Tsga10 (NM_001290720) Mouse Untagged Clone

CAT#: MC228499

Tsga10 (untagged) - Mouse testis specific 10 (Tsga10), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tsga10
• Synonyms: 4933432N21Rik; AI843383; Gm217; Mtsga10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228499 representing NM_001290720
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGAAATAGATCTAAGAGTCCGAGACGCCCATCGCCAACTTCCCGGGCTGCAAACTGTGATGTAG
  AGCTTTTGAAGTCAACTGCAAGAGACCGTGAAGAGCTAAAGTGTATGCTGGAAAAATATGAACGTCATTT
  GGCAGAAATCCAGGGCAACGTCAAGGTTCTCACATCTGAGAGAGACAAGACCTTCCTCCTTTATGAGCAG
  GCACAGGAAGAAATTGCTCGACTTCGACGAGAAATGATGAAAAGCTGTAAGTCTCCTAAGTCAACCACAG
  CACATGCTATTCTTCGTCGGGTAGAGACGGAGAGAGATGTAGCCTTCACTGACCTACGAAGAATGACCAC
  AGAGCGAGACAGTCTGAGAGAAAGGCTCAAGATTGCTCAAGAGACAGCGTTCAACGAGAAGGCTCACTTG
  GAACAGCGGATAGAGGAGCTGGAGTGCACAGTTCACAACCTTGATGATGAGCGCATGGAACAGATGGCAA
  ACATGACTTTGATGAAGGAAACCATAACCACTGTGGAAAAAGAAATGAAATCATTAGCAAGAAAGGCAAT
  GGACACCGAGAGTGAGCTTGGCAGACAAAAAGCAGAGAATAATTCTTTGAGACTTTTATATGAAAACACA
  GAAAAAGATCTCTCTGATACTCAGCGACATCTTGCTAAGAAAAAATATGAGCTACAGCTTACTCAGGAGA
  AAATTATGTGCTTGGATGAAAAAATTGATAATTTCACGAGGCAAAATATTGCACAGCGAGAAGAAATCAG
  CATTCTTGGTGCAACCCTCAATGACCTGGCTAAAGAAAAGGAATGCCTGCAAGCGTGTCTGGATAAAAAG
  TCTGAGAACATTGCATCCCTTGGCGAGAGCTTGGCAATGAAAGAAAAAACCATTTCAGGCATGAAGAATA
  TCATTGCTGAGATGGAACAGGCATCAAGACAGTCTACTGAAGCCCTCATTATGTGCGAACAAGATATTTC
  CAGGATGCGCCGGCAGTTGGACGAGACAAATGATGAGCTGGGTCAGATTGCCAGGGAGAGAGATATCTTG
  GCTCATGAGAATGACAATCTTCAAGAACAGTTTGCCAAAGTCAAACAAGAAAACCAGGCACTGTCCAAAA
  AACTGAATGATACTCATAACGAACTCAGTGACATAAAGCAGAAGGTCCAGGACACGAATCTGGAGGTTAA
  CAAGTTGAAGAACATATTAAAGTCTGAGGAATCTGAGAACCGGCAAATAATGGAACAACTCCGAAAAGCC
  AATGAAGATGCTGAAAACTGGGAAAATAAGGCCCGCCAGACAGAGGCAGAAAACAACACCCTCAAACTGG
  AACTTATCACTGCTGAAGCAGAGGGCAACAGATTAAAGGAAAAAGTTGATGCCCTTAACAGAGAGGTTGA
  GCAAATGGAGAATGAGCTGGACTCAGCGCGCTCTGAAATTGAACTGCTCCGGAGTCAGATGACAAACGAG
  AGGATCTCCATGCAGAATCTCGAGGCTCTGCTGGTGGCCAACCGGGACAAAGAGTACCAGTCTCAGATAG
  CACTGCAGGAAAAGGAGTCTGAGATCCAGTTGCTGAAGGAGCACCTCTGCCTGGCTGAGAACAAAATGGC
  CATCCAGAGCAGAGACGTGGCACAGTTCAGAAATGTTGTAACGCAGTTAGAAGCAGATTTGGACATTACC
  AAAAGGCAACTCGGGACAGAACGTTTTGAAAGGGAAAGGGCTGTCCAAGAACTTCGCCGCCAGAATTACT
  CAAGCAATGCCTATAATTTGGGTCCAATGAAGCCAAATACAAAATGCCACTCACCAGAGCGTGCTCACCA
  TCGATCTCCTGACCGAGGCCTCGACCGATCATTGGAAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290720
• Insert Size: 1863 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290720.1, NP_001277649.1
• RefSeq Size: 2706 bp
• RefSeq ORF: 1863 bp
• Locus ID: 211484
• UniProt ID: Q6NY15
• Cytogenetics: 1 B
• Gene Summary: Plays a role in spermatogenesis (PubMed:14585816). When overexpressed, prevents nuclear localization of HIF1A (PubMed:16777103).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 3' structure and 3' terminal exon, compared to variant 1. The encoded protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1.