Rasal1 (NM_001281999) Mouse Untagged Clone

CAT#: MC228481

Rasal1 (untagged) - Mouse RAS protein activator like 1 (GAP1 like) (Rasal1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rasal1
• Synonyms: MRASAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228481 representing NM_001281999
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGTTGGAGCTGCGGGAGGCTCCGGGGACCACGTCCCCGCTGCGAGTGGAACTCTGGGATTGGGA
  CATGGTGGGCAAGAATGACTTCCTGGGTGGAGTTCACCCCACAGACCCTGCAGCAGAAGCCACCTAATGG
  CTGGTTCCGGCTCCTGCCCTTTCCTAGAGCTGAGGATTCTGGGGGGAGCCTGGGTGCCCTGCGGCTGAAG
  GTGCGCCTCACTGAGGACCGGGTCCTGCCTTCCCAGTACTACCAGCCTCTCATGGAACTGCTTCTGGAGT
  CGGTGCAAGGGCCGGCTGAGGAGGACACCACCAGCCCCCTGGCTCTGCTAGAGGAGCTGGCCTCTGGGGA
  CTGTCGTCAGGACCTTGCCACCAAGCTGGTGAAGCTGTTCCTGGGCCGGGGCCTGGCCGGGCCCTTTCTA
  GATTATCTCACAAGGCGTGAGGTGGCTCGAACCAATGACCCCAACACCCTCTTCCGTTCTAACTCCTTGG
  CATCCAAGTCGATGGAACAGTTCATGAAGCTCGTGGGCATGCGCTACCTGCACGAGGTCCTGAGGCCAGT
  GATCAGCCGCGTCTTCGAGGAGAAGAAATATATGGAACTGGACCCTTGCAAGATGGACCTGAACCGCTCT
  CGGAGGATCTCCTTCAAGGGCACGCCCACAGAGGAGCAGGTACGGGAGACCAGCCTGGGGCTGCTGACCG
  GATACCTAGGGTCCGTTGTGGACGCCATCGTGAGCTCTACAGGGCGCTGCCCACTTGCCTTGCGCTTGGC
  CTTTAAGCAGCTCCAGCGGTGTGTGGAGAAGCGCTTCTCTGGGATAGAGCATCAGGATGTGAAGTACCTG
  GCCATCAGTGGCTTCCTCTTTCTGCGGTTCTTTGCGCCTGCCATCCTCACACCGAAACTGTTTGACCTCA
  GAGACCAGCACGCAGACCCCCAGACCAGCCGTTCCCTGCTGCTGCTCGCCAAGGCTGTGCAGAGCATTGG
  GAACCTGGGCCAGCAGCTGGGCCAGGGCAAGGAGCAGTGGCTAGCCCCGCTCCATCCCTTCCTGCTGCAG
  AGCATCTCACGTGTGAGGGACTTCCTGGACCAGCTGGTGGATGTGGACGAGGATGAGGAGGCCGGGGGTC
  CAGCCTGCGCCCTGGTCCAACCCTCAACCATTGTTCGAGAAGGCTTCCTGCTGAAGCGCAAGGAGGAGCC
  TGGAGGCCTGGCCACGCGCTTTGCCTTCAAGAAGCGCTACTTCCGGCTGAGTGGGCGGGACCTCTCCTAC
  TCCAAGACTCCGGAGTGGCAGGTTCACACATCCATCCCGCTGTCCTGCATCCGGGCTGTGGAGCATGTGG
  ACGAGGGTGCCTTTCAACTGCCACACGTCATGCAGGTGGTGACACAGGATGGCGCCGGGACGTCACACAC
  CACCTACCTCCAGTGTAAGAACGTGAATGACCTCAACCAATGGCTGTCAGCCCTGCGCAAAGCCAGTGCC
  CCCAACCCGGGCAAGCTGGTTGCCTGCCACCCTGGTGCCTTCCGCAGCGGGCGCTGGACCTGCTGCCTCC
  AGGCTGAGCGCTCAGCTGCTGGTTGTAGCCGCACACACTCAGCCATCACACTGGGAGACTGGAGTGACCC
  ACTGGATCCTGACGCCGAGGCCCAGGCAGTGTATCGCCAGCTGCTCCTGGGGCGGGACCAGCTCAGGCTG
  AAACTGCTGGAGGATTCCAGCCTGGACACAGAAGTGGACCCTGGGAGGGACTCCAGTGCCACAGACGGGC
  CCTGTGCTGAGGTTCTGGCCCAGCAGAGAGCAGCCACAACCCACCTGCTGCAGGTACTTGAAGACTTGGA
  GCAAGCCCACGAGGAGTTCCAGAAACGGGGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281999
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281999.1, NP_001268928.1
• RefSeq Size: 3259 bp
• RefSeq ORF: 1854 bp
• Locus ID: 19415
• Cytogenetics: 5 F
• Gene Summary: Probable inhibitory regulator of the Ras-cyclic AMP pathway. Plays a role in dendrite formation by melanocytes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, initiates translation at an alternate start codon, and uses an alternate splice site in its 5' coding region compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.