Bbs12 (NM_001255992) Mouse Untagged Clone

CAT#: MC228404

Bbs12 (untagged) - Mouse Bardet-Biedl syndrome 12 (human) (Bbs12), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbs12
• Synonyms: Gm407; Gm721; Gm1805
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228404 representing NM_001255992
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAAGGCTTGAACTCTTGCATTGAAGCAGTAGTTTCTCTTCAAGTACCCATACACAATGTATTTG
  ACCACATGGACAACACAAGTACAGTTTACAAACTTGAAACAGTTAATGCCACTTTGTGTCCTTTTCTACA
  AGACCCTTCAGGTTCTGGGTTGTTACAGGAAAAGCGTGATTTCAAAGATGCTACATCTCCGTTATTGTCC
  ACTTACAGTCTTTCTGGGAGACATGCTGAGTCACCCAAATTCTTCAAACCTCAGAATAATCTTGAAACAG
  AAAAAAACACATTGCAAGTTTTGAAAAACAATCTATATACAGATTCCTTCTGCAAAAAGTCAGCACTAGC
  TCACAGTAGGCATTTTAATAGGACAGATAATAGCCACTGGATAAGCCGACATGATGGATTTCTAGAACAA
  CTTGAATCAACTCCGAAAGTACTTAGATGTAATGATTTTGGGGAGTTAGCAGTTGGCTTGAGCCATGGAG
  ATCACAGCAGCATGGCATTGGCCAAAGCAGCAGTGAGGTTGCAGTGGCAGAGTCTGTGTCTGCAGCAAGC
  CAACTGGATGGCACCCTTTATGTTTGATATTTCAAGACTTCTCACTTGCTGTATTCCAGGTCTACCTGAA
  ACTTTTTCCCGTGTTGGTCTAGGATATGTCACTTTTGTTACCATGTCTAGTATTACTCTTATCAAGGAAT
  TGCAGGATCAGCCTTTCCGAGTGATTCTCATTGAGGGTGACCTCACAGAGAGTTACCGACACCTGGGATT
  TAATAAGTCTGTAAATATTAAGACTAAGTTAGATAGTGGGGAGCTTTCAGAAGATAGTGCAGAAGAACTG
  TGGACAAATCATGTGTTACAGGTGTTAATCCAGTTCAATGTGACCCTCATCTTGGTACAAGGAAGTGTAT
  CAGAACACTTGACTGAAAAATGCATGCACAGTAAGCGGCTGGTAATTGGGGCAGTGAATGGCAGTGTGTT
  GCAGGCATTTGCAGAGGCCACAAGAGCAGTGCCAGTAGCCTATGTTACACAAGTGAATGAAGACTGTGTA
  GGCAGCGGGGTCTCTGTGACCTTCTGGATGAGTCCTCATGATATAAACAGGAGCAACAGAATAGCAATCT
  TGTTAACAGCAGAAGGAATTAATTTGATTACAGCAGTACTTACTAGTCCAGCAAGTGCTCAGATGGAAAC
  CAAGGAAGACAGGTTCTGGTCTTGTGTGTATCGTTTATATCATGCCCTAAAAGAAGAGAAGGTCTTCCTT
  GGCGGTGGTGCTGTTGAATTTTTATGTCTTAGCCATCTTCAAATTCTTGCTGAGCAATCTTTAAATAGAG
  GAAACCATGCTTGTTTAGGATGGCTTCCTGATTCTTCCTCTTGGATGGCCTCATCTCTGTCCGTCTACAG
  ACCTACTGTGCTGAAGTCCCTGGCAGGTGGGTGGCATGAATTCCTGTCAGCTATCATGTGTAACACTGCC
  ACTCACCCATCAGCAGTGGAAGCTAGGACATTCATTCAACAACATGTACAAAATGCCATTGACTCTGGCT
  CCCCTTCATCTTACATCTTGAGTGAATATAGTAAACTAAGTAGTGGAGTTTTTCATTCAGGTATTTCAGA
  TAACCTGGAGCTGGTTCCAAGAGTTTATGATACTGTTACACCAAAGATTGAAGCATGGCGCCGAGCATTG
  GATGTAGTGCTCTTAGTGCTTCAGACAGACAGTGAAATAATTACTGGACTTGTACACACAGAGATGAATT
  CACAGGAATTAGATGGAGTTTTATTTTTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001255992
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255992.1, NP_001242921.1
• RefSeq Size: 2347 bp
• RefSeq ORF: 1782 bp
• Locus ID: 241950
• Cytogenetics: 3 B
• Gene Summary: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate splice site, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.