Slc7a1 (NM_001301424) Mouse Untagged Clone

CAT#: MC228503

Slc7a1 (untagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (Slc7a1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc7a1
• Synonyms: 4831426K01Rik; AI447493; Atrc-1; Atrc1; Cat1; mCAT-1; Rec-1; Rev-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228503 representing NM_001301424
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCAAAAACCTGCTCGGTCTGGGCCAGCAGATGCTGCGCCGGAAGGTGGTGGACTGCAGCCGGG
  AGGAGAGCCGGCTGTCCCGCTGCCTCAACACCTATGACCTGGTAGCTCTTGGGGTGGGCAGCACCTTGGG
  CGCTGGTGTCTATGTCCTAGCCGGTGCCGTGGCCCGTGAAAATGCTGGCCCTGCCATCGTCATCTCCTTC
  TTGATTGCTGCTCTCGCCTCCGTGCTGGCCGGCCTGTGCTACGGCGAGTTTGGTGCCCGTGTCCCCAAGA
  CGGGCTCAGCCTACCTCTACAGCTACGTGACGGTGGGGGAGCTTTGGGCCTTCATCACTGGCTGGAACCT
  GATTCTCTCCTACATCATCGGTACTTCAAGCGTGGCAAGAGCCTGGAGTGCGACTTTTGACGAGCTGATA
  GGCAAGCCCATCGGAGAGTTCTCACGTCAGCACATGGCCCTGAATGCTCCTGGGGTGCTGGCCCAAACCC
  CGGACATATTTGCTGTGATTATAATTATCATCTTAACAGGACTGTTAACTCTTGGCGTGAAGGAGTCAGC
  CATGGTCAACAAAATTTTCACCTGTATCAATGTCCTGGTCTTGTGCTTCATCGTGGTGTCCGGGTTCGTG
  AAAGGCTCCATTAAAAACTGGCAGCTCACGGAGAAAAATTTCTCCTGTAACAACAACGACACAAACGTGA
  AATACGGTGAGGGAGGGTTTATGCCCTTTGGATTCTCTGGTGTCCTGTCAGGGGCAGCGACCTGCTTTTA
  TGCCTTCGTGGGCTTTGACTGCATCGCCACCACAGGGGAAGAAGTCAAGAACCCCCAGAAGGCCATTCCT
  GTGGGCATCGTGGCGTCCCTCCTCATTTGCTTCATAGCGTACTTTGGCGTGTCCGCCGCTCTCACGCTCA
  TGATGCCTTACTTCTGCCTGGACATCGACAGCCCGCTGCCTGGTGCCTTCAAGCACCAGGGCTGGGAAGA
  AGCTAAGTACGCAGTGGCCATTGGCTCTCTCTGCGCACTTTCCACCAGTCTCCTAGGCTCCATGTTTCCC
  ATGCCCCGAGTTATCTATGCCATGGCTGAAGATGGACTACTGTTTAAATTTTTGGCCAAAATCAACAATA
  GGACCAAAACACCCGTAATCGCCACTGTGACCTCAGGCGCCATTGCTGCTGTGATGGCCTTCCTCTTTGA
  ACTGAAGGACCTGGTGGACCTCATGTCCATTGGCACTCTCCTGGCTTACTCTTTGGTGGCTGCCTGTGTT
  TTGGTCTTACGGTACCAGCCAGAACAACCTAATCTGGTATACCAGATGGCCAGAACCACCGAGGAGCTAG
  ATCGAGTAGATCAGAATGAGCTGGTCAGTGCCAGTGAATCACAGACAGGCTTTTTACCGGTAGCCGAGAA
  GTTTTCTCTGAAATCCATCCTCTCACCCAAGAACGTGGAGCCCTCCAAATTCTCAGGGCTAATTGTGAAC
  ATTTCAGCCGGCCTCCTAGCCGCTCTTATCATCACCGTGTGCATTGTGGCCGTGCTTGGAAGAGAGGCCC
  TGGCCGAAGGGACACTGTGGGCAGTCTTTGTAATGACAGGGTCAGTCCTCCTCTGCATGCTGGTGACAGG
  CATCATCTGGAGACAGCCTGAGAGCAAGACCAAGCTCTCATTTAAGGTACCCTTTGTCCCCGTACTTCCT
  GTCTTGAGCATCTTCGTGAACATCTATCTCATGATGCAGCTGGACCAGGGCACGTGGGTCCGGTTTGCAG
  TGTGGATGCTGATAGGTTTCACCATCTATTTCGGTTATGGGATCTGGCACAGTGAGGAAGCGTCCCTGGC
  TGCTGGCCAGGCAAAGACTCCTGACAGCAACTTGGACCAGTGCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301424
• Insert Size: 1869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301424.1, NP_001288353.1
• RefSeq Size: 7079 bp
• RefSeq ORF: 1869 bp
• Locus ID: 11987
• UniProt ID: Q09143
• Cytogenetics: 5 88.48 cM
• Gene Summary: High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Both variants 1 and 2 encode the same protein.