Sass6 (NM_001289571) Mouse Untagged Clone

CAT#: MC228493

Sass6 (untagged) - Mouse spindle assembly 6 homolog (C. elegans) (Sass6), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sass6
• Synonyms: 2810453L12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228493 representing NM_001289571
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAGACTGCGAGGAGAGGATTTAGTCATCCGTCTGACTGATGATACAGATCCGTTTTTTCTGTATA
  ACCTTGTTATATCTGAGGAAGATTTTCAAAGTTTAAAATTGCAGCAAGGTCTCCTGGTGGACTTCTTAGC
  TTTCCCACAGAAGTTTATAGACCTCCTTCAGCAGTGTATGCAAGAACACGCGAAAGAGACTCCAAGGTTC
  CTGCTGCAGCTTCTTTCTTCAGCCACTCTTTTGGAGAACTCACCGGTCCTTTTAAACGTAGTGGAGACAA
  ATCCTTTTAAGCATCTTATTCACCTGTCACTAAAGCTTTTACCTGGAAATGATGTAGAAATAAAGAAATT
  TCTAGCAGGATGTTTGAAATGTAGCAAGGAAGAAAAATTATCACTGACTAGATCACTAGATGATGTTACC
  AGGCAACTGCACATTACACAAGAGACGTTGTCGGAAAAAATGCAAGAATTAGATAAGCTGCGGAGTGAAT
  GGGCCTCGCACACAGCATCGCTGACGAATAAGCACTCTCAGGAGTTAACAGCTGAGAAGGAGAAGGCCTT
  GCAGACCCAAGTTCAGTGCCAACAGCAGCACGAGCAACAGAAAAAGGAACTGGAAACCCTCCATCAACGG
  AATATCCACCAGCTACAAAGCAGATTGTCCGAGTTAGAGGCAGCTAATAAAGAGCTCACCGAGAGGAAGT
  ATAAAGGAGACTCGACTGTCCGAGAGCTAAAGGCGAAGCTGGCTGGCGTGGAAGAGGAGCTGCAGCGGGC
  CAAGCAAGAGGTCCTTTCTCTGAGAAGAGAGAATTGTACTCTGGATACTGAATGCCACGAGAAAGAAAAG
  CACATCAACCAGCTACAAACAAAAGTGGCCGTTTTAGAACAGGAGATCAAAGATAAAGACCAGCTTGTTC
  TGAGAACAAAAGAAGCATTCGATACAATCCAAGAGCAAAAGGTGGCTTTAGAAGAAAATGGTGAGAAAAA
  TCAGATACAACTGGGAAAACTTGAAGCTACAATAAAATCATTATCAGCAGAACTTCTAAAGGCAAATGAG
  ATCATCAAGAAGTTACAAGGAGATCTTAAAACTCTGATGGGTAAATTGAAACTGAAGAATACAGTTACTA
  TTCAGCAAGAAAAACTATTGGCTGAGAAAGAAGAAATGCTACAAAAGGAGCGAAAGGAATCACAGGACGC
  TGGGCAGTTTCTTCGTGCCAAAGAGCAAGAGGTATGCAGATTACAGGAACAATTAGAAACTACAGTTCAG
  AAACTCGAAGAAAGTAAACAACTTTTGAAAAATAATGAAAAATTAATCACGTGGCTAAACAAAGAGCTAA
  ATGAAAATCAGCTGGTAAGAAAACAGGACACATTGGGAACCTCTGCCACCCCACATTCTACTAGCAACAG
  CACCATCAGAAGTGGGCTCTCTCCCAATCTGAATGTGGTTGATAGACTAAATTACCCAAGCTGTGGAATT
  GGCTATCCTGTCTCCTCTGCATTGACATTCCAGAATGCTTTTCCTCATGTAGTAGCTGCCAAAAACACCA
  GCCACCCTATCTCTGGACCAAAGGTTCACTTTAACCTGCAGCTTACAAAACCAAGCGCTTCGATAGATGG
  GCAGCCGGGAGCCGCTGTTAACAGGCCTTGTTCAAATGATAAGGAAAATGGTGAAACTTTAGGATTGGAA
  TCCAAATACTTGAAGAGAAGAGAAGCTAGCATTCCTTTACGCGGACTTAGCCAGAATCTGTTGAGTGACT
  CAGACCATCAGAAAGACGGCATGCTGGGAGCGTTCCAGCTATCTTCCAAACCCACCGTTCTCCCCTCCTC
  GTCTTCAGCCTACTTCCCTGGGCAGTTACCAAGTAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289571
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289571.1, NP_001276500.1
• RefSeq Size: 4248 bp
• RefSeq ORF: 1860 bp
• Locus ID: 72776
• UniProt ID: Q80UK7
• Cytogenetics: 3 G1
• Gene Summary: Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication: required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells (PubMed:24240477). Required for the recruitment of STIL to the procentriole and for STIL-mediated centriole amplification (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon, that causes translation initiation at a downstream start codon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.