Sphk2 (NM_203280) Mouse Untagged Clone

CAT#: MC228480

Sphk2 (untagged) - Mouse sphingosine kinase 2 (Sphk2), transcript variant 1


  "NM_203280" in other vectors (1)

Reconstitution Protocol

USD 633.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "Sphk2"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Sphk2
Synonyms C76851; Sk2; Spk2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228480 representing NM_203280
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCACCACCACTACTGCCAGTGGCTGCCAGCACTCCAATCCTGCACGGCGAGTTTGGTTCCTACC
CGGCCAACGGCCCACGGTTTGCCCTCACCCTCACAACACAAGCCCTACACATACAGCGACTACGCCCAAA
GCCAGAAGCCCGGCCCCGAGATGGTCTAGTCTCTCTGGATGAGGTCTCGGGCTGTGGCACCCTGCAGAGC
CGTAGCCCCGAGGACACTGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGTCGAGGGGGCCGGC
GCAGAGCTACGCGGACCTTCCGGGCGGATGGGGCCACCACTTATGAGGAGAATCGTGCAGAGGCCCAGCG
CTGGGCCACTGCCCTCACGTGTCTCCTCCGAGGAGTGCCTCTGTCAGGGGACCAGGAAATCACCCCTGAA
TTGCTGCCCCGGAAGCCCAGGCTGCTCATATTGGTCAATCCCTTTGGGGGGCGGGGCCTGGCCTGGCAGC
GCTGTATGGACCACGTGGTGCCAATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACG
ACAGAACCATGCCCGTGAGCTGGTGCAGGGGTTAAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCT
GGAGACGGGCTGCTTTACGAGGTGCTGAATGGGCTCCTTGATCGGCCAGACTGGGAGGATGCCGTGCGGA
TGCCCATTGGTGTCCTCCCCTGTGGATCGGGCAATGCGCTAGCTGGGGCGGTGAACCATCATGGCGGGTT
TGAGCAGGTTGTCGGTGTTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGTGGTGGCAGCCATCCT
CTGGACCTGCTCTCTGTGACGCTAGCCTCGGGATCCCGCTGTTTTTCCTTCCTGTCAGTGGCCTGGGGAT
TCTTGTCAGATGTGGACATTCACAGTGAGCGCTTCAGGGCCCTGGGCAGCGCTCGATTCACACTGGGTGC
AGTGCTAGGCCTGGCCTCGTTGCATACCTACCGTGGACGCCTCTCCTACCTCCCCGCTACCACAGAACCA
GCCTTGCCCATCCCAGGCCACAGTCTGCCTCGAGCCAAGTCAGAACTAGTCTTGGCTCCAGCCCCAGCCC
CCGCCGCCACCCACTCGCCTCTACATCGATCTGTGTCTGACCTGCCCCTGCCCCTTCCCCAGCCTGCCTT
GGTCTCCCCTGGCTCCCCTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGA
GACTGGGGAGGAGCTGGGGATGCACCTCTGTCCCCAGACCCACTGCTGCCTTCATCCCCCAACGCTCTCA
AAACAGCTCAGCTTTCACCCATCGCTGAAGGGCCCCCAGAAATGCCAGCATCTTCGGGGTTCCTGCCTCC
CACCCACAGTGCCCCAGAAGCCTCTACCTGGGGCCCAGTGGACCACCTCCTCCCTCCCCTGGGCTCTCCA
CTGCCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTACTCATGTTGGGCATCTTGCCGAGCCACCTCT
GCGCAGACCTGATGGCAGCCCCACATGCACGCTTTGATGATGGCGTTGTGCACCTGTGTTGGGTGCGGAG
CGGCATCTCACGGGCTGCACTTCTACGCATTTTGCTGGCCATGGAGCATGGAAACCACTTCAGCCTGGGC
TGCCCCCATCTGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAACCACTCACGCCTCGTGGCCTGCTCA
CTGTAGATGGGGAGTTAGTGGAGTATGGGCCAATACAGGCGCAGGTGCACCCAGGTCTCGCCACGCTGCT
CACTGGGCCTGCAGGTCAAAAGCCACAAGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_203280
Insert Size 1854 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_203280.3, NP_975009.1
RefSeq Size 3552 bp
RefSeq ORF 1854 bp
Locus ID 56632
UniProt ID Q9JIA7
Cytogenetics 7 B3
Gene Summary This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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