Ncaph2 (NM_001271601) Mouse Untagged Clone

CAT#: MC228453

Ncaph2 (untagged) - Mouse non-SMC condensin II complex, subunit H2 (Ncaph2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncaph2
• Synonyms: D15Ertd785
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228453 representing NM_001271601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATGTGGAGGTGCGCTTTGCTCACCTCTTGCAGCCCATCCGGGATCTCACTAAGAACTGGGAGG
  TGGACGTGGCGGCACAGCTGGGCGAGTATCTGGAGGAGCTGGACCAGATCTGCATTTCTTTTGATGAAGG
  CAAAACCACAATGAACTTCATTGAGGCAGCACTGTTGATCCAGGGCTCAGCCTGTGTCTACAGTAAGAAG
  GTGGAGTACCTCTACTCGCTGGTCTACCAGGCTCTCGATTTTATTTCTGGCAAGAGGCGGGCCAAACAGC
  TCTCCTTAGTTCAGGAAGATGGGAGCAAGAAGACTGTCAACTCAGAGACTCCCTGTGAAACAGAGAATGA
  GTTCCTGTCACTGGACGACTTCCCTGACTCCCGGGCTAATGTGGATCTGAAAAATGATCAGGCATCCAGT
  GAGCTGCTTATCATACCCCTACTGCCCATGGCCCTGGTGGCCCCTGATGAAGTGGAAAAGAACAGCAGCC
  CCTTGTATAGCTGTCAGGGTGACATCTTGGCCAGCCGGAAGGATTTCAGGATGAACACGTGTATGCCTAA
  CCCCAGAGGCTGCTTTATGTTAGATCCAGTGGGAATGTGTCCTGTGGAGCCTGTGGTGCCCGTGGAGCCA
  TACCCCATGTCAAGGAGCCAGAAAGATCCTGAGGACGCTGAGGAGCAGCCCATGGAAGTGTCTAGGAACG
  GGAGTCCTGTTCCTGTACCCGACATCTCCCAAGAGCCAGATGGTCCAGCGCTCAGCGGTGGAGAGGAGGA
  TGCAGAGGATGGAGCAGAGCCCCTGGAGGTTGCTCTAGAGCCTGCAGAGCCAAGGACCTCACAGCAGAGT
  GCCATCTTGCCAAGGAGATACATGCTGCGGGAACGACAAGGGGCACCGGAGCCTGCCTCCCGGCTACAGG
  AGACCCCAGACCCCTGGCAGAGCCTGGACCCTTTTGACTCCTTGGAATCTAAGGTCTTCCAGAAAGGGAA
  ACCCTATTCTGTGCCACCCGGTGTGGAGGAGGCTCCAGGACAGAAGCGCAAGAGGAAGGGTGCCACCAAG
  TTGCAGGACTTCCACAAGTGGTACCTGGATGCCTATGCTGAACACCCTGACGGCAGGAGGGCTCGGCGGA
  AGGGCCCAACCTTTGCAGACATGGAAGTCCTGTACTGGAAACATGTGAAAGAACAGCTCGAGACCCTTCA
  GAAGCTGCGGAGACGTAAGATCAACGAGAGATGGCTACCTGGGGCCAAGCAGGATCTGTGGCCTACAGAG
  GAGGATCGCTTGGAAGAGTCCCTCGAGGACCTCGGGGTAGCAGCAGATGACTTTCTAGAGCCCGAGGAGT
  ACGTGGAGGAGCCTGCGGGGGTGATGCCCGAGGAAGCTGCTGACCTCGATGCAGAGGCCATGCCAGAGTC
  CCTGAGATACGAGGAGCTGGTCCGGAGAAATGTGGAACTCTTCATTGCCACCTCCCAGAAGTTTATCCAG
  GAGACAGAGCTGAGCCAACGCATCAGGGACTGGGAAGATACCATCCAGCCCCTGCTCCAGGAGCAGGAGC
  AGCATGTGCCCTTTGATATCCATATCTACGGGGACCAGTTGGCTTCACGGTTCCCCCAGCTCAATGAATG
  GTGTCCCTTTTCAGAGCTTGTAGCAGGGCAGCCTGCTTTTGAGGTGTGCCGCTCCATGCTGGCCTCCTTG
  CAACTGGCTAATGACTACACAGTGGAGATCACTCAGCAGCCAGGACTGGAGGCAGCTGTGGACACAATGT
  CTCTGAGACTGCTCACACACCAGCGAGCCCACACCCGCTTCCAGACCTATGCTGCACCATCCATGGCCCA
  GCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271601
• Insert Size: 1827 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271601.1, NP_001258530.1
• RefSeq Size: 3343 bp
• RefSeq ORF: 1827 bp
• Locus ID: 52683
• UniProt ID: Q8BSP2
• Cytogenetics: 15 44.84 cM
• Gene Summary: This gene encodes a component of the condensin-2 complex. The encoded protein may regulate the structure of mitotic chromosomes. Loss of function of this gene disrupts T-cell development. There are two pseudogenes for this gene on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (c) is longer than isoform a.