Cacnb2 (NM_001252533) Mouse Untagged Clone

CAT#: MC228449

Cacnb2 (untagged) - Mouse calcium channel, voltage-dependent, beta 2 subunit (Cacnb2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacnb2
• Synonyms: AW060387; CAB2; Cavbeta2; Cchb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228449 representing NM_001252533
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGACAGGCAGTTGGTGTCTTCTCAGACTCAATCCAGTATTCCTGGGGGTTCGGCAGACTCCTACA
  CCAGCCGCCCATCCGATTCAGATGTGTCTTTGGAAGAGGACCGGGAGGCAGTACGTAGAGAAGCTGAGCG
  GCAGGCACAGGCACAGTTGGAAAAAGCAAAGACAAAACCTGTTGCATTTGCAGTTCGGACCAATGTCAGA
  TACAGCGCAGCCCAGGAGGATGACGTTCCGGTGCCTGGCATGGCCATCTCCTTCGAGGCCAAAGATTTTC
  TGCATGTTAAAGAAAAATTTAATAATGACTGGTGGATAGGACGGCTGGTTAAAGAAGGCTGTGAAATCGG
  ATTTATTCCAAGCCCAGTCAAACTAGAGAACATGAGGCTACAGCACGAACAGAGAGCCAAGCAAGGGAAA
  TTCTATTCCAGTAAATCAGGAGGGAATTCATCATCCAGTTTGGGTGACATAGTGCCCAGTTCCAGAAAAT
  CGACACCGCCGTCATCTGCTATAGACATAGATGCTACTGGCTTAGATGCAGAAGAGAATGATATTCCAGC
  AAACCACCGCTCCCCTAAGCCCAGTGCAAACAGTGTAACATCACCCCACTCCAAAGAGAAAAGAATGCCC
  TTCTTTAAGAAGACAGAGCACACTCCTCCTTATGATGTGGTACCTTCCATGCGACCAGTGGTGCTGGTGG
  GCCCGTCTCTGAAGGGGTATGAGGTCACAGATATGATGCAAAAAGCACTGTTTGATTTTCTAAAACACAG
  ATTTGAAGGGCGGATATCCATCACAAGAGTCACTGCTGACATCTCCCTTGCCAAACGCTCAGTATTAAAC
  AATCCCAGTAAGCATGCAATCATAGAAAGATCCAACACAAGGTCCAGCTTAGCGGAAGTTCAGAGTGAAA
  TTGAAAGGATTTTTGAACTTGCAAGAACACTGCAATTGGTAGTCCTTGACGCGGATACAATTAATCACCC
  AGCTCAACTCAGTAAGACCTCTTTGGCCCCTATCATAGTGTACGTAAAGATTTCTTCTCCCAAGGTTTTA
  CAAAGGTTGATAAAATCTCGAGGGAAATCTCAAGCAAAACACCTCAATGTCCAGATGGTAGCAGCTGATA
  AACTGGCCCAATGTCCTCCGCAGGAATCATTTGATGTGATCTTGGATGAGAACCAGCTGGAGGACGCTTG
  TGAGCATCTTGCCGACTATCTGGAGGCATACTGGAAGGCCACCCACCCTCCCAGCGGTAACCTCCCCAAC
  CCTCTGCTTAGCCGGACTTTAGCCTCCTCAACTTTACCTCTGAGCCCCACCCTCGCCTCTAATTCACAGG
  GTTCTCAAGGTGATCAAAGGCCTGATCGCTCTGCTCCCCGGTCTGCTTCCCAAGCTGAAGAAGAACCTTG
  CCTAGAACCCGTCAAAAAATCCCAACACCGTTCCTCCTCAGCCACACACCAAAACCACCGCAGCGGGACA
  GGTCGAGGCCTCTCTAGGCAAGAGACGTTTGACTCTGAAACCCAAGAGAGCCGAGACTCTGCCTACGTGG
  AGCCAAAGGAAGATTATTCACATGAACATGTGGACCGGTATGTCCCACACCGCGAGCATAACCACAGAGA
  GGAGACCCACAGCAGCAATGGCCACAGACACAGGGAGTCTCGCCACCGCTCTAGGGACATGGGTCGAGAC
  CAGGACCACAATGAGTGCATCAAACAACGAAGCCGGCATAAATCTAAGGATCGCTACTGTGACAAGGAGG
  GGGAAGTAATATCCAAGAGAAGGAATGAGGCTGGCGAGTGGAACAGGGATGTATACATCCGCCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252533
• Insert Size: 1818 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252533.1, NP_001239462.1
• RefSeq Size: 3814 bp
• RefSeq ORF: 1818 bp
• Locus ID: 12296
• Cytogenetics: 2 10.7 cM
• Gene Summary: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.