Zap70 (NM_001289765) Mouse Untagged Clone

CAT#: MC228483

Zap70 (untagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zap70
• Synonyms: mrtle; mur; S; Srk; ZAP-; ZAP-70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228483 representing NM_001289765
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGATCCCGCGGCGCACCTGCCATTCTTCTATGGCAGCATCTCGCGGGCTGAGGCCGAGGAGCACC
  TGAAGCTGGCAGGCATGGCCGACGGGCTGTTCCTCCTGCGCCAGTGTTTGCGCTCCCTGGGCGGCTACGT
  GCTGTCGTTGGTGCACGACGTGCGCTTCCACCATTTCCCCATCGAGCGCCAACTCAACGGCACGTACGCC
  ATCGCGGGCGGGAAGGCGCACTGCGGCCCGGCCGAGCTCTGCCAGTTCTACTCTCAGGACCCCGACGGGC
  TGCCCTGCAACCTGCGTAAGCCGTGTAACCGGCCGCCCGGACTGGAGCCACAGCCCGGGGTCTTCGACTG
  CCTGCGTGATGCTATGGTGCGCGACTACGTGCGCCAGACCTGGAAGCTGGAGGGCGATGCACTAGAGCAG
  GCCATCATCAGCCAGGCCCCACAGGTGGAGAAGCTCATTGCTACCACAGCCCACGAGCGAATGCCCTGGT
  ATCACAGCAGCCTGACTCGTGAGGAGGCCGAGCGCAAACTCTATTCCGGCCAGCAGACCGACGGCAAGTT
  CCTGCTGAGGCCCCGGAAGGAGCAGGGCACATATGCACTGTCCCTGGTCTATGGGAAAACTGTATACCAC
  TATCTCATCAGCCAGGACAAGGCTGGCAAGTACTGCATCCCCGAAGGCACCAAGTTTGACACGCTCTGGC
  AGCTGGTGGAGTACCTGAAGCTGAAGGCGGATGGACTAATTTACCGCTTGAAGGAGGTCTGTCCCAACAG
  CAGCGCCAGCGCGGCAGTTGCTGCACCCACCCTCCCAGCCCACCCATCCACATTCACTCAGCCTCAGAGA
  CGAGTGGACACCCTGAACTCGGACGGATACACCCCTGAACCAGCACGCTTAGCGTCATCGACAGACAAGC
  CCCGGCCGATGCCCATGGACACAAGTGTGTACGAGAGTCCCTACAGCGACCCTGAGGAACTCAAAGACAA
  GAAGCTCTTCCTGAAGCGAGAGAATCTCCTCGTGGCGGACATCGAGCTTGGCTGTGGCAACTTTGGCTCC
  GTGCGCCAGGGAGTCTATCGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGGCA
  CAGAGAAGGCCGACAAAGATGAGATGATGCGAGAGGCCCAGATCATGCACCAGCTCGACAACCCCTACAT
  CGTGCGGCTCATCGGCGTGTGCCAGGCAGAAGCACTCATGCTGGTCATGGAGATGGCGGGAGGCGGGCCC
  CTGCACAAGTTCCTGCTGGGAAAGAAGGAGGAGATCCCTGTGAGCAATGTGGCTGAACTGCTGCACCAGG
  TGGCCATGGGCATGAAGTATTTGGAGGAGAAAAACTTTGTGCACCGCGACCTGGCAGCCCGCAATGTTCT
  ACTGGTCAATCGGCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAAGCCCTGGGTGCTGACGACAGC
  TATTACACAGCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGA
  AGTTCTCCAGCCGCAGTGACGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAGAA
  GCCCTACAAGAAAATGAAGGGCCCCGAGGTCCTGGACTTCATCAAGCAGGGTAAGAGGATGGAATGTCCG
  CCGGAGTGTCCTCCTGAGATGTATGCACTTATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCCCCG
  ACTTCCTGACTGTGGAACAACGTATGCGGAACTATTACTACAGCCTGGCCAGCCGGGCCGAGGGACCCCC
  ACAGTGTGAACAGGTGGCCGAGGCTGCATGTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289765
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289765.1, NP_001276694.1
• RefSeq Size: 2290 bp
• RefSeq ORF: 1857 bp
• Locus ID: 22637
• UniProt ID: P43404
• Cytogenetics: 1 15.41 cM
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 4. The encoded isoform (a) has a shorter N-terminus compared to isoform b. Variants 1 and 3 encode the same protein.