Itk (NM_001281968) Mouse Untagged Clone

CAT#: MC228486

Itk (untagged) - Mouse IL2 inducible T cell kinase (Itk), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itk
• Synonyms: Emt; Tcsk; Tsk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228486 representing NM_001281968
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACTTCATCCTCCTGGAAGAACAGCTGATCAAGAAGTCCCAACAGAAGAGAAGGACTTCTCCCT
  CGAATTTTAAAGTTCGTTTCTTTGTCTTAACGAAAGCCAGCCTGGCCTACTTTGAGGACCGCCACGGGAA
  GCGCACGTTGAAGGGCTCCATTGAACTCTCCAGAATCAAGTGTGTGGAGATTGTCAAGAGTGACATTAGC
  ATCCCGTGCCACTATAAATACCCTTTTCAGGTCGTGCATGACAACTATCTCCTGTATGTGTTTGCTCCAG
  ACTGTGAGAGTCGGCAGCGCTGGGTGCTGACCCTTAAAGAAGAAACGAGGAATAACAACAGCCTGGTATC
  CAAGTATCACCCTAATTTCTGGATGGATGGGCGGTGGAGGTGCTGCTCCCAGCTGGAGAAGCCTGCTGTA
  GGCTGTGCTCCCTACGACCCATCCAAGAATGCTTCAAAGAAGCCTCTTCCTCCTACTCCTGAAGACAACA
  GGCGGTCATTTCAGGAACCTGAAGAAACCCTGGTCATTGCCTTGTACGACTACCAAACCAACGACCCTCA
  GGAGCTCGCACTGCGGTGTGATGAAGAGTACTACCTGCTGGACAGCTCCGAGATCCACTGGTGGAGGGTT
  CAAGACAAAAATGGGCATGAAGGATATGCACCAAGCAGTTACCTGGTAGAAAAATCTCCAAATAACCTTG
  AAACCTATGAGTGGTACAATAAAAGCATCAGCCGCGACAAAGCTGAAAAACTTCTTTTGGACACAGGTAA
  AGAAGGAGCTTTCATGGTCCGAGATTCCAGGACACCCGGGACATACACAGTCTCTGTTTTCACCAAGGCC
  ATCATAAGTGAGAACCCCTGTATAAAACATTATCACATCAAAGAAACAAATGACAGCCCCAAGCGCTACT
  ACGTGGCTGAGAAGTATGTGTTTGACTCCATCCCTCTCCTCATCCAGTATCACCAGTACAATGGAGGAGG
  TTTGGTCACTCGACTCCGCTATCCAGTTTGCTCCTGGAGACAAAAAGCCCCTGTCACAGCAGGGCTAAGA
  TATGGGAAGTGGGTGATCCAACCCTCAGAGCTAACGTTCGTGCAGGAGATTGGCAGCGGGCAGTTTGGGC
  TGGTGCATCTCGGCTACTGGCTCAACAAGGACAAGGTGGCCATCAAGACCATTCAGGAAGGGGCGATGTC
  AGAAGAAGACTTTATCGAGGAGGCGGAAGTCATGATGAAACTCTCTCACCCCAAACTGGTGCAGCTCTAT
  GGGGTGTGCCTGGAGCAAGCCCCCATCTGCCTGGTGTTTGAGTTCATGGAGCACGGCTGCCTGTCGGATT
  ACCTTCGAAGTCAGCGGGGTCTCTTTGCTGCGGAGACCCTGCTGGGCATGTGCCTGGATGTGTGTGAGGG
  CATGGCCTACCTGGAAAAAGCTTGTGTCATCCACAGAGACCTGGCAGCCAGAAACTGTTTGGTGGGAGAA
  AACCAGGTCATCAAGGTGTCCGACTTTGGGATGACAAGATTTGTCCTTGATGATCAATATACCAGCTCCA
  CGGGCACCAAATTCCCAGTGAAGTGGGCATCCCCAGAGGTGTTCTCCTTTAGTCGCTATAGCAGCAAGTC
  AGATGTGTGGTCGTTTGGTGTACTGATGTGGGAAGTCTTCAGTGAAGGCAAAATCCCATACGAAAACCGA
  AGCAATTCCGAGGTCGTGGAAGATATCAGCACTGGCTTTCGGTTATACAAGCCCCGCCTGGCCTCCTGTC
  ATGTCTACCAGATCATGAATCATTGCTGGAAAGAGAAACCAGAAGACCGGCCACCCTTCTCCCAGCTGTT
  AAGCCAGCTGGCTGAAATCGCAGAAGCTGGGCTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281968
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281968.1, NP_001268897.1
• RefSeq Size: 4271 bp
• RefSeq ORF: 1857 bp
• Locus ID: 16428
• UniProt ID: Q03526
• Cytogenetics: 11 27.75 cM
• Gene Summary: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) uses two alternate in-frame splice sites in the central coding region, compared to variant 1. The encoded isoform (5) is shorter, compared to isoform 1.