Prickle3 (NM_001290624) Mouse Untagged Clone

CAT#: MC228509

Prickle3 (untagged) - Mouse prickle homolog 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prickle3
• Synonyms: Lmo6; Pk3; Sfc16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228509 representing NM_001290624
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGCGCGTGGGTCCCGGAGGCGCCGCTCTGGGCGTGCGCCTCCAGAGGCAGAGGACCCTGCCCGCG
  GCCAGCCTTGCAATTCCTGCAGGGAACAGTGCCCAGGCTTCCTGCTGCATGGCTGGAGAAAGATCTGCCA
  GCACTGCAAATGCCCGCGGGAGGAGCATGCTGTACGCACTGTACCTGTGGACCTAGAACGCATCATGTGC
  CGGCTCATCTCAGACTTCCAGCGCCACTCCATTTCCGATGACGACTCAGGCTGTGCCTCAGAGGAGTATG
  CCTGGGTACCCCCTGGCCTTAAGCCGGAGCAGGTTTACCAGTTTTTCAGTTGCCTTCCAGAGGACAAGGT
  CCCCTATGTCAACAGTCCTGGGGAGAAATACAGAATAAAGCAGCTACTGCACCAGCTACCCCCACACGAC
  AGTGAGGCACAGTACTGCACGGCCCTGGAAGAGGAGGAGAAGAAAGAGTTGCGAGCCTTCAGCCAGCAGA
  GGAAGCGGGAGAATCTGGGACGAGCCACCGTTCGAATCTTCCCAGTGACCATTACTGGGGCCATCTGCGA
  GGAGTGTGGCAAGCAGATCGGCGGTGGGGACATTGCAGTCTTTGCCAGCCGCGCAGGCCTTGGTGCCTGT
  TGGCACCCTCAGTGCTTTGTGTGTACCACGTGCCAGGAGCTGCTGGTTGACCTCATCTACTTCTATCATG
  CTGGCAAGGTCTACTGTGGGCGCCACCATGCTGAGTGTCTGCGTCCTCGCTGCCAAGCGTGTGATGAGAT
  CATCTTCTCCCCTGAATGTACGGAGGCGGAGGGGCGACACTGGCACATGGGTCACTTCTGCTGTTTCGAG
  TGTGAAGCTTCGTTAGGAGGGCAGCGCTATGTCATGCGTCAGAGCCGCCCCCACTGCTGTGCCTGCTATG
  AGGCCCGCCATGCGGAATACTGTGATGGCTGTGGGGAACACATCGGCCTGGACCAGGGCCAGATGGCTTA
  TGAGGGCCAACACTGGCACGCTTCAGATCGCTGCTTCTGCTGTAGCCGTTGCAGTCGACCTCTTCTGGGC
  CGCCCTTTCCTGCCACGCCGAGGCCTGATCTTCTGCTCACGAGCCTGCAGCCTTGGGTCTGAGACCACTG
  CTCCAGGGCCCGGCCGACGCAGCTGGAGCGCCGGCACCGTCACCACACCGCTCACAACTTCTACAGCTTC
  TTTCTCAGCTACGGAGGGGACATCAGAGACAGCCAGCAAAGGCACCTGTACCAAGGCAGAGCCTGCTGCA
  GGCCCTGAAGAGCCCTCCCACTTTCTGAGAGGGGCCCCCCACCGCCATTCTATGCCTGAACTGGGACTCC
  GCAGTGCTCCTGAGCCACCCACAGAATCCCCTGGCCATCCTGCCCCGCATCCAGATGATAATGCCTTTGG
  TCGCCAAAGTACACCTCGTGTCAGCTTCCGAGACCCTCTGGTGTCTGAAGGAGGTCCACGAAGGACCCTT
  AGTGCACCTCCAGCCCAGCGCCGTAGACCACGAAGTCCCCCACCCAGGACCCCCAGCTGCCACCACCACC
  ACCATCATCGCCGCCGGCGCCAGCGGCACCGCCGCCGAGGGAGCCACCATCACCACCACCATCCTGGCAG
  ACATGGCCACCATCGCTGTGACTTGGGATCAGGTTCAGATTCAGGATCTTGCTCCAGCTCACCCTCCAGC
  CCCAGTTCAGAGTCTTCTGAGGATGATGGCTTCTTCCTGGGGGAACGTATCCCACTGCCCCCTCACCTGT
  GCAGGCCCAGGACCACTCAGGACACCTCAACCGAAACCTTTAACTCCCCAGCCCAGCCACTTGTCCAGGA
  GTCTCACCCCGTGATGCCTCGCCAGACCCGGGACAAGAACTGCATCGTGGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290624
• Insert Size: 1875 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290624.1, NP_001277553.1
• RefSeq Size: 2410 bp
• RefSeq ORF: 1875 bp
• Locus ID: 54630
• UniProt ID: Q80VL3
• Cytogenetics: X 3.45 cM
• Gene Summary: Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation (By similarity). PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module (By similarity). Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions (By similarity). Involved in the organization of the basal body (By similarity). Involved in cilia growth and positioning (By similarity).[UniProtKB/Swiss-Prot Function]