Repin1 (NM_001079901) Mouse Untagged Clone

CAT#: MC228415

Repin1 (untagged) - Mouse replication initiator 1 (Repin1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Repin1
• Synonyms: AI425994; Ap4; E430037F08Rik; Zfp-464; Zfp464
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228415 representing NM_001079901
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATAGGGATGTCTTTATTGCTACAGTTTTCTCTGTCATCCGGGGGCTACCGAAGTGTGGGCCGAA
  GCAGCCGAAGTCTCCCCAGGAACATCCCCAGAAGGAGCTGGAAAAAGCCTCACCCCCAGCTCTTCAGTCT
  TCAGGCAGAGGAAGAACCGATGCTGGAACAGCGCTGCAGGGGCCCCACGGCCATGGGCCCAGCTCAGCCC
  TGGCTCTTTTCTGGGCCCTCCCAGGAGTCCTCCCAGCCCGACAGAGGGTTGAGGTACCAGGGCAAATCAG
  CTCAGCCAAGAGGCCAAACCCCAGGCAAGGTCCATCGTTGTGCCCACTGTCGGAAGCGCTTCCCGGGCTG
  GGTGGCCCTGTGGCTTCATGCTCGGCGGTGCCAGGCCCGGCTGCCTCTGCCCTGCCATGAATGCAACCAG
  CGCTTTCGCCACGCCCCCTTCTTAGCGCTGCATCTTCAGGTTCATGCTTCTGCAGTCCCCGACCTGGGTT
  TCATCTGCCACCTATGTGGGCATAGCTTCCGAGGATGGGTAGCCCTGGTTCTGCATCTGCGGGCTCACTC
  AGCTTCAAAGCGGCCCATCACTTGCCCTGAATGCGACAGACGCTTCTGGCGACAAAAACAGCTTCGAGCT
  CATCTGCGAAGGTGCCAGCCCCCTGTCCCTGAGGCCCGGCCCTTCATATGCGGCAACTGTGGCCGGAGCT
  TTGCCCAATGGGACCAACTGGTTGTTCACAAGCGGGTGCACGTGGCTGAGGCCTTGGAGGAGGCAGCAGC
  CAAAGCCCTGGGTCCTCGCCCACGAGGACGTCCCGCAGCTCCCAGGCCTGGTGGAGACGCTGTGGACCGC
  CCCTTCCAGTGTGCCTGCTGCGGCAAGCGTTTCCGCCACAAGCCCAATCTGATCGCCCACCGCCGCGTGC
  ACACTGGTGAGCGACCACACCAGTGCCCAGAGTGCGGGAAGCGTTTCACCAACAAGCCCTACCTGACCTC
  GCACCGGCGCATACATACCGGCGAGAAGCCCTACCCATGCACCGAGTGTGGCCGCCGCTTCCGCCACAAA
  CCCAACCTGTTGTCGCACAGCAAAATCCACAAGCGCTTGGAGGTCTCAGCGCAGGCTGCCCCACACCCCG
  AGAGTCACCAGATTGCAGCAGAGCCTATGGCACAACCTGCACTTGGGGTGCCCCTGGGGTCCCCGCGGAC
  CCCAGCTGAGGCACCTGCGCTCCTGCATAGCTGCTCCGACTGCGGCCGCAGCTTCCGGCTGGAGCGCTTC
  CTGCGGCTACACCAGAGGCAGCACACAGGGGAGAGGCCCTTCGCCTGCACAGAGTGTGGCAAGAACTTCG
  GCAAGAAGACGCACCTGGTGGCGCACTCACGCGTGCACTCCGGCGAACGTCCCTTCGCCTGCGAGGAGTG
  TGGTCGCCGTTTCTCACAGGGCAGCCACCTGGCAGCCCACCGGCGAGACCATGCACCAGAGAGGCCCTTC
  GTGTGCCCGGACTGCGGCAAGGCTTTCCGCCACAAGCCCTACCTGGCTGCGCACCGACGCATCCACACAG
  GCGAGAAACCCTATGTGTGTCCCGACTGTGGCAAAGCTTTCAGTCAGAAGTCCAACCTGGTGTCCCACCG
  GCGCATCCACACAGGCGAGCGGCCCTACGCCTGCCCCGACTGTGATCGTAGCTTCAGTCAGAAGTCCAAT
  CTTATCACACACCGGAAGAGCCACATCCGGGATGGCGCCTTCTGTTGTGCCATCTGTGGCCAGACCTTTG
  ATGACGAGGACCGACTCTTGATGCACCAGAAGAAGCATGATGCCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001079901
• Insert Size: 1797 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079901.1, NP_001073370.2
• RefSeq Size: 3177 bp
• RefSeq ORF: 1797 bp
• Locus ID: 58887
• UniProt ID: Q5U4E2
• Cytogenetics: 6 B2.3
• Gene Summary: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a.