Papss2 (NM_001201470) Mouse Untagged Clone

CAT#: MC228476

Papss2 (untagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Papss2
• Synonyms: 1810018P12Rik; AI159688; Atpsk2; AtpsU2; bm; Sk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228476 representing NM_001201470
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGGGTCAAGAAGCAGAAGACGGACCAGCAAAAATCCACCAATGTGGTCTACCAGGCCCATCATG
  TGAGCAGGAACAAGAGAGGACAAGTGGTTGGAACCAGGGGAGGATTCCGAGGATGTACCGTGTGGCTAAC
  AGGTCTCTCTGGTGCTGGGAAAACAACCATAAGCTTTGCTTTGGAAGAGTACCTTGTATCTCACGCCATC
  CCATGTTACTCTCTGGATGGGGACAATGTCCGTCATGGCCTTAATAAGAACCTGGGATTCTCTGCGGGGG
  ACCGAGAAGAGAATATCCGCCGGATCGCGGAGGTGGCCAGGCTCTTTGCCGACGCCGGCCTGGTTTGCAT
  CACCAGCTTTATCTCTCCTTTTGCAAAGGATCGTGAGAATGCCCGAAAAATCCACGAATCAGCAGGACTC
  CCGTTCTTTGAGATCTTTGTAGATGCGCCTTTAAATATCTGTGAAAGCCGAGACGTAAAAGGACTCTACA
  AACGAGCCCGAGCAGGAGAGATTAAAGGGTTTACAGGCATCGATTCTGACTATGAGAAACCTGAAACTCC
  AGAGTGTGTGCTGAAGACCAACCTGTCTTCAGTAAGCGACTGTGTGCAACAGGTGGTGGAACTTTTGCAG
  GAGCAGAACATTGTACCCCACACCACCATCAAAGGCATCCACGAACTCTTTGTGCCAGAAAACAAAGTCG
  ATCAAATCCGAGCTGAGGCAGAGACTCTCCCATCACTACCAATTACCAAGCTGGATCTGCAGTGGGTGCA
  GATTCTGAGTGAAGGCTGGGCCACTCCCCTCAAAGGCTTTATGCGGGAGAAGGAGTACTTGCAAACTCTA
  CACTTCGACACTCTACTGGACGATGGAGTCATCAACATGAGTATTCCCATTGTATTGCCCGTTTCTGCGG
  ATGACAAGGCACGGCTCGAAGGGTGCAGCAAATTTGCCTTGATGTACGAAGGTCGGAGGGTCGCTCTATT
  ACAGGACCCTGAATTCTATGAGCATAGGAAAGAGGAGCGCTGTTCTCGTGTGTGGGGAACAGCCACTGCA
  AAGCACCCCCATATCAAAATGGTGATGGAAAGTGGGGACTGGCTTGTTGGTGGAGACCTACAGGTGCTAG
  AGAGAATAAGGTGGGACGATGGGCTGGACCAATACCGCCTTACGCCTCTGGAGCTCAAACAGAAGTGTAA
  AGACATGAATGCTGATGCCGTGTTTGCATTCCAGTTGCGCAATCCTGTCCACAATGGTCATGCCCTCCTG
  ATGCAGGACACCCGCCGCAGGCTCCTGGAGAGGGGTTACAAGCACCCAGTCCTCCTGCTCCACCCTCTTG
  GGGGCTGGACCAAGGACGATGACGTACCTCTGGAATGGAGGATGAAACAGCATGCAGCTGTACTGGAGGA
  AAGGGTCCTGGATCCCAAGTCAACTATTGTTGCCATCTTTCCATCTCCTATGTTATATGCTGGTCCCACA
  GAGGTCCAGTGGCATTGCAGATGCCGGATGATTGCAGGAGCCAATTTCTACATTGTGGGTAGGGATCCCG
  CAGGAATGCCCCATCCTGAGACAAAGAAAGACCTATATGAACCCACCCACGGGGGCAAGGTCTTGAGTAT
  GGCCCCTGGCCTTACCTCTGTGGAAATAATTCCGTTCCGAGTGGCTGCCTACAATAAAATTAAAAAGGCC
  ATGGACTTTTATGATCCAGCAAGGCACGAGGAGTTTGACTTCATCTCAGGAACTCGCATGAGGAAGCTCG
  CCCGGGAAGGAGAAGATCCCCCAGATGGCTTCATGGCCCCGAAAGCGTGGAAAGTGTTGACAGATTACTA
  CAGGTCTCTGGAGAAGACCAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001201470
• Insert Size: 1845 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001201470.1, NP_001188399.1
• RefSeq Size: 3599 bp
• RefSeq ORF: 1845 bp
• Locus ID: 23972
• Cytogenetics: 19 27.46 cM
• Gene Summary: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have an important role in skeletogenesis during postnatal growth.[UniProtKB/Swiss-Prot Function]