Tubgcp2 (NM_001286011) Mouse Untagged Clone

CAT#: MC228458

Tubgcp2 (untagged) - Mouse tubulin, gamma complex associated protein 2 (Tubgcp2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tubgcp2
• Synonyms: 1700022B05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228458 representing NM_001286011
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACCCTAGTAAAGGAATACCTGATTCTGGTCACACAGCTGGAACAGCTGCACAGGCAGGGCCTCC
  TCTCACTGCAGAAGCTCTGGTTCTACATCCAGCCAGCCATGCGCACCATCGACATCCTTGCCTCCCTTGC
  CACCTCAGTGGATAAAGGCGAGTGTGTGGGCGGGTCGACACTGAGCCTTCTTCATGATAGGAGCTTCAAC
  TACACCGGGGACAGCCAGGCGCAGGAGCTGTGCCTCTATCTGACCAAGGCAGCCAGTGCACCTTACTTCG
  AGATCCTGGAGAAGTGGATCTATAGGGGCATCATTCATGACCCATACAGTGAGTTCATGGTGGAGGAACA
  CGAGCTGCGGAAGGAGAAGATCCAGGAGGACTACAACGACAAGTACTGGGACCAGCGCTACACTGTGCTG
  CCGCAGCAGATCCCATCCTTCCTGCAGAAGGTAGCAGGCAAGATCCTCAGCACAGGAAAATACCTAAATG
  TGGTCAGAGAGTGTGGTCATGATGTCACCTGTCCCGTAGCCAAAGAAATCATCTATACCCTTAAAGAGCG
  GGCATATGTGGAGCAGATAGAGAAAGCGTTCAATTATGCCAGCAAAGTGCTGCTGGACTTCCTCATGGAG
  GAGAAGGAGCTGGTGGCCCACCTGAGGTCCATCAAGCGCTACTTCCTGATGGACCAGGGGGACTTCTTCG
  TGCATTTCATGGACCTGACTGAGGAGGAGCTCAGGAAGCCAGTGGAGGACATCATACTCACACGCCTGGA
  GGCCCTGTTGGAGCTGGCCCTGCGAATGAGCACTGCCAACACTGACCCCTTCAAAGACGACCTCAAGATT
  GAACTGATGCCTCACGACCTAATCACTCAGCTTTTGCGGGTTCTGGCCATCGAGACCAAGCAGGAAAAGG
  CAATGACCCATGCTGACCCCACGGAGCTCACACTGAGTGGTCTGGAGGCCTTCTCCTTTGACTACATGGT
  CAAATGGCCCCTGTCACTGATTATTAACAGGAAGGCACTCACCCGCTACCAGATGCTCTTCCGACACATG
  TTCTACTGCAAGCACGTGGAGCGGCAGCTCTGCAGTGTCTGGATCAGCAACAAGACGGCCAAGCAGCATG
  CACTGCACTCCGCCAAGTGGTTTGCTGGTGCTTTCACTCTGCGGCAGCGAATGCTCAACTTTGTCCAGAA
  TATTCAGTACTACATGATGTTTGAAGTGATGGAACCGACCTGGCACATCCTTGAGAAAAACCTGAAATCT
  GCCTCCAACATTGACGATGTCCTTGGCCACCACACAAGCTTCCTTGACAACTGCCTGAAGGACTGCATGC
  TGACCAACCCTGAGCTGCTGAAGGTTTTCTCCAAGCTCATGTCCGTATGCGTCATGTTTACCAACTGTAT
  GCAGAAATTTACACAGAGTATGAAACTGGACAGTGAGCTAGGCCGTCTGACACTGGATCAGGGTAGTGTG
  CAGGGGCCACCCACAGAGACTGAACGGATTGAGGATCGCCCCCGGAAGGAGCTTACCAGGAAGCATCTGT
  CTGAGCATGTGGATGCTCCTCAGCTGGCCTCTGGTTTCGAGGCCACCATCAACAACTTTGACAAAAACTT
  CTCTGCCCATCTTCTTGATTTGCTGGCCCGACTAAGTGTCTACAGCACCAGTGACTGCGAACATGGCATG
  GCCAGTGTCATCTCCAGACTAGATTTCAATGGTTTCTATGCTGAACGCCTGGAACGTCTGTCTGCAGAAA
  GGAGTCAGAAGGCTGCCCCCCAAGTGCCTGTCCCACGGGGACCCTCAGCACCAGCACCCAGGGTGGCCAT
  TCCTGCGCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286011
• Insert Size: 1833 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286011.1, NP_001272940.1
• RefSeq Size: 2925 bp
• RefSeq ORF: 1833 bp
• Locus ID: 74237
• Cytogenetics: 7 F4
• Gene Summary: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an alternate exon and uses a downstream start codon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.