAT
  TGACCTGTTCACTATCAAACCTCTGGATGCTGTCACCATTATCCAAAGTGCCAAAGCCACAGGTGGCCAG
  ATCATCACTGTGGAAGATCACTACCGAGAAGGTGGCATTGGTGAAGCTGTGTGTGCAGCCATCTCTAGAG
  AGCCTGACATAGTCGTTCGTCAACTTGCAGTAACAGAAGTACCTCGAAGCGGGAAACCTAGCGAGCTACT
  GGATATGTTTGGAATCAGTGCCAGACACATTATAGCAGCTGTGAAAGATACCGTAATGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271574
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271574.1, NP_001258503.1
• RefSeq Size: 6368 bp
• RefSeq ORF: 1884 bp
• Locus ID: 74419
• UniProt ID: Q9D4D4
• Cytogenetics: 8 B3.1
• Gene Summary: Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon structure in the 3' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.