Trip10 (NM_001242389) Mouse Untagged Clone

CAT#: MC228437

Trip10 (untagged) - Mouse thyroid hormone receptor interactor 10 (Trip10), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trip10
• Synonyms: AI646975; Cip4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228437 representing NM_001242389
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGGGGTACCGAGTTGTGGGATCAGTTTGAGGTGCTGGAACGCCACACGCAGTGGGGGCTGGATT
  TGTTGGACAAATACGTGAAGTTCGTGAAAGAACGCGCGGAGGTGGAGCAGGCTTATGCTAAGCAACTCCG
  GAGCCTGGTGAAAAAGTATCTTCCCAAGAGACCTACCAAAGATGACCCTGAAGTCAAGTTCAGCCAGCAG
  CAGTCATTTGTCCAGCTTCTCCAGGAGGTCAATGACTTCGCAGGCCAGAGAGAGCTGGTGGCTGAGAGCC
  TCGGTATCCGAGTGTGTCTGGAGCTGGCTAAGTATTCACAGGAGATGAAGCAGGAGAGGAAGATGCACTT
  CCAGGAAGGTCGTCGGGCCCAGCAGCAGCTGGAGAATGGCTTCAAACAGCTGGAGAATAGTAAGCGGAAG
  TTTGAACGAGATTGCCGGGAGGCTGAGAAAGCGGCTCACACTGCTGAGCGCCTAGATCAGGACATTAATG
  CCACCAAGGCGGATGTGGAGAAGGCCAAGCAGCAAGCTCACCTTCGGAACCACATGGCAGAAGAGAGCAA
  GAATGAATATGCGGCCCAGCTGCAGCGCTTCAACCGAGACCAGGCTCACTTCTACTTCTCACAGATGCCC
  CAGATATTCGATAAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCT
  TATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGAAGGTGGCCGCCGA
  GTCTGTGGATGCTAAGAACGACTCACAGGTCCTCATCGAATTACACAAGTCCGGGTTTGCCCGCCCAGGG
  GACTTGGAATTTGAAGACTTTAGCCAAGTTATCAACCGAGTGCCTTCGGACAGCAGCCTGGGCACCCCGG
  ATGGCAGGCCTGAGCTCCGAGCAGCCTCCAGCCGTAGCCGCGCCAAGCGTTGGCCTTTCGGGAAAAAGAA
  CAAGCCACGTCCCCCATCCCTGTCCCTCCTGGGGGGTCACCTACCCTCCACACTGTCTGATGGACCCTCA
  TCCCCCCGTTCTGGCCGCGACCCCTTGGCCATACTGAGCGAGATCAGTAAGTCGGTCAAACCGCGGCTAG
  CATCCTTCCGCAGCTTCCGAGGTGGCCGTGGGACCGTGGCTACCGAAGATTTCAGTCACTTGCCCCCGGA
  GCAGCAGAGAAAGCGACTTCAGCAACAGCTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAG
  AGGGAGGCCCTGAAGAAGATGAAAGATGTATATGAGAAAACACCACAAATGGGGGACCCTGCCAGCTTAG
  AGCCCCGCATTGCAGAGACCCTGGGCAACATTGAGAGGCTGAAGTTGGAAGTGCAGAAGTATGAGGCTTG
  GTTGGCAGAAGCTGAAAGCCGGGTCCTCAGTAACCGAGGGGACAGCCTAAGCCGTCACGCTAGGCCCCCT
  GATCCCCCAACTACTGCCCCACCTGATAGCAGCAGTAGCAGCACCAACAGTGGATCCCAGGACAATAAGG
  AGAGCAGCTCAGAAGAGCCCCCTTCAGAAGGCCAGGACACCCCCATCTATACTGAGTTCGATGAGGACTT
  TGAGGAGCCTGCATCCCCTATCGGCCAGTGTGTGGCTATCTACCATTTTGAAGGATCCAGCGAGGGAACC
  GTCTCCATGTCCGAGGGGGAAGACCTCAGCCTGATGGAGGAAGACAAGGGTGATGGATGGACGCGGGTCA
  GGAGGAAACAGGGAGCTGAGGGCTACGTGCCCACCTCTTACCTCCGAGTCACACTCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242389
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242389.1, NP_001229318.1
• RefSeq Size: 2458 bp
• RefSeq ORF: 1812 bp
• Locus ID: 106628
• UniProt ID: Q8CJ53
• Cytogenetics: 17 D
• Gene Summary: Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL (By similarity). Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.