Dync1i2 (NM_001198875) Mouse Untagged Clone

CAT#: MC228485

Dync1i2 (untagged) - Mouse dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dync1i2
• Synonyms: 3110079H08Rik; AW554389; Dncic2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228485 representing NM_001198875
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACAAAAGTGATTTAAAAGCTGAGTTGGAACGTAAGAAGCAACGGTTGGCCCAAATCAGAGAAG
  AAAAGAAGAGGAAAGAAGAGGAAAGGAAAAAAAAGGAAACTGATCAGAAGAAGGAAGCTGCTGTTTCTGT
  ACAAGAAGAGTCTGATCTTGAAAAAAAAAGAAGAGAAGCTGAGGCATTGCTTCAAAGCATGGGACTAACT
  ACGGACTCCCCTATTGTTTTTTCTGAGTACTGGGTCCCTCCTCCTATGTCTCCATCCTCCAAGTCGGTGA
  GCACGCCAAGTGAAGCTGGAAGCCAGGATTCTGGAGATGGCGCCGTGGGATCTAGACGAGGACCTATTAA
  ACTTGGAATGGCCAAAATTACTCAAGTTGACTTTCCCCCTCGAGAAATCGTCACATACACAAAGGAAACT
  CAGACCCCGGTTACAGCTCAACCCAAAGAAGATGAGGAGGAGGAAGATGATGTAGCAACTCCTAAACCTC
  CTGTTGAACCTGAAGAAGAGAAAACTTTGAAAAAAGACGAAGAGAATGATAGCAAAGCTCCACCTCATGA
  ACTAACTGAAGAAGAAAAGCAACAGATTTTACACTCAGAAGAATTTTTAAGTTTCTTTGACCATTCTACG
  AGAATTGTAGAAAGAGCCCTTTCTGAGCAGATTAACATCTTCTTTGACTACAGTGGGAGAGACTTGGAAG
  ACAAAGAAGGGGAGATTCAAGCAGGTGCTAAGCTGTCATTAAATCGACAGTTCTTTGATGAGCGTTGGTC
  AAAGCATCGAGTTGTTAGTTGTTTGGATTGGTCATCCCAGTATCCAGAATTACTTGTGGCTTCCTATAAT
  AACAATGAAGAGGCTCCTCATGAGCCTGATGGTGTGGCCCTCGTGTGGAATATGAAGTACAAAAAAACTA
  CCCCAGAGTATGTGTTCCACTGCCAGTCAGCTGTAATGTCTGCTACATTTGCAAAATTTCATCCGAATCT
  TGTTGTTGGAGGTACATATTCAGGCCAGATTGTGCTTTGGGATAACCGCAGCAATAAAAGAACTCCTGTC
  CAGAGAACGCCGCTGTCAGCTGCTGCACACACACACCCTGTCTACTGTGTAAATGTTGTTGGAACACAAA
  ATGCTCACAATCTGATTAGCATCTCTACTGATGGAAAAATTTGTTCCTGGAGTTTGGACATGCTTTCCCA
  TCCACAGGATAGCATGGAGTTGGTTCATAAACAGTCAAAGGCAGTAGCTGTAACATCTATGTCTTTCCCT
  GTTGGAGACGTCAACAACTTTGTTGTGGGCAGTGAAGAAGGTTCTGTGTATACAGCATGCCGCCATGGCA
  GCAAAGCTGGAATCAGTGAGATGTTTGAGGGACACCAGGGACCCATCACTGGAATCCACTGTCACGCGGC
  TGTTGGAGCAGTAGACTTCTCACATCTGTTTGTCACTTCGTCTTTTGACTGGACAGTTAAACTTTGGACA
  ACTAAGAATAACAAGCCTTTGTACTCATTTGAAGATAATTCAGACTATGTCTATGATGTTATGTGGTCAC
  CCACCCACCCAGCCCTGTTTGCCTGTGTGGACGGCATGGGGAGACTGGATCTATGGAATCTCAACAATGA
  CACAGAGGTACCAACTGCCAGCATTTCTGTGGAGGGCAACCCTGCTCTTAACCGTGTGAGATGGACCCAC
  TCAGGAAGAGAAATTGCCGTGGGTGATTCTGAAGGACAGATTGTCATCTATGATGTGGGAGAGCAGATCG
  CTGTTCCCCGAAATGATGAATGGGCAAGATTTGGCCGAACACTCGCAGAAATTAACGCAAACCGAGCTGA
  CGCAGAGGAGGAGGCAGCTACCCGAATCCCTGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198875
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198875.1, NP_001185804.1
• RefSeq Size: 2563 bp
• RefSeq ORF: 1857 bp
• Locus ID: 13427
• Cytogenetics: 2 42.38 cM
• Gene Summary: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4; also called 1a.F) lacks multiple exons in the 5' coding region, compared to variant 1. Variant 4 encodes a shorter isoform (4), compared to isoform 1.