Hs6st2 (NM_001290467) Mouse Untagged Clone

CAT#: MC228465

Hs6st2 (untagged) - Mouse heparan sulfate 6-O-sulfotransferase 2 (Hs6st2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hs6st2
• Synonyms: 6OST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228465 representing NM_001290467
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGCCTGCGTTTGCAGCCCGGGCGCTCGGGCCGCCACTGCAACCGGAGCAAGGAGCGCCCGCCC
  GCACCACCTGTCCCCGCCGGCATTCCAGAGTAGAGGCCGAATTGGCAGCAAGCCGGCCCGGGTCTGTTGC
  CGCCTCAGTCCGCGCCGGCCCTCCTAGGGGTGTGTCTCTCGGATTCAACTCCCCGCCGCTCCAGGACAAG
  CCCCCAAAGGCATTTTCTTCCTTGGCGGGAGCCTTGCGCGCCCCGCTCTTCGCGCTGCTGCCTCGAGGCC
  GCCGCAGGCGGATGCACGACCTCAGGCGACGCTGGGACCTGGGCTCCCTCTGCCGGGCCCTGCTCACTCG
  GGGCCTGGCAGCCGTGGGCCACTCGCTCAAGCACGTGCTCAGTGCGATCTTCTCCAAGATTTTCGGTCCC
  TTGGCCAGCGTCGGGAACATGGATGAGAAATCTAACAAGCTGCTGCTGGCTTTGGTGATGCTCTTCCTAT
  TTGCGGTGATCGTCCTCCAATACGTGTGCCCCGGCACAGAATGCCAGCTCCTCCGCCTGCAGGCGTTCAG
  CTCCCCGGTGCCGGATCCGTACCGCTCGGAGGATGAGAGTTCGGCCAGGTTTGTACCCCGCTACAATTTC
  AGCCGCGGCGATCTCCTGCGCAAGGTAGACTTCGACATCAAGGGCGATGACCTGATCGTGTTCCTGCACA
  TCCAGAAGACTGGGGGCACCACTTTTGGCCGTCACCTGGTGCGCAACATCCAGCTGGAGCAGCCATGTGA
  GTGCCGCGTGGGGCAGAAGAAATGCACTTGCCACCGGCCGGGTAAGAGGGAGACCTGGCTCTTCTCCAGG
  TTCTCCACCGGCTGGAGCTGCGGGCTGCATGCCGACTGGACCGAGCTCACCAGCTGCGTGCCGGCGGTGG
  TGGATGGCAAGCGCGACGCCAGGCTGAGACCTTCCAGGAACTTCCATTACATTACCATCCTGAGAGACCC
  AGTGTCACGGTACTTGAGTGAATGGAGGCATGTCCAGAGAGGAGCAACTTGGAAAGCATCCCTGCACGTC
  TGTGATGGAAGGCCCCCAACCTCTGAAGAGCTGCCCAGCTGCTACACCGGTGATGACTGGTCTGGATGCC
  CTCTCAAAGAGTTCATGGACTGTCCCTATAATCTGGCCAACAACCGCCAAGTTCGCATGCTATCTGACCT
  GACTCTAGTGGGATGCTACAACCTCTCTGTCATGCCTGAAAAGCAAAGAAACAAAGTCCTTCTGGAAAGT
  GCCAAATCCAATCTGAAGCACATGGCGTTCTTTGGCCTCACTGAGTTTCAGCGCAAGACCCAGTACCTGT
  TTGAGAAGACCTTCAACATGAACTTTATCTCGCCGTTTACCCAGTATAATACCACTAGGGCCTCTAGTGT
  TGAGATCAATGAGGAAATCCAAAAGCGTATTGAGGGACTGAATTTTCTGGATATGGAGTTGTACAGCTAT
  GCTAAAGACCTCTTTCTGCAAAGGTATCAGTTCATGAGGCAGAAAGAACATCAGGATGCCAGGCGGAAGC
  GTCAGGAGCAACGCAAATTTCTGAAGGGAAGGTTCCTTCAGACCCATTTCCAGAGTCAGAGTCAGGGTCA
  GAGCCAGAGCCAGAGTCCAGGTCAGAATCTGAGTCAGAATCCAAATCCTAACCCAAATCAGAACCTGACT
  CAGAACCTGAGTCACAATCTGACTCCGAGTTCAAATCCCAATTCGACCCAGAGGGAGAACCGGGGAAGTC
  AGAAGCAGGGCTCAGGCCAGGGACAAGGTGATAGCGGCACCAGCAATGGCACCAATGACTACATAGGGAG
  CGTAGAGACATGGCGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290467
• Insert Size: 1839 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290467.1, NP_001277396.1
• RefSeq Size: 4672 bp
• RefSeq ORF: 1839 bp
• Locus ID: 50786
• UniProt ID: Q80UW0
• Cytogenetics: X A5
• Gene Summary: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.